Bronchoalveolar lavage protein patterns in children with malignancies, immunosuppression, fever and pulmonary infiltrates

Neumann, Manfred; Bredow, Christina von; Ratjen, Félix; Griese, Matthias

doi:10.1002/1615-9861(200206)2:6<683::aid-prot683>3.0.co;2-z

Cited by 27 publications

(13 citation statements)

References 28 publications

(40 reference statements)

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“…The current master gel of BALF proteins comprises more than 1200 spots visualized by silver staining [402]. Identification of proteins on 2-D gels was initially performed using immunoblotting, Edman sequencing, or matching to a reference gel [403,404], and this was significantly improved through the use of advanced MS identification techniques [405][406][407][408][409]. A large-scale proteomic analysis by using immunoaffinity depletion, SDS-PAGE fractionation, protein in-gel digestion, and subsequent nano-LC-MS/MS was reported for identification and semiquantitation of more than 1500 distinct proteins in BALF.…”

Section: Analysis and Characterization Of Balf Proteomementioning

confidence: 99%

“…Gel-solin secretion was found increased three-fold in the airway surface liquid of epithelia treated with IL-4, suggesting that gelsolin might improve the fluidity of airway surface liquid in asthma by breaking down filamentous actin that may be released in large amounts by dying cells during inflammation [417,404]. In addition, a number of 2-DE studies were conducted to monitor the proteomic changes in BALF of patients suffering from lupus erythematosis, Wegener's granulomatosis, lipoid pneumonia, chronic eosinophilic pneumonia, asbestosis, bacterial pneumonia, hypersensitivity pneumonitis, malignancies, immunosuppression, and smoking [399][400][401][402]407,[418][419][420][421].…”

Section: Application To Human Diseases Detectionmentioning

confidence: 99%

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Human body fluid proteome analysis

2006

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The focus of this article is to review the recent advances in proteome analysis of human body fluids, including plasma/serum, urine, cerebrospinal fluid, saliva, bronchoalveolar lavage fluid, synovial fluid, nipple aspirate fluid, tear fluid, and amniotic fluid, as well as its applications to human disease biomarker discovery. We aim to summarize the proteomics technologies currently used for global identification and quantification of body fluid proteins, and elaborate the putative biomarkers discovered for a variety of human diseases through human body fluid proteome (HBFP) analysis. Some critical concerns and perspectives in this emerging field are also discussed. With the advances made in proteomics technologies, the impact of HBFP analysis in the search for clinically relevant disease biomarkers would be realized in the future.

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Section: Analysis and Characterization Of Balf Proteomementioning

confidence: 99%

Section: Application To Human Diseases Detectionmentioning

confidence: 99%

Human body fluid proteome analysis

2006

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“…A study by Guo et al utilized one-dimensional (1D) electrophoresis with mass spectrometry (MS) and two-dimensional liquid chromatography-MS (LC-MS) to define the airway proteome of a healthy mouse (20). In addition, a proteomics approach has been used to define the proteins present in the airway in patients with a variety of conditions (3,4,7,15,16,39,41,44,46,50,59,61,62,65,70). However, little is known about the effects of acute infection on the airway proteome and the ways these effects change over time.…”

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confidence: 99%

Staphylococcus aureus Elicits Marked Alterations in the Airway Proteome during Early Pneumonia

et al. 2008

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Pneumonia caused by Staphylococcus aureus is a growing concern in the health care community. We hypothesized that characterization of the early innate immune response to bacteria in the lungs would provide insight into the mechanisms used by the host to protect itself from infection. An adult mouse model of Staphylococcus aureus pneumonia was utilized to define the early events in the innate immune response and to assess the changes in the airway proteome during the first 6 h of pneumonia. S. aureus actively replicated in the lungs of mice inoculated intranasally under anesthesia to cause significant morbidity and mortality. By 6 h postinoculation, the release of proinflammatory cytokines caused effective recruitment of neutrophils to the airway. Neutrophil influx, loss of alveolar architecture, and consolidated pneumonia were observed histologically 6 h postinoculation. Bronchoalveolar lavage fluids from mice inoculated with phosphate-buffered saline (PBS) or S. aureus were depleted of overabundant proteins and subjected to strong cation exchange fractionation followed by liquid chromatography and tandem mass spectrometry to identify the proteins present in the airway. No significant changes in response to PBS inoculation or 30 min following S. aureus inoculation were observed. However, a dramatic increase in extracellular proteins was observed 6 h postinoculation with S. aureus, with the increase dominated by inflammatory and coagulation proteins. The data presented here provide a comprehensive evaluation of the rapid and vigorous innate immune response mounted in the host airway during the earliest stages of S. aureus pneumonia.Staphylococcus aureus is a leading cause of hospital-acquired and health care-associated pneumonia and may be increasing in importance as a cause of severe community-acquired pneumonia. In the inpatient setting, it is the most common grampositive bacterium implicated in cases of ventilator-associated and hospital-acquired pneumonia (1, 9, 31). In addition, S. aureus is a frequent cause of health care-associated pneumonia occurring in residents of long-term-care facilities, individuals recently discharged from acute-care hospitals, and patients receiving outpatient treatment at hospitals and dialysis centers (1, 27, 30). A steady increase in the isolation of methicillinresistant strains of S. aureus from patients with hospital-acquired pneumonia and, more recently, community-acquired pneumonia underscores the importance of identifying host and bacterial factors that facilitate the progression of staphylococcal pneumonia.Mice have been used extensively to study pneumonia caused by a variety of bacteria (2,6,26,35,36,45,55,63,64). Murine models of airborne infection with S. aureus have been useful in characterizing host responses during the first 4 to 8 h of lung infection but do not mimic the natural route of infection and result in self-limited disease, even in immunocompromised animals (28,53,56). In these studies, proinflammatory cytokines and chemokines were released and neutrophils ...

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“…hereditary SP-B deficiency [5] or different secondary changes, i.e. with different disease status [17] or lung injury [18]. There have been estimates that at least 25% of the normal SP-B level has to be present, in the alveolar space, in order to avoid clinical symtoms [19].…”

Section: Discussionmentioning

confidence: 99%