Bronchial Provocation Studies in Farmers Allergic to Storage Mites

Ingram, Charles G.; Symington, I. S.; Jeffrey, I. G.; Cuthbert, O. D.

doi:10.1016/s0140-6736(79)92814-9

Cited by 66 publications

(20 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Storage mites as a cause of allergic disorders have been reported in several publications [3][4][5][10][11][12][13]. In contrast to the vast literature on allergens of the house dust mite Dermatophagoides pteronyssinus [14,15], few reports describe the responsible allergens of storage mites.…”

Section: Introductionmentioning

confidence: 99%

Demonstration of Allergen Components in the Storage Mite <i>Lepidoglyphus destructor</i> by an Immunoblotting Technique

Johansson

Hage‐Hamsten²,

Johansson³

1988

Int Arch Allergy Immunol

View full text Add to dashboard Cite

The allergen composition of crude extracts of the storage mite Lepidoglyphus destructor were investigated by sodium dodecylsulphate polyacrylamide gel electrophoresis and immunoblotting. The allergen components were detected by sera from allergic farmers with a positive radioallergosorbent test to L. destructor. The allergen-antibody complexes were visualized by rabbit anti-IgE and β-galactosidase-labelled sheep antirabbit IgG in a chromogenic substrate. Six out of the forty-three sera also had IgE antibodies to the culture medium, which were neutralized by addition of medium antigens before the demonstration of mite allergen components. A total of 11 allergen components were identified. Two of them were found to be major allergen components, identified by more than 50% of the 43 sera used in the study. These 2 IgE-binding components had molecular weights of approximately 18,000 and 16,000 daltons, respectively.

show abstract

Section: Introductionmentioning

confidence: 99%

Demonstration of Allergen Components in the Storage Mite <i>Lepidoglyphus destructor</i> by an Immunoblotting Technique

Johansson

Hage‐Hamsten²,

Johansson³

1988

Int Arch Allergy Immunol

View full text Add to dashboard Cite

show abstract

“…The respiratory system, which seems to be the primary target of grain dust, reacts with a variety of symptoms such as cough, wheezing, tightness of chest, and dyspnea. Wheezing and shortness of breath could be due to reversible airways obstruction since an asthmatic reaction has been both observed clinically (12,14) and provoked in challenge studies (15)(16)(17)(18)(19)(20)(21)(22). Conjunctivitis (2,3), sldn itching and rash (3,6,10) have also been reported as well as grain fever which is characterized by malaise, headache, muscle ache, tiredness, feverish feeling and chills accompanied by rise in body temperatures and white cell count (2,6,7,23,24).…”

Section: Introductionmentioning

confidence: 99%

Acute Symptoms Following Exposure to Grain Dust in Farming

Manfreda¹,

Holford-Strevens²,

Cheang³

et al. 1986

Environmental Health Perspectives

View full text Add to dashboard Cite

History of acute symptoms (cough, wheezing, shortness of breath, fever, stuffy nose, and skin itching/ rash) following exposure to grain dust was obtained from 661 male and 535 female current and former farmers. These symptoms were relatively common: 60% of male and 25% of female farmers reported at least one such symptom on exposure to grain dust. Association of cough, wheezing, shortness of breath, and stuffy nose with skin reactivity and capacity to form IgE is consistent with an allergic nature of these symptoms. Barley and oats dust were perceived as dust most often producing symptoms. On the other hand, grain fever showed a different pattern, i.e., it was not associated with either skin reactivity or total IgE. Smoking might modify the susceptibility to react to grain dust with symptoms. Only those who reported wheezing on exposure to grain dust may have an increased risk to develop chronic airflow obstruction.

show abstract

“…Some of the obstructive symptom s which occurred in to 070 of the farmers in the pre sent study may have been of a similar nature. Storage mites in hay (14), as well as pollen and animal dander, may also have contributed. The obstructive symptoms were not related to the febrile episodes, but were positively correlated with the serum IgE values.…”

mentioning

confidence: 99%