Bromelain-loaded chitosan nanofibers prepared by electrospinning method for burn wound healing in animal models

Bayat, Samaneh; Amiri, Nafise; Pishavar, Elham; Kalalinia, Fatemeh; Movaffagh, Jebrail; Hashemi, Maryam

doi:10.1016/j.lfs.2019.05.028

Cited by 126 publications

(72 citation statements)

References 41 publications

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“…As a comparison, the Neurotensin‐loaded PLGA/CNC composite nanofibers (Zheng et al, 2018) had significant tissue regeneration after the 14th day and the re‐epithelialization continued to up to three layers, whereas in the present study, the re‐epithelialization after treatment by nanofibers containing 8 wt % DDAC, continued to exist five epithelium layers with 1 week more healing process. As a comparison, according to a recent study on wound treatment using CS nanofibers and Bromelain (Bayat et al, 2019), in spite of proper wound healing, the lower epithelialization and higher inflammation in the histology of the intended treatment show feeble healing whereas present results express more operative treatment. Overall review of study results confirmed that best healing signs were seen in treatment with nanofibers containing DDAC 8 wt %.…”

Section: Discussionmentioning

confidence: 56%

Physical and biological properties of blend‐electrospun polycaprolactone/chitosan‐based wound dressings loaded with N‐decyl‐N, N‐dimethyl‐1‐decanaminium chloride: An in vitro and in vivo study

et al. 2020

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Dual-pump electrospinning of antibacterial N-decyl-N, N-dimethyl-1-decanaminiumchloride (DDAC)-loaded polycaprolactone (PCL) nanofibers, and chitosan (CS)/polyethylene-oxide (PEO)-based wound dressings with hydrophilic and hydrophobic properties to eliminate and absorb pathogenic bacteria from wound surface besides antibacterial action and to support wound healing and accelerate its process. Physicochemical properties of the prepared nanofibrous mat as well as antibacterial, cytotoxicity, and cell compatibility were studied. The full-thickness excisional wound healing properties up to 3 weeks using hematoxylin and eosin and Masson-trichrome staining were investigated. Addition of DDAC to CS/PEO-PCL mats decreased the diameter of the nanofibers, which is a crucial property for wound healing as large surface area per volume ratio of nanofibers, in addition to proper cell adhesion, increases loading of DDAC in mats and leads to increased cell viability and eliminating Gram-positive bacteria at in vitro studies. In vivo studies showed DDAC-loaded CS/PEO-PCL mats increased epithelialization and angiogenesis and decreased the inflammation according to histological results. We demonstrated that hydrophobic PCL/DDAC mats, besides antibacterial properties of DDAC, absorbed and eliminated the hydrophobic pathological microorganisms, whereas the hydrophilic nanofibers consisted of CS/PEO, increased the cell adhesion and proliferation due to positive charge of CS. Finally, we were able to increase the wound healing quality by using multifunctional wound dressing. CS/PEO-PCL containing 8 wt % of DDAC nanofibrous mats is promising as a wound dressing for wound management due to the favorable interactions between the pathogenic bacteria and PCL/CS-based wound dressing.

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Section: Discussionmentioning

confidence: 56%

Physical and biological properties of blend‐electrospun polycaprolactone/chitosan‐based wound dressings loaded with N‐decyl‐N, N‐dimethyl‐1‐decanaminium chloride: An in vitro and in vivo study

et al. 2020

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“…The synthetic polymers used in wound dressing formulations include poly(ethylene glycol) (PEG)/poly(ethylene oxide)(PEO), poly(vinyl pyrrolidone) (PVP), poly(vinyl alcohol) (PVA), [ 15 ], poly(hydroxyethyl methacrylate) (PHEMA), polyurethanes (PUs), poly (α-esters) [e.g., poly(lactic-co-glycolic acid) (PLGA), polyglycolic acid (PGA), polylactide (PLA), poly(ε-caprolactone)(PCL)] etc. [ 16 , 17 , 18 ]. These polymers exhibit good mechanical properties but suffer from poor biocompatibility.…”

Section: Introductionmentioning

confidence: 99%

Polymer-Based Materials Loaded with Curcumin for Wound Healing Applications

2020

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Some of the currently used wound dressings have interesting features such as excellent porosity, good water-absorbing capacity, moderate water vapor transmission rate, high drug loading efficiency, and good capability to provide a moist environment, but they are limited in terms of antimicrobial properties. Their inability to protect the wound from microbial invasion results in wound exposure to microbial infections, resulting in a delayed wound healing process. Furthermore, some wound dressings are loaded with synthetic antibiotics that can cause adverse side effects on the patients. Natural-based compounds exhibit unique features such as good biocompatibility, reduced toxicity, etc. Curcumin, one such natural-based compound, has demonstrated several biological activities such as anticancer, antibacterial and antioxidant properties. Its good antibacterial and antioxidant activity make it beneficial for the treatment of wounds. Several researchers have developed different types of polymer-based wound dressings which were loaded with curcumin. These wound dressings displayed excellent features such as good biocompatibility, induction of skin regeneration, accelerated wound healing processes and excellent antioxidant and antibacterial activity. This review will be focused on the in vitro and in vivo therapeutic outcomes of wound dressings loaded with curcumin.

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“…For the treatment of severe burns, 2% ( w/v ) bromelain (a debriding agent commonly used in burn treatment) was added to electrospun chitosan nanofibers [ 118 ]. When applied to burns, this material reduced the burn wound area and significantly increased the density of collagen tissue compared to ordinary passive burn dressings.…”

Section: Chitosan-based Nanofibers In Biomedical Applicationsmentioning

confidence: 99%

Recent Developments in Chitosan-Based Micro/Nanofibers for Sustainable Food Packaging, Smart Textiles, Cosmeceuticals, and Biomedical Applications

et al. 2021

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Chitosan has many useful intrinsic properties (e.g., non-toxicity, antibacterial properties, and biodegradability) and can be processed into high-surface-area nanofiber constructs for a broad range of sustainable research and commercial applications. These nanofibers can be further functionalized with bioactive agents. In the food industry, for example, edible films can be formed from chitosan-based composite fibers filled with nanoparticles, exhibiting excellent antioxidant and antimicrobial properties for a variety of products. Processing ‘pure’ chitosan into nanofibers can be challenging due to its cationic nature and high crystallinity; therefore, chitosan is often modified or blended with other materials to improve its processability and tailor its performance to specific needs. Chitosan can be blended with a variety of natural and synthetic polymers and processed into fibers while maintaining many of its intrinsic properties that are important for textile, cosmeceutical, and biomedical applications. The abundance of amine groups in the chemical structure of chitosan allows for facile modification (e.g., into soluble derivatives) and the binding of negatively charged domains. In particular, high-surface-area chitosan nanofibers are effective in binding negatively charged biomolecules. Recent developments of chitosan-based nanofibers with biological activities for various applications in biomedical, food packaging, and textiles are discussed herein.

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Bromelain-loaded chitosan nanofibers prepared by electrospinning method for burn wound healing in animal models

Cited by 126 publications

References 41 publications

Physical and biological properties of blend‐electrospun polycaprolactone/chitosan‐based wound dressings loaded with N‐decyl‐N, N‐dimethyl‐1‐decanaminium chloride: An in vitro and in vivo study

Physical and biological properties of blend‐electrospun polycaprolactone/chitosan‐based wound dressings loaded with N‐decyl‐N, N‐dimethyl‐1‐decanaminium chloride: An in vitro and in vivo study

Polymer-Based Materials Loaded with Curcumin for Wound Healing Applications

Recent Developments in Chitosan-Based Micro/Nanofibers for Sustainable Food Packaging, Smart Textiles, Cosmeceuticals, and Biomedical Applications

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