Broadband microchamber for electrical detection of live and dead biological cells

Single Cell Microdosimetric Studies Comparing Ideal and Measured Nanosecond Pulsed Electric Fields

“…NsPEFs of 20 ns, monopolar and bipolar ( measured across the electrodes (nearly 15 μ matched microchamber [9], these pulses are id (S_nsPEF) on the basis of a recent literature c The nanosecond measurement setup was b 8110A to generate the pulse. A digital sampl Agilent 86100A 50-GHz, was used in configuration like in [9] to monitoring th reflected and the transmitted signal (real broadband divider with 50-ȍ input/output imp Additionally, the scattering (S) para microchamber loaded with the biological ce were measured in order to define a transfer current flowing across the electrodes and h cell.…”

“…A digital sampl Agilent 86100A 50-GHz, was used in configuration like in [9] to monitoring th reflected and the transmitted signal (real broadband divider with 50-ȍ input/output imp Additionally, the scattering (S) para microchamber loaded with the biological ce were measured in order to define a transfer current flowing across the electrodes and h cell. On the basis of the scattering parameter d CAL e cell membrane ested in [6].The ell compartments T on =20 ns), were μm wide) of the dentified as short classification [5].…”

“…nucleus) effects due to ideal and measured nsPEFs. The real waveforms are delivered by a broadband matched device as the one proposed in [9].…”

Broadband Electrical Detection of Individual Biological Cells

“…Despite these challenges, broadband electrical detection at or near the single-cell level has recently been demonstrated [12], [13]. Cells need to be kept alive in solutions that tend to absorb microwave signals.…”

“…Therefore, microfluidics must be used to minimize the solution volume while delivering cells smoothly. Cell trapping either mechanically [12] or electrically [13] between the detection electrodes maximizes the cell-to-sample volume ratio while allowing relatively wide microfluidic channels to be used to prevent clogging. Whereas electrical trapping does not perturb fluid flow as much as mechanical trapping does, in both cases, the trapped cell can be repeatedly sampled to increase the signal-to-noise ratio.…”