Broad‐spectrum virus reduction in red cell concentrates using INACTINE™ PEN110 chemistry

Lazo, Aris; Tassello, Jodie; Vasudevacharya, Jayarama; Öhagen, Åsa; Gibaja, Veronica; Kramer, Ed; Marmorato, A.; Billia-Shaveet, D.; Purmal, Andrei A.; Brown, F.; Chapman, John

doi:10.1046/j.1423-0410.2002.00234.x

Cited by 37 publications

(46 citation statements)

References 34 publications

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“…There is extensive literature describing the inactivation of caliciviruses by other classes of disinfectants, including aldehydes [8,31,72,79,88,112,113,114], quaternary ammonium compounds [8,69,75,76,79,81,82,85,103,107,113], surfactants [8,69,73,112,113], sodium hydroxide [31,79], and other chemicals [103,115,116]. The calicivirus inactivation efficacy information for these agents determined in coupon studies (virus dried onto surfaces) and in solution studies is summarized in Table 8.…”

Section: Resultsmentioning

confidence: 99%

“…A few other chemical treatments that have displayed efficacy for inactivating caliciviruses include β-propiolactone (0.1%, 60 min at 22 °C [115]), and the proprietary alkylating agent INACTINE ® PEN 110 (0.1%, 3 hr at 22 °C [116]). These have displayed inactivation efficacy in very specific applications (viral inactivation in IgG [but not cryo-poor plasma], and red blood cell suspensions, respectively), and their utility for inactivation in other applications (matrices) has not been established.…”

Section: Resultsmentioning

confidence: 99%

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Inactivation of Caliciviruses

Nims¹,

Plavsic²

2013

Pharmaceuticals

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The Caliciviridae family of viruses contains clinically important human and animal pathogens, as well as vesivirus 2117, a known contaminant of biopharmaceutical manufacturing processes employing Chinese hamster cells. An extensive literature exists for inactivation of various animal caliciviruses, especially feline calicivirus and murine norovirus. The caliciviruses are susceptible to wet heat inactivation at temperatures in excess of 60 °C with contact times of 30 min or greater, to UV-C inactivation at fluence ≥30 mJ/cm2, to high pressure processing >200 MPa for >5 min at 4 °C, and to certain photodynamic inactivation approaches. The enteric caliciviruses (e.g.; noroviruses) display resistance to inactivation by low pH, while the non-enteric species (e.g.; feline calicivirus) are much more susceptible. The caliciviruses are inactivated by a variety of chemicals, including alcohols, oxidizing agents, aldehydes, and β-propiolactone. As with inactivation of viruses in general, inactivation of caliciviruses by the various approaches may be matrix-, temperature-, and/or contact time-dependent. The susceptibilities of the caliciviruses to the various physical and chemical inactivation approaches are generally similar to those displayed by other small, non-enveloped viruses, with the exception that the parvoviruses and circoviruses may require higher temperatures for inactivation, while these families appear to be more susceptible to UV-C inactivation than are the caliciviruses.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Inactivation of Caliciviruses

Nims¹,

Plavsic²

2013

Pharmaceuticals

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“…It was the only one of these inactivators that did not require light for it to be activated (12) . It did not damage platelets or erythrocytes, but had the disadvantages that saline washes to remove its residues were required after the treatment and that its use led to the formation of antibodies against red cells (Table 1) (2,8,9) .…”

Section: Resultsmentioning

confidence: 99%

“…This results in the spontaneous opening of the imidazole ring of the guanine and produces a modified base (O 6 -methylguanine) that acts as a potent inactivator of DNA polymerase (2) . Modification of the base may also produce non-basic sites, which represent a stop sign for DNA and RNA polymerase (8) .…”

Section: Introductionmentioning

confidence: 99%

Viral inactivation in hemotherapy: systematic review on inactivators with action on nucleic acids

Sobral¹,

Barros²,

Gomes³

et al. 2012

Revista Brasileira De Hematologia E Hemoterapia

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The aim of this study was to conduct a systematic review on the photoinactivators used in hemotherapy, with action on viral genomes. The SciELO, Science Direct, PubMed and Lilacs databases were searched for articles. The inclusion criterion was that these should be articles on inactivators with action on genetic material that had been published between 2000 and 2010. The key words used in identifying such articles were "hemovigilance", "viral inactivation", "photodynamics", "chemoprevention" and "transfusion safety". Twenty-four articles on viral photoinactivation were found with the main photoinactivators covered being: methylene blue, amotosalen HCl, S-303 frangible anchor linker effector (FRALE), riboflavin and inactin. The results showed that methylene blue has currently been studied least, because it diminishes coagulation factors and fibrinogen. Riboflavin has been studied most because it is a photoinactivator of endogenous origin and has few collateral effects. Amotosalen HCl is effective for platelets and is also used on plasma, but may cause changes both to plasma and to platelets, although these are not significant for hemostasis. S-303 FRALE may lead to neoantigens in erythrocytes and is less indicated for red-cell treatment; in such cases, PEN 110 is recommended. Thus, none of the methods for pathogen reduction is effective for all classes of agents and for all blood components, but despite the high cost, these photoinactivators may diminish the risk of blood-transmitted diseases.

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“…This chemical targets on the lipid envelope of virus causing final destruction of both envelope and virus capsid (Heerklotz and Seelig 2007). Inactine is an ethyleneiminederived substance that binds and modifies nucleic acids being effective against both enveloped and non-enveloped viruses (Lazo et al 2002). Â-propiolactone targets on several viral structural components (e.g., nucleic acids, proteins; Lawrence 2000).…”

Section: Methods For Viral Clearancementioning

confidence: 99%

Application of methods for viral clearance in stem cell production

Cobo

2007

In Vitro Cell.Dev.Biol.-Animal

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Regenerative medicine therapies will allow in the future the transplant of cells of human origin in some diseases that until now have been incurable. The assurance of the safety and quality, especially from a microbiological point of view, is very important for these therapeutic products. Depending on the starting material, there are several sources of pathogen presence, mainly human viruses. Also, the use of feeders of animal origin as layers in which the stem cells can grow may permit the transmission of animal pathogens to these cells. However, cell sources are limited due to the low availability of spare in vitro fecundation human embryos and the low rate of success in the derivation of human stem cell lines. Thus, in several cases, it will be necessary to evaluate the possibility of removing or inactivating these microorganisms. In this paper, we summarize the main methods of viral clearance and we have provided an overview of the main features taking into account in the viral clearance techniques.

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Broad‐spectrum virus reduction in red cell concentrates using INACTINE™ PEN110 chemistry

Cited by 37 publications

References 34 publications

Inactivation of Caliciviruses

Inactivation of Caliciviruses

Viral inactivation in hemotherapy: systematic review on inactivators with action on nucleic acids

Application of methods for viral clearance in stem cell production

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