Bronchial Biopsy in Chronic Bronchitis and Asthma

Glynn, A. A.; Michaels, LeAnn

doi:10.1136/thx.15.2.142

Cited by 172 publications

(60 citation statements)

References 11 publications

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“…The use of a single endobronchial biopsy A number of biopsy studies of airway inflammation appear to have relied upon the use of a single biopsy specimen [8,[13][14][15]. In the present study, mean intracase correlations between mast cell density in biopsy sections and adjacent airway transverse sections were very low.…”

Section: Inflammation In Bronchial Biopsies ML Carroll Et Almentioning

confidence: 60%

“…Endobronchial biopsy has been widely used as a research tool to assess airway inflammation in asthma [3,4,[6][7][8][9][10][11][12][13][14][15][16]22]. Its use as a tool in clinical research has recently become the subject of review [20].…”

Section: Discussionmentioning

confidence: 99%

“…Since most biopsy studies reporting the density of inflammatory cells have been in mild cases of asthma [3,4,[6][7][8][9][10][11][12][13][14][15][16]22], comparisons with post mortem studies are effectively limited to the small number of such studies that have compared transverse sections from cases of less severe, nonfatal asthma and controls [23,24]. Results of biopsy studies in mild asthma have not been entirely consistent with either results of the post mortem studies using cases of nonfatal asthma, or with each other.…”

mentioning

confidence: 97%

“…Biopsy studies of airway inflammation have generally used only two or three biopsies per case to enumerate a particular cell type [3,4,[6][7][8][9][10][12][13][14][15][16], and have often relied on 2D cell profile methods, expressing density as cells per area [4,[8][9][10][11][12][14][15][16].…”

Section: Inflammation In Bronchial Biopsies ML Carroll Et Almentioning

confidence: 99%

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Do bronchial biopsies represent mast cell density in airways? A stereological study

Carroll

Carroll²

2006

Eur Respir J

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Endobronchial biopsy specimens may not adequately represent inflammatory cell counts throughout the airway wall. The present study aimed to compare mast cell density in biopsies and airway sections using both stereological and nonstereological methods.Post mortem biopsies and adjacent transverse sections were obtained from a mean of five proximal airways per case in 10 subjects who had died of nonrespiratory causes. Tryptasepositive mast cells were measured stereologically in 30-mm sections and nonstereologically in 5-mm sections using an optical disector (cells?mm ), respectively. Reference areas included the inner and total airway wall and to 100 mm below the basement membrane.Case means, based on four or more biopsy sites, significantly correlated with those on transverse sections for counts over the inner airway wall only, using both stereological and nonstereological methods. Cells?mm -3 and cells?mm -2 were significantly correlated within all reference areas. When endobronchial biopsies are obtained from at least four proximal airways per case, intersubject comparisons of mean mast cell density in the inner airway wall are as well represented by counts on biopsies as they are on transverse sections. This is the case using either threedimensional, stereological or two-dimensional, nonstereological methods.

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Section: Inflammation In Bronchial Biopsies ML Carroll Et Almentioning

confidence: 60%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 97%

Section: Inflammation In Bronchial Biopsies ML Carroll Et Almentioning

confidence: 99%

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Do bronchial biopsies represent mast cell density in airways? A stereological study

Carroll

Carroll²

2006

Eur Respir J

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“…Epithelial cell derangements are associated with a number of human inflammatory diseases (13). Desquamated epithelial cells can be seen in sputum in asthmatics, often in clusters forming LTcola bodies (2), and individuals dying of asthma often demonstrate detachment of epithelial cells from the underlying BM as seen in biopsies and post-mortem studies (I), in addition, light and electron microscopic studies of human asthmatic airways during life often demonstrate epithelial cell damage and/or J: Fig.…”

Section: Discussionmentioning

confidence: 99%

Study of human epithelial cell detachment and damage: Development of a model

et al. 1989

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Summary Detachment of epithelial structures from underlying basement membrane (BM) represents an important component of a number of human disease processes e.g. airway and alveolar diseases, gastrointestinal ulceration, and retinal diseases. This study describes a method of evaluating human epithelial cell detachment from BM that is simple, rapid, inexpensive, quantifiable and which, because it utilizes BM rather than tissue culture plastic, more closely mimics the in vivo situation than other methods. In this model human amnionic epithelial cells attached to their underlying BM are isolated from fresh placentae and mounted in a multi-well chemotaxis assembly. These membranes can be studied with the epithelial cell monolayer intact. Protease-induced detachment of the epithelial cells from the underlying BM was readily quantifiable using light microscopy and spectroscopy. Following removal of the native amnionic epithelial cells, immunoperoxidase staining for the BM attachment proteins laminin, fibronectin, and type IV collagen demonstrated that these molecules remain intact. The BM could also be used as an attachment surface to reconstitute other epithelial cell monolaycrs. Cultured human amnionic cells and human respiratory epithelial cells were both able to attach to the denuded BM in the absence of serum (% attachment = 85 ± 15% and 92 ± 8% respectively, P=0 8). Natural BM was a better substrate for epithelial cell attachment than tissue culture plastic in that, in the absence of serum, cultured epithelial ceil attachment to tissue culture plastic was 20 ± 4% of the value for BM {/'<0 05). Furthermore, cells attached to plastic adhered less effectively than to BM in that trypsin concentrations required to induce 50% cell detachment were 0 72 ± 0 4 for plastic and 62 ± 13 BAEE U/mL for BM iP<000\).In view of the complex protein interactions known to be involved in the anchorage of human epithelial cells to BM, it is likely this model will be a useful tool for evaluating the mechanisms underlying human epithelial cell attachment and detachment in a variety of normal and disease situations.

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Asthmatic Deaths

Connell¹

1971

JAMA

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Bronchial Biopsy in Chronic Bronchitis and Asthma

Cited by 172 publications

References 11 publications

Do bronchial biopsies represent mast cell density in airways? A stereological study

Do bronchial biopsies represent mast cell density in airways? A stereological study

Study of human epithelial cell detachment and damage: Development of a model

Asthmatic Deaths

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