Bronchial microbiome of severe COPD patients colonised by Pseudomonas aeruginosa

Millares, Laura; Ferrari, Rafaela Gomes; Gallego, Miguel; García-Núñez, Marian; Pérez‐Brocal, Vicente; Espasa, Mateu; Pomares, Xavier; Montón, Concepción; Moya, Andrés; Monsó, Eduard

doi:10.1007/s10096-013-2044-0

Cited by 123 publications

(109 citation statements)

References 41 publications

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“…In agreement with previous studies, COPD exacerbation events appear to be associated with decreased microbial diversity and increased proportion of Proteobacteria [8,23]. In addition, there was a remarkable proliferation of Moraxella in a subgroup of subjects during exacerbations (36 out of 87 subjects).…”

Section: Network Analysis Reveals Potential Microbiota Interactionssupporting

confidence: 92%

Lung microbiome dynamics in COPD exacerbations

et al. 2016

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Increasing evidence suggests that the lung microbiome plays an important role in chronic obstructive pulmonary disease (COPD) severity. However, the dynamics of the lung microbiome during COPD exacerbations and its potential role in disease aetiology remain poorly understood.We completed a longitudinal 16S ribosomal RNA survey of the lung microbiome on 476 sputum samples collected from 87 subjects with COPD at four visits defined as stable state, exacerbation, 2 weeks post-therapy and 6 weeks recovery.Our analysis revealed a dynamic lung microbiota where changes appeared to be associated with exacerbation events and indicative of specific exacerbation phenotypes. Antibiotic and steroid treatments appear to have differential effects on the lung microbiome. We depict a microbial interaction network for the lung microbiome and suggest that perturbation of a few bacterial operational taxonomic units, in particular Haemophilus spp., could greatly impact the overall microbial community structure. Furthermore, several serum and sputum biomarkers, in particular sputum interleukin-8, appear to be highly correlated with the structure and diversity of the microbiome.Our study furthers the understanding of lung microbiome dynamics in COPD patients and highlights its potential as a biomarker, and possibly a target, for future respiratory therapeutics. @ERSpublications Lung microbiome changes are associated with COPD exacerbation events and implicated in host inflammatory responses

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Section: Network Analysis Reveals Potential Microbiota Interactionssupporting

confidence: 92%

Lung microbiome dynamics in COPD exacerbations

et al. 2016

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“…Interestingly, in Pseudomonas-colonized patients, the microbiome changed to a microbiome similar to non-Pseudomonas colonized patients during exacerbations [45]. Analysis of the microbiome provided some clues about the relationship between viruses and bacteria in the pathogenesis of exacerbations of COPD.…”

Section: Microbioma and Exacerbations Of Chronic Obstructive Pulmonarmentioning

confidence: 99%

Role of infection in exacerbations of chronic obstructive pulmonary disease

Miravitlles

Anzueto²

2015

Current Opinion in Pulmonary Medicine

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COPD patients have alterations in their lung microbiome that may result in chronic infection with potentially pathogenic microorganisms (PPMs) even in periods of clinical stability and associated with a higher frequency of bacterial exacerbations. Patients with this infective phenotype may require a personalized approach to therapy with the use of short-term or long-term antibiotic treatment in addition to the usual treatment for COPD.

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“…Thus, the examination of the respiratory microbiome confirms that a causal bacterial pathogen may keep unnoticed by conventional microbiology in some exacerbations, while the culture identifies microorganisms that are only colonizers. This equivocal information of conventional microbiology has been reported in up to twenty percent of patients chronically colonized by Pseudomonas aeruginosa (4,27).…”

Section: Exacerbationmentioning

confidence: 99%

“…Microbiome analyses show high relative abundances of specific genera, which may be considered etiologic, for most of the exacerbations, while the remaining flora do not change significantly (26)(27)(28)(29). Furthermore, exacerbations are not only related to overrepresentations of isolated genera, but also associated with collateral changes of microbiome composition as a whole, not always identifiable through the measurement of relative abundances (30,31).…”

Section: Exacerbationmentioning

confidence: 99%

Microbiome in chronic obstructive pulmonary disease

Monsó¹

2017

Ann. Transl. Med.

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The introduction of culture-independent techniques for the microbiological analysis of respiratory samples has confirmed that the respiratory system hosts a large number of microorganisms, which include a wide range of bacteria. The regular exposure to tobacco smoke changes the microbiome in healthy smokers, first in the oropharynx, increasing the presence of a restricted number of genera which attain high relative abundance, a pattern that may be considered as dysbiosis. In chronic obstructive pulmonary disease (COPD), microbiome analyses of sputum samples have demonstrated an important decline in bacterial diversity, with a change to a restricted flora with an overrepresentation of the Proteobacteria phylum, which include most of the bacteria commonly considered as potentially pathogenic microorganisms, paralleled by a decline in the relative abundance of microorganisms part of the Firmicutes phylum. In exacerbations, specific bacteria overrepresented in microbiome analyses and potentially causal of the acute episode may not be recovered by sputum culture, while colonizing microorganisms grow easily, in spite that their relative abundance have not changed from previous stability. This situation has been described in patients showing chronic colonization by Pseudomonas aeruginosa, who suffer from exacerbations that in most cases are due to other PPMs, in spite of the persistence of positive cultures for the colonizing Pseudomonas strains.Interaction between different microorganisms can be addressed through microbiome analyses, and functional metagenomics, that describes the genomic potential of the community, has shown that, in spite that the bronchial microbiome as a whole may not change significantly, clear changes in carbohydrate metabolism, cancer, cell growth and death, transport and catabolism pathways often appear during exacerbations. These functional changes may be important because through them the resident community as a whole show its power to modify important metabolic patterns.

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Bronchial microbiome of severe COPD patients colonised by Pseudomonas aeruginosa

Cited by 123 publications

References 41 publications

Lung microbiome dynamics in COPD exacerbations

Lung microbiome dynamics in COPD exacerbations

Role of infection in exacerbations of chronic obstructive pulmonary disease

Microbiome in chronic obstructive pulmonary disease

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