Bromophenol (5‐bromo‐3,4‐dihydroxybenzaldehyde) isolated from red alga Polysiphonia morrowii inhibits adipogenesis by regulating expression of adipogenic transcription factors and AMP‐activated protein kinase activation in 3T3‐L1 adipocytes

Abstract

The aim of the present study was to investigate the effect of 5‐bromo‐3,4‐dihydroxybenzaldehyde (BD) isolated from Polysiphonia morrowii on adipogenesis and differentiation of 3T3‐L1 preadipocytes into mature adipocytes and its possible mechanism of action. Levels of lipid accumulation and triglyceride were significantly lower in BD treated cells than those in untreated cells. In addition, BD treatment reduced protein expression levels of peroxisome proliferator‐activated receptor‐γ, CCAAT/enhancer‐binding proteins α, and sterol regulatory element‐binding protein 1 compared with control (no treatment). It also reduced expression levels of adiponectin, leptin, fatty acid synthase, and fatty acid binding protein 4. AMP‐activated protein kinase activation was found to be one specific mechanism involved in the effect of BD. These results demonstrate that BD possesses inhibitory effect on adipogenesis through activating AMP‐activated protein kinase signal pathway.

Effects of UV Radiation on Photosynthesis, Antioxidant Capacity and the Accumulation of Bioactive Compounds in Gracilariopsis longissima, Hydropuntia cornea and Halopithys incurva (Rhodophyta)

“…), Polysiphonia morrowii (Ko et al. ) and Symphyocladia latiuscula (Wang et al. ), among many others, besides having different bioactive capacities, they have antioxidant capacity (Xu et al.…”

Seaweed Phenolics: From Extraction to Applications

“…Bromophenols (BP) ( Figure 2) are secondary metabolites with ecological functions, such as chemical defense and deterrence, with studies revealing a wide variety of beneficial ecological activities [33,61]. BP are common to all major algal groups [61]; they were first isolated from the red algae Neorhodomela larix (formerly known as Rhodomela larix) [64] and thereafter identified and isolated from all taxonomic groups of marine macroalgae, such as red [64][65][66], green [67][68][69], and brown algae [70][71][72][73].…”

“…Ko et al [66] isolated the 5-bromo-3,4-dihydroxybenzaldehyde from Polysiphonia morrowii and studied the effect and mechanism of action of this compound in adipogenesis and the differentiation of 3T3-L1 preadipocytes, demonstrating that this bromophenol targets the peroxisome proliferator-activated receptor-γ expression levels and also the CCAAT/enhancer-binding proteins α and sterol regulatory element-binding protein 1. However, the main mechanism is under the AMP-activated protein kinase signal pathway, inhibiting the adipogenesis, in vitro assay.…”

Progress of Bromophenols in Marine Algae from 2011 to 2020: Structure, Bioactivities, and Applications

“…Besides the hypoglycemic activity, some marine BPs have the potential to treat obesity. BP 2.30 (also 3.8 , 4.9 , 5.1 , and Scheme 2 ), isolated from the red alga Polysiphonia morrowii , could inhibit adipogenesis by regulating expression of adipogenic transcription factors and AMP-activated protein kinase activation in 3T3-L1 adipocytes and subsequently inhibited intracellular lipid accumulation and triglyceride level, suggesting the potential to treat obesity [ 82 ].…”