Broadening the genetic base of Abyssinian mustard (Brassica carinata A. Braun) through introgression of genes from related allotetraploid species

Sheikh, Faheem A.; Banga, Sashi; Banga, Surindar Singh

doi:10.5424/sjar/2014123-5365

Cited by 6 publications

(3 citation statements)

References 21 publications

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“…Our identification of Bc subgenome dominance in the B. carinata genome will significantly aid efforts in its improvement as a biofuel resource. Efforts for crop improvement, such as addressing the plant's tall stature and long life cycle, have been thwarted by the species' lack of genetic diversity (Sheikh et al, 2014). Our comprehensive characterization of the B. carinata could advance crop improvement efforts, including those of other Brassica spp.…”

Section: Unprecedented Retention Of Transposon Insertions In Gene Regionsmentioning

confidence: 99%

The last missing piece of the Triangle of U: the evolution of the tetraploid Brassica carinata genome

Yim

Swain

et al. 2022

Preprint

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Ethiopian mustard (Brassica carinata) is an ancient crop with significant potential for expanded cultivation as a biodiesel feedstock. The remarkable stress resilience of B. carinata and desirable seed fatty acid profile addresses the ongoing food vs. fuel debate as the crop is productive on marginal lands otherwise not suitable for even closely related species. B. carinata is one of six key Brassica spp. that share three major genomes: three diploid species (AA, BB, CC) that spontaneously hybridized in a pairwise manner, forming three allotetraploid species (AABB, AACC, and BBCC). Each of these genomes has been researched extensively, except for that of B. carinata. In the present study, we report a high-quality, 1.31 Gbp genome with 156.9-fold sequencing coverage for B. carinata var. Gomenzer, completing and confirming the classic Triangle of U, a theory of the evolutionary relationships among these six species that arose almost a century ago. Our assembly provides insights into the genomic features that give rise to B. carinata's superior agronomic traits for developing more climate-resilient Brassica crops with excellent oil production. Notably, we identified an expansion of transcription factor networks and agronomically-important gene families. Completing the Triangle of U comparative genomics platform allowed us to examine the dynamics of polyploid evolution and the role of subgenome dominance in domestication and agronomical improvement.

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Section: Unprecedented Retention Of Transposon Insertions In Gene Regionsmentioning

confidence: 99%

The last missing piece of the Triangle of U: the evolution of the tetraploid Brassica carinata genome

Yim

Swain

et al. 2022

Preprint

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“…Moreover, B. carinata (BBCC) is endowed with many agronomically desirable traits such as resistance/tolerance to abiotic stresses (drought and heat); and biotic stresses, viz., aphid, Sclerotinia rot, white rust, Alternaria blight, blackleg, and powdery mildew [15]. Successful attempts of interspecific hybridization were made to transfer favourable alleles from B. carinata to B. rapa [16,17], B. napus [18], and B. juncea [7,14,[19][20][21]. Nikzad et al [22] used B. oleracea to exploit heterosis in hybrids between B. napus and B. oleracea; and suggested that the C genome of B. oleracea has potential to improve the seed yield in hybrid cultivars.…”

Section: Introductionmentioning

confidence: 99%

Introgression of Heterotic Genomic Segments from Brassica carinata into Brassica juncea for Enhancing Productivity

Vasisth

Singh

Limbalkar

et al. 2023

Plants

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Interspecific hybridization resulted in the creation of B. juncea introgression lines (ILs) generated from B. carinata with increased productivity and adaptability. Forty ILs were crossed with their respective B. juncea recipient parents to generate introgression line hybrids (ILHs) and the common tester (SEJ 8) was used to generate test hybrids (THs). Mid-parent heterosis in ILHs and standard heterosis in THs were calculated for eight yield and yield-related traits. Heterotic genomic regions were dissected using ten ILs with significant mid-parent heterosis in ILHs and standard heterosis in THs for seed yield. A high level of heterosis for seed yield was contributed by 1000 seed weight (13.48%) in D31_ILHs and by total siliquae/plant (14.01%) and siliqua length (10.56%) in PM30_ILHs. The heterotic ILs of DRMRIJ 31 and Pusa Mustard 30 were examined using polymorphic SNPs between the parents, and a total of 254 and 335 introgressed heterotic segments were identified, respectively. This investigation discovered potential genes, viz., PUB10, glutathione S transferase, TT4, SGT, FLA3, AP2/ERF, SANT4, MYB, and UDP-glucosyl transferase 73B3 that were previously reported to regulate yield-related traits. The heterozygosity of the FLA3 gene significantly improved siliqua length and seeds per siliqua in ILHs of Pusa Mustard 30. This research proved that interspecific hybridization is an effective means of increasing the diversity of cultivated species by introducing new genetic variants and improving the level of heterosis.

