Bronchoalveolar lavage fluid cytokine, cytology and IgE allergen in horses with equine asthma

Hansen, Sanni; Otten, Nina Dam; Birch, Karin; Skovgaard, Kerstin; Hopster‐Iversen, Charlotte; Fjeldborg, Julie

doi:10.1016/j.vetimm.2019.109976

Cited by 30 publications

(32 citation statements)

References 52 publications

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“…Also consistent with previous reports, almost all differentially expressed genes were upregulated, with only three being downregulated. The differential expression of IL1RN in the group of horses with airway inflammation (Supplementary Data 8 ), was consistent with the findings of Hansen et al who reported an association between IL1RN expression in BALF derived cells and the neutrophilic form of MMEA 65 . Similarly, genes cysteine rich secretory protein LCCL domain containing 2 ( CRISPLD2) and transglutaminase 3 ( TGM3) were also present in BALF cells derived from horses with neutrophilic form of MMEA 64 .…”

Section: Discussionsupporting

confidence: 90%

Training associated alterations in equine respiratory immunity using a multiomics comparative approach

Karagianni

Kurian

Cillán‐García

et al. 2022

Sci Rep

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Neutrophilic airway inflammation is highly prevalent in racehorses in training, with the term mild to moderate equine asthma (MMEA) being applied to the majority of such cases. Our proposed study is largely derived from the strong association between MMEA in racehorses and their entry into a race training program. The objectives of this study are to characterise the effect of training on the local pulmonary immune system by defining the gene and protein expression of tracheal wash (TW) derived samples from Thoroughbred racehorses prior to and following commencement of race training. Multiomics analysis detected 2138 differentially expressed genes and 260 proteins during the training period. Gene and protein sets were enriched for biological processes related to acute phase response, oxidative stress, haemopoietic processes, as well as to immune response and inflammation. This study demonstrated TW samples to represent a rich source of airway cells, protein and RNA to study airway immunity in the horse and highlighted the benefits of a multiomics methodological approach to studying the dynamics of equine airway immunity. Findings likely reflect the known associations between race-training and both airway inflammation and bleeding, offering further insight into the potential mechanisms which underpin training associated airway inflammation.

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Section: Discussionsupporting

confidence: 90%

Training associated alterations in equine respiratory immunity using a multiomics comparative approach

Karagianni

Kurian

Cillán‐García

et al. 2022

Sci Rep

View full text Add to dashboard Cite

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“…Also consistent with previous reports, almost all differentially expressed genes were upregulated, with only three being downregulated. The differential expression of IL1RN in the group of horses with airway in ammation (Supplementary Data 8), was consistent with the ndings of Hansen et al (2020) who reported an association between IL1RN expression in BALF derived cells and the neutrophilic form of MMEA 64 . Similarly, genes cysteine rich secretory protein LCCL domain containing 2 (CRISPLD2) and transglutaminase 3 (TGM3) were also present in BALF cells derived from horses with neutrophilic form of MMEA 63 .…”

Section: Transcriptomic Pro Le Of Racehorses With High Neutrophil Count On Tw Samplessupporting

confidence: 90%

Training Associated Alterations in Equine Respiratory Immunity Using a Multiomics Comparative Approach

Karagianni

Kurian

Cillán‐García

et al. 2021

Preprint

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Neutrophilic airway inflammation is highly prevalent in racehorses in training, with the term mild to moderate equine asthma (MMEA) being applied to the majority of such cases. Our proposed study is largely derived from the strong association between MMEA in racehorses and their entry into a race training program. The objectives of this study are to characterise the effect of training on the local pulmonary immune system by defining the gene and protein expression of tracheal wash (TW) derived samples from Thoroughbred racehorses prior to and following commencement of race training. Multiomics analysis detected 2,138 differentially expressed genes and 260 proteins during the training period. Gene and protein sets were enriched for biological processes related to acute phase response, oxidative stress, haemopoietic processes, as well as to immune response and inflammation. This study demonstrated TW samples to represent a rich source of airway cells, protein and RNA to study airway immunity in the horse and highlighted the benefits of a multiomics methodological approach to studying the dynamics of equine airway immunity. Findings likely reflect the known associations between race-training and both airway inflammation and bleeding, offering further insight into the potential mechanisms which underpin training associated airway inflammation.

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“…Several studies reporting increased expression levels of IL-1β and IL-8 in humans with neutrophilic asthma agree with these results [ 39 , 40 ]. Interestingly, these pathways and signature genes also are consistent with findings in horses with sEA, including recent transcriptomic analyses of the bronchial epithelium of affected horses relative to healthy horses in response to antigen challenge [ 22 ] and several other studies reporting overexpressed IL-1β and IL-8 [ 17 , 41 , 42 , 43 , 44 ].…”

Section: Discussionsupporting

confidence: 79%

An Integrative miRNA-mRNA Expression Analysis Reveals Striking Transcriptomic Similarities between Severe Equine Asthma and Specific Asthma Endotypes in Humans

Hulliger

Pacholewska

Vargas

et al. 2020

Genes

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Severe equine asthma is an incurable obstructive respiratory condition affecting 10–15% of horses in temperate climates. Upon exposure to airborne antigens from hay feeding, affected horses show neutrophilic airway inflammation and bronchoconstriction, leading to increased respiratory effort. The resulting implications range from welfare concerns to economic impacts on equestrian sports and horse breeding. Immunological and pathophysiological characteristics of severe equine asthma show important parallels with allergic and severe neutrophilic human asthma. Our study aimed at investigating regulatory networks underlying the pathophysiology of the disease by profiling miRNA and mRNA expression in lung tissue samples from asthmatic horses compared with healthy controls. We sequenced small RNAs and mRNAs from lungs of seven asthmatic horses in exacerbation, five affected horses in remission, and eight healthy control horses. Our comprehensive differential expression analyses, combined with the miRNA–mRNA negative correlation approach, revealed a strong similarity on the transcriptomic level between severe equine asthma and severe neutrophilic asthma in humans, potentially through affecting Th17 cell differentiation. This study also showed that several dysregulated miRNAs and mRNAs are involved in airway remodeling. These results present a starting point for a better transcriptomic understanding of severe equine asthma and its similarities to asthma in humans.

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Bronchoalveolar lavage fluid cytokine, cytology and IgE allergen in horses with equine asthma

Cited by 30 publications

References 52 publications

Training associated alterations in equine respiratory immunity using a multiomics comparative approach

Training associated alterations in equine respiratory immunity using a multiomics comparative approach

Training Associated Alterations in Equine Respiratory Immunity Using a Multiomics Comparative Approach

An Integrative miRNA-mRNA Expression Analysis Reveals Striking Transcriptomic Similarities between Severe Equine Asthma and Specific Asthma Endotypes in Humans

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