Broadening the tools for studying sand fly breeding habitats: A novel molecular approach for the detection of phlebotomine larval DNA in soil substrates

Giantsis, Ioannis A.; Chaskopoulou, Alexandra

doi:10.1016/j.actatropica.2018.11.008

Cited by 8 publications

(6 citation statements)

References 22 publications

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“…A bulk of the literature identified in this analysis focuses on mosquitoes—the discussions on common vector breeding grounds [ 52 , 106 , 107 , 108 ] and the efficacy of insect-killing traps seldom involve other disease vectors [ 128 ]. There is a need for research into effective methods to better understand the breeding habitat ecology of sand flies in immature stages, which will facilitate the development of targeted control strategies such as source reduction, which are not yet possible as sand fly larvae can be difficult to detect, in contrast to other vectors such as mosquitoes [ 173 , 174 , 175 ]. Similarly, in the case of insect-killing traps, only limited studies demonstrate their potential in targeting sand flies in addition to mosquitoes [ 129 ], and evidence on tick elimination by the traps is lacking entirely.…”

Section: Discussionmentioning

confidence: 99%

Narrative Review on Health-EDRM Primary Prevention Measures for Vector-Borne Diseases

Chan

Sham

Shahzada

et al. 2020

IJERPH

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Climate change is expanding the global at-risk population for vector-borne diseases (VBDs). The World Health Organization (WHO) health emergency and disaster risk management (health-EDRM) framework emphasises the importance of primary prevention of biological hazards and its value in protecting against VBDs. The framework encourages stakeholder coordination and information sharing, though there is still a need to reinforce prevention and recovery within disaster management. This keyword-search based narrative literature review searched databases PubMed, Google Scholar, Embase and Medline between January 2000 and May 2020, and identified 134 publications. In total, 10 health-EDRM primary prevention measures are summarised at three levels (personal, environmental and household). Enabling factor, limiting factors, co-benefits and strength of evidence were identified. Current studies on primary prevention measures for VBDs focus on health risk-reduction, with minimal evaluation of actual disease reduction. Although prevention against mosquito-borne diseases, notably malaria, has been well-studied, research on other vectors and VBDs remains limited. Other gaps included the limited evidence pertaining to prevention in resource-poor settings and the efficacy of alternatives, discrepancies amongst agencies’ recommendations, and limited studies on the impact of technological advancements and habitat change on VBD prevalence. Health-EDRM primary prevention measures for VBDs require high-priority research to facilitate multifaceted, multi-sectoral, coordinated responses that will enable effective risk mitigation.

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Section: Discussionmentioning

confidence: 99%

Narrative Review on Health-EDRM Primary Prevention Measures for Vector-Borne Diseases

Chan

Sham

Shahzada

et al. 2020

IJERPH

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“…Sand flies breed in a wide variety of terrestrial sites that are not easily detectable nor well characterized, making the use of larvicides difficult ( Table 1 ). However, an increasing effort to develop tools for easy identification of sand fly larval habitats [ 53 ] may greatly enhance the precision and efficacy of larviciding interventions.…”

Section: Methodsmentioning

confidence: 99%

Chemical control and insecticide resistance status of sand fly vectors worldwide

et al. 2021

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Background Phlebotomine sand flies are prominent vectors of Leishmania parasites that cause leishmaniasis, which comes second to malaria in terms of parasitic causative fatalities globally. In the absence of human vaccines, sand fly chemical-based vector control is a key component of leishmaniasis control efforts. Methods and findings We performed a literature review on the current interventions, primarily, insecticide-based used for sand fly control, as well as the global insecticide resistance (IR) status of the main sand fly vector species. Indoor insecticidal interventions, such as residual spraying and treated bed nets are the most widely deployed, while several alternative control strategies are also used in certain settings and/or are under evaluation. IR has been sporadically detected in sand flies in India and other regions, using non-standardized diagnostic bioassays. Molecular studies are limited to monitoring of known pyrethroid resistance mutations (kdr), which are present at high frequencies in certain regions. Conclusions As the leishmaniasis burden remains a major problem at a global scale, evidence-based rational use of insecticidal interventions is required to meet public health demands. Standardized bioassays and molecular markers are a prerequisite for this task, albeit are lagging behind. Experiences from other disease vectors underscore the need for the implementation of appropriate IR management (IRM) programs, in the framework of integrated vector management (IVM). The implementation of alternative strategies seems context- and case-specific, with key eco-epidemiological parameters yet to be investigated. New biotechnology-based control approaches might also come into play in the near future to further reinforce sand fly/leishmaniasis control efforts.

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“…Multiplex qPCR was verified to provide 100% efficiency and an R 2 value of 0.99 for parasite detection, such as Giardia and Cryptosporidium [62], and between 92.5% to 105.8% with an R 2 value of >0.98 in detecting Listeria monocytogenes serotype in ready-to-eat meat products [37]. Multiplex qPCR has been mainly used to identify different species within sample, with a benefit of enabling detection as low as one organism/40 mL of soil in the case of sand flies [63]. On the other hand, use of multiplex ddPCR benefits assays of atypical transcripts such as detecting the presence of the fusion transcript BCR-ABL1, a marker for chronic myeloid leukemia [64].…”

Section: Multiplex Ddpcrmentioning

confidence: 99%

“…It would be best used in situations where there are no well-developed positive standards available, or the target gene is present in low concentrations, or when detection attempts using qPCR are not successful. Since ddPCR does not require standards for each run, it is suitable for analysis of many samples of low abundance with fewer preparation steps, which facilitate efficiency and productivity [63].…”

Section: Applicabilitymentioning

confidence: 99%

Competitiveness of Quantitative Polymerase Chain Reaction (qPCR) and Droplet Digital Polymerase Chain Reaction (ddPCR) Technologies, with a Particular Focus on Detection of Antibiotic Resistance Genes (ARGs)

Park

Rana

Sung

et al. 2021

Applied Microbiology

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With fast-growing polymerase chain reaction (PCR) technologies and various application methods, the technique has benefited science and medical fields. While having strengths and limitations on each technology, there are not many studies comparing the efficiency and specificity of PCR technologies. The objective of this review is to summarize a large amount of scattered information on PCR technologies focused on the two majorly used technologies: qPCR (quantitative polymerase chain reaction) and ddPCR (droplet-digital polymerase chain reaction). Here we analyze and compare the two methods for (1) efficiency, (2) range of detection and limitations under different disciplines and gene targets, (3) optimization, and (4) status on antibiotic resistance genes (ARGs) analysis. It has been identified that the range of detection and quantification limit varies depending on the PCR method and the type of sample. Careful optimization of target gene analysis is essential for building robust analysis for both qPCR and ddPCR. In our era where mutation of genes may lead to a pandemic of viral infectious disease or antibiotic resistance-induced health threats, this study hopes to set guidelines for meticulous detection, quantification, and analysis to help future prevention and protection of global health, the economy, and ecosystems.

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Broadening the tools for studying sand fly breeding habitats: A novel molecular approach for the detection of phlebotomine larval DNA in soil substrates

Cited by 8 publications

References 22 publications

Narrative Review on Health-EDRM Primary Prevention Measures for Vector-Borne Diseases

Narrative Review on Health-EDRM Primary Prevention Measures for Vector-Borne Diseases

Chemical control and insecticide resistance status of sand fly vectors worldwide

Competitiveness of Quantitative Polymerase Chain Reaction (qPCR) and Droplet Digital Polymerase Chain Reaction (ddPCR) Technologies, with a Particular Focus on Detection of Antibiotic Resistance Genes (ARGs)

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