• High concentrations of PCDD/Fs and PBDEs were found at wastewater discharge points.• PCDD/F and PBDE concentrations were low to moderate compared to data in literature.• The concentration difference between fish species was attributed to feeding habits.• The fish were fit for human consumption with regard to PCDD/Fs and PBDEs. a b s t r a c t a r t i c l e i n f o Polychlorinated dibenzo-p-dioxins/furans (PCDD/Fs) and polybrominated diphenyl ethers (PBDEs) were analyzed in sediments and fish from the Murchison Bay of Lake Victoria by high resolution gas chromatography/ high resolution mass spectrometry (HRGC/HRMS). Average concentrations of total (Σ) PCDD/Fs and ΣPBDEs in sediments ranged from 68.8 to 479 pg g −1 dry weight (dw) and 60.8 to 179 pg g −1 dw, respectively. Contamination levels of sedimentary PCDD/Fs and PBDEs were low to moderate compared to other urbanized regions worldwide. The concentrations in different fish species (Nile perch; Lates niloticus and Nile tilapia; Oreochromis niloticus) were 5.32 to 49.0 pg g −1 wet weight (ww) for PCDD/Fs and 59.3 to 495 pg g −1 ww for PBDEs. Higher concentrations of the pollutants were found in L. niloticus than O. niloticus, which could be attributed to species differences in feeding habits and lifestyles. World Health Organization-toxic equivalents (WHO 2005 -TEQs) for PCDD/Fs ranged from 0.08 to 0.33 pg TEQ g −1 dw and 0.001-0.14 pg TEQ g −1 ww in sediments and fish, respectively. The TEQ values were low compared to the data for fresh water fish reported in literature and within a permissible level of 3.5 pg g −1 ww recommended by the European Commission. Based on the Commission set value and minimum risk level criteria formulated by the Agency for Toxic Substances and Disease Registry, the fish from the Murchison Bay was fit for human consumption.