Browning of White Adipose Tissue with Roscovitine Induces a Distinct Population of UCP1 + Adipocytes

Wang, Hong; Liu, Libin; Lin, Jean Z.; Aprahamian, Tamar; Farmer, Stephen R.

doi:10.1016/j.cmet.2016.10.005

Cited by 112 publications

(120 citation statements)

References 22 publications

(48 reference statements)

Supporting

Mentioning

112

Contrasting

Order By: Relevance

“…6A) and significantly lower plasma triglyceride concentrations (Fig. 6D), as reported previously [44,45]. In BAT, Ucp1 and mRfp1 mRNA expression levels were higher in the CL-treated group (Fig.…”

Section: Ucp1-mrfp1 Bac Tg Mice Can Be Used To Track Sequential Changsupporting

confidence: 87%

A new mouse model for noninvasive fluorescence‐based monitoring of mitochondrial UCP1 expression

et al. 2019

View full text Add to dashboard Cite

Mitochondrial uncoupling protein 1 (UCP1) is well known for its thermogenic function in brown adipose tissue (BAT). Since UCP1 expends energy on thermogenesis, UCP1 activation has been considered an approach to ameliorate obesity. As a tool for uncovering yet unknown mechanisms of UCP1 activation, we generated a transgenic mouse model in which UCP1 expression levels are reflected in fluorescence derived from monomeric red fluorescent protein 1 (mRFP1). In these UCP1‐mRFP1 BAC transgenic mice, fluorescence intensity mimics the change in UCP1 expression levels evoked through physiological or pharmacological stimulation. This transgenic mouse model will be useful in the search for bioactive compounds with the ability to induce UCP1 and for revealing undiscovered mechanisms of BAT activation.

show abstract

Section: Ucp1-mrfp1 Bac Tg Mice Can Be Used To Track Sequential Changsupporting

confidence: 87%

A new mouse model for noninvasive fluorescence‐based monitoring of mitochondrial UCP1 expression

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Beige adipocyte heterogeneity is profoundly influenced by the nature of its inducers (e.g. β3 adrenergic agonists vs PPARγ agonists) (325). The mechanism of beige fat development (also referred to as beige adipocyte recruitments or browning of WAT) remains elusive, and three models receive great attention.…”

Section: Bat and Beige Fat Developmentmentioning

confidence: 99%

Brown and Beige Adipose Tissues in Health and Disease

Rui

2017

Comprehensive Physiology

146

113

View full text Add to dashboard Cite

Brown and beige adipocytes arise from distinct developmental origins. Brown adipose tissue (BAT) develops embryonically from precursors that also give to skeletal muscle. Beige fat develops postnatally and is highly inducible. Beige fat recruitment is mediated by multiple mechanisms, including de novo beige adipogenesis and white-to-brown adipocyte transdifferentiaiton. Beige precursors reside around vasculatures, and proliferate and differentiate into beige adipocytes. PDGFRα+Ebf2+ precursors are restricted to beige lineage cells, while another PDGFRα+ subset gives rise to beige adipocytes, white adipocytes, or fibrogenic cells. White adipocytes can be reprogramed and transdifferentiated into beige adipocytes. Brown and beige adipocytes display many similar properties, including multilocular lipid droplets, dense mitochondria, and expression of UCP1. UCP1-mediated thermogenesis is a hallmark of brown/beige adipocytes, albeit UCP1-independent thermogenesis also occurs. Development, maintenance, and activation of BAT/beige fat are guided by genetic and epigenetic programs. Numerous transcriptional factors and coactivators act coordinately to promote BAT/beige fat thermogenesis. Epigenetic reprograming also influences expression of brown/beige adipocyte-selective genes. BAT/beige fat is regulated by neuronal, hormonal, and immune mechanisms. Hypothalamic thermal circuits define the temperature setpoint that guides BAT/beige fat activity. Metabolic hormones, paracrine/autocrine factors, and various immune cells also play a critical role in regulating BAT/beige fat functions. BAT and beige fat defend temperature homeostasis, and regulate body weight and glucose and lipid metabolism. Obesity is associated with brown/beige fat deficiency, and reactivation of brown/beige fat provides metabolic health benefits in some patients. Pharmacological activation of BAT/beige fat may hold promise for combating metabolic diseases.

show abstract

“…Recent studies have shown that CL316,243-induced beige adipocytes are distinct from rosiglitazone-induced brite adipocytes in gene expression patterns and origins [6,10]. Despite having those unclarified differences, both beige and brite adipocyte progenitors are presented in iWAT-SVCs, so iWAT-SVCs is a reliable cellular system to study the development of beige or brite cells in vitro [14].…”

Section: Introductionmentioning

confidence: 99%

An Additive Effect of Promoting Thermogenic Gene Expression in Mice Adipose-Derived Stromal Vascular Cells by Combination of Rosiglitazone and CL316,243

Jiang

et al. 2017

IJMS

View full text Add to dashboard Cite

It is well-documented that CL316,243 (a β3 agonist) or rosiglitazone (a PPARγ agonist) can induce white adipocyte populations to brown-like adipocytes, thus increasing energy consumption and combating obesity. However, whether there is a combined effect remains unknown. In the present study, stromal vascular cells of inguinal white adipose tissue (iWAT-SVCs for short) from mice were cultured and induced into browning by CL316,243, rosiglitazone, or both. Results showed that a combination of CL316,243 and rosiglitazone significantly upregulated the expression of the core thermogenic gene Ucp1 as well as genes related with mitochondrial function (Cidea, Cox5b, Cox7a1, Cox8b, and Cycs), compared with the treatment of CL316,243 or rosiglitazone alