Bromelain nanoemulsion formulation resulting from partial purification of pineapple core (Ananas comosus [L.] Merr) and in vitro testing as antiinflammation

Pertiwi, Indah; Setiasih, Siswati; Handayani, Sri; Hudiyono, Sumi

doi:10.1063/5.0001343

Cited by 3 publications

(5 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…174 The formulation parameters of bromelain nanoformulations need to be optimized to achieve maximum safety, efficacy, and commercial viability. 175…”

Section: Food and Function Reviewmentioning

confidence: 99%

Bromelain: a review of its mechanisms, pharmacological effects and potential applications

Kumar¹,

Mangla²,

Javed³

et al. 2023

Food Funct.

View full text Add to dashboard Cite

show abstract

“…174 The formulation parameters of bromelain nanoformulations need to be optimized to achieve maximum safety, efficacy, and commercial viability. 175…”

Section: Food and Function Reviewmentioning

confidence: 99%

Bromelain: a review of its mechanisms, pharmacological effects and potential applications

Kumar¹,

Mangla²,

Javed³

et al. 2023

Food Funct.

View full text Add to dashboard Cite

show abstract

“…Based on the cycling test method, the nanoemulsion formed is stable without phase separation and crystallization of active substances. The alteration of pH after six cycles did not change significantly, from 5.44±0.00 to 5.42±0.00, the centrifugal force was given around 3850 rpm for five hours, which is equivalent to the gravity force for a year [1]. Based on the results obtained, the nanoemulsion base showed no phase separation, which means the stability was equivalent for a year.…”

Section: Stability Test Of Nanoemulsionmentioning

confidence: 79%

“…The O/W type is marked with the methylene blue evenly distributed in nanoemulsion. Meanwhile, if there are only spots, that is confirmed that the nanoemulsion type is water in oil (W/O) [1]. Particle size determination is one of the factors that will affect the absorption of a drug through the skin.…”

Section: Characterization Of Nanoemulsionmentioning

confidence: 93%

“…The oil in water (o/w) nanoemulsion will dissolve and diffuse the methylene blue into the whole water region evenly. Meanwhile, in the water and oil (w/o) nanoemulsion, methylene blue will cluster on its surface [1].…”

Section: Characterization Of Nanoemulsionmentioning

confidence: 99%

“…This problem triggers the researcher to find a natural active compound as an alternative to anti-inflammatory therapy. Indonesia is the fifth largest producer of pineapple in the world [1], which the amount of pineapple production will also increase the number of its wastes. One of that wastes is the pineapple core, which contains a protein-active substance called bromelain.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Evaluation of Activity and Stability of Isolated Bromelain From Pineapple Cores (Ananas Comosus [L.] Merr) and in Vitro Penetration Test of Nanoemulsion Topical Base

Rohmah

Prabowo

Setiasih³

et al. 2021

Int J App Pharm

View full text Add to dashboard Cite

Objective: Oral administration of bromelain as an anti-inflammation therapy still faces several challenges, such as the risk of contact with the gastric fluid and its low absorption rate. Therefore, bromelain isolated from the pineapple core will be formulated as a topical base in a nanoemulsion to increase its stability, activity, and ability to penetrate the skin. Methods: Bromelain was isolated from pineapple core using ammonium sulfate precipitation and dialysis, and then the isolated fraction was loaded into topical nanoemulsion for subsequent evaluation of its characteristics, stability, enzymatic activity, and for in vitro study of penetration using Franz diffusion cell. Results: The highest specific activity of bromelain fractions found in ammonium sulfate concentration is around 20–50%. After being dialyzed, the bromelain fraction showed an increase in specific activity 2.78-fold as compared to crude extract. The characteristics of bromelain nanoemulsion showed a globule size of 21.37±1.8 nm with a polydispersity index (pdI) 0.323±0.049, oil in water (o/w) type, and the type of rheology was plastic flow. The nanoemulsion stability base was observed, and there was no phase separation after centrifugation. Bromelain in nanoemulsion base showed a proteolytic activity of 5.00 U/ml with a protein content of 154.28 mg/l. In vitro penetration studies using Franz diffusion cell for 8 h showed isolated bromelain in nanoemulsion base has a total cumulative number value of 1386.94 μg/cm2 with penetrated velocity/flux (J) of 45.93 μg/cm2 h. Conclusion: The results showed promise for bromelain loaded into nanoemulsion as a vehicle for topically administered therapeutic enzymes.

show abstract

Development of a Novel Pomegranate Polysaccharide Nanoemulsion Formulation with Anti-Inflammatory, Antioxidant, and Antitumor Properties

Hoseny

Soliman

Fahmy

et al. 2023

CDD

View full text Add to dashboard Cite

Background: Colorectal cancer is one of the most serious gastrointestinal cancers in Africa and its prevention is a pronounced challenge in contemporary medicine worldwide. Objective: The present study aimed to develop a nanoemulsion drug delivery system using pomegranate polysaccharides (PGPs) as alternative cancer remedy, then evaluated its biological activities. Objective: The present study aimed to develop a nanoemulsion drug delivery system using pomegranate polysaccharides (PGPs) as alternative cancer remedy, then evaluated its biological activities. Methods: The PGPs yield and chemical composition were evaluated, then a PGPs nanoemulsion (PGPs-NE) was prepared using the self-emulsification technique with an oil phase. The physicochemical characterization of PGPs-NE was then analyzed. The in vitro antioxidant, anti-inflammatory activities, and antitumor potency of PGPs and PGPs-NE were also evaluated. Results: The PGPs yield was 10%. The total sugar and protein content of PGPs was 44.66 mg/dl and 19.83µg/ml, respectively. PGPs was mainly composed of five monosaccharides including fructose, glucose, galactose, rhamnose, and arabinose. Concerning physiochemical characterization, the formulated PGPs-NE had three optical absorption bands at 202, 204 and 207nm and a transmittance of 80%. Its average hydrodynamic particle size was 9.5nm, with a PDI of less than 0.2 and a negative zeta potential (30.6 mV). The spherical shape of PGPs-NE was confirmed by a transmission electron microscope study, with an average size of less than 50 nm. Additionally, the method used to prepare the PGPs-NE formulation provided a high entrapment efficiency (92.82%). The current study disclosed that PGPs-NE exhibited strong antioxidant, anti-inflammatory, and antitumor agent potency compared to that of free PGPs. Conclusion: These promising current findings provide evidence for the possible efficacy of novel PGPs-NE as an alternative treatment for CRC.

show abstract

Bromelain nanoemulsion formulation resulting from partial purification of pineapple core (Ananas comosus [L.] Merr) and in vitro testing as antiinflammation

Cited by 3 publications

References 15 publications

Bromelain: a review of its mechanisms, pharmacological effects and potential applications

Bromelain: a review of its mechanisms, pharmacological effects and potential applications

The Evaluation of Activity and Stability of Isolated Bromelain From Pineapple Cores (Ananas Comosus [L.] Merr) and in Vitro Penetration Test of Nanoemulsion Topical Base

Development of a Novel Pomegranate Polysaccharide Nanoemulsion Formulation with Anti-Inflammatory, Antioxidant, and Antitumor Properties

Contact Info

Product

Resources

About