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“…Although this species has tall plant stature, poor seed characteristics, and takes longer to mature (Supplementary Table 1), when compared to B. juncea, it possesses many agronomically desirable traits including resistance or tolerance to most of the abiotic and biotic stresses like drought, heat, aphid, Sclerotinia rot, white rust, Alternaria black spot, blackleg, and powdery mildew (Raman et al, 2017;Thakur et al, 2019), which are otherwise eliminated from the predominantly cultivated Brassica species during domestication. Therefore, efforts toward interspecific hybridisation were made in the past to transfer desirable traits from B. carinata to cultivated Brassica species viz., B. rapa (Jiang et al, 2007;Choudhary et al, 2008), B. napus (Navabi et al, 2011;Sheikh et al, 2014), and B. juncea (Getinet et al, 1994;Sheikh et al, 2014;Singh K. H. et al, 2015). B. carinata is known to be the most drought tolerant among cultivated Brassica species as it develops sufficient biomass and seed yield even under the water deficit stress conditions.…”

Section: Introductionmentioning

confidence: 99%

Deployment of Brassica carinata A. Braun Derived Brassica juncea (L.) Czern. Lines for Improving Heterosis and Water Use Efficiency Under Water Deficit Stress Conditions

et al. 2021

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Among Brassica species, Ethiopian mustard (Brassica carinata A. Braun) is known to tolerate most abiotic stresses, including drought. Drought caused by low and erratic rainfall in semi-arid regions consistently challenges rapeseed mustard productivity. Development of B. carinata-derived lines (CDLs) in Brassica juncea (L.) Czern. nuclear background, carrying genomic segments from B. carinata, are expected to tolerate moisture deficit stress conditions. The present study was, thus, aimed to establish the phenomenon “heterosis” for drought tolerance and water use efficiency by evaluating 105 hybrids developed from intermating 15 CDLs in half diallel fashion. Data on 17 seed yield and yield contributing traits were recorded under two different environments, viz., irrigated and rainfed conditions. Traits under study were found to be governed by both additive and non-additive types of gene action. Average degree of dominance was higher (>2) for yield and yield contributing traits, viz., secondary branches/plant, point to first siliqua on main shoot, total siliquae/plant, 1,000-seed weight, seed yield/plant, biological yield, harvest index, and seed yield/hectare under rainfed conditions, clearly indicating that higher productivity under drought conditions can be realised through the development of hybrids. Out of 15, highly significant general combining ability (GCA) effects for seven CDLs were observed under rainfed condition. Furthermore, nine and six hybrids expressed highly significant specific combining ability (SCA) effects and > 50% heterobeltiosis for yield contributing traits under rainfed and irrigated conditions, respectively. Water use efficiency (WUE) of parental CDLs and hybrids varied from 2.05 to 2.57 kg m–3 under rainfed, while 1.10 to 1.28 kg m–3 under irrigated conditions. Hybrids expressed higher WUE than parental lines under both water regimes. Furthermore, selection indices such as drought tolerance index (DTI) and mean relative performance (MRP) were identified to be efficient in the selection of productive CDLs and hybrids under drought conditions. Nine hybrids, identified as highly productive in the present study, can further be exploited for improving the yield of Indian mustard in drought-prone areas. Usefulness of interspecific hybridisation in the development of B. carinata-derived B. juncea lines for improving heterosis and WUE is, thus, well demonstrated through the present study.

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Broadening the genetic base of Abyssinian mustard (Brassica carinata A. Braun) through introgression of genes from related allotetraploid species

Cited by 6 publications

References 21 publications

The last missing piece of the Triangle of U: the evolution of the tetraploid Brassica carinata genome

The last missing piece of the Triangle of U: the evolution of the tetraploid Brassica carinata genome

Introgression of Heterotic Genomic Segments from Brassica carinata into Brassica juncea for Enhancing Productivity

Deployment of Brassica carinata A. Braun Derived Brassica juncea (L.) Czern. Lines for Improving Heterosis and Water Use Efficiency Under Water Deficit Stress Conditions

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