Uncrewed surface vehicles (USVs) equipped with echosounders have the potential to replace or enhance acoustic observations from conventional research vessels (RVs), increase spatial and temporal coverage, and reduce cost and carbon emission. We discuss the objectives, system requirements, infrastructure, and regulations for using USVs with echosounders to conduct ecological experiments, acoustic-trawl surveys, and long-term monitoring. We present four example applications of USVs with lengths <8 m, and highlight some advantages and disadvantages relative to RV-based data acquisitions. Sail-driven USVs operate continuously for months and are more mature than motorized USVs, but they are slower. To maintain the pace of an RV, multiple sail-powered USVs sample in coordination. In comparison, motorized USVs can travel as fast as RVs and therefore may facilitate a combined survey, interleaving USV and RV transects, with RV-based biological sampling. Important considerations for all USVs include platform design, noise and transducer motion mitigation, communications and operations infrastructure, onboard data processing, biological sampling approach, and legal requirements. This technology is evolving and applied in multiple disciplines, but further development and institutional commitment are needed to allow USVs equipped with echosounders to become ubiquitous and useful components of a worldwide network of autonomous ocean observation platforms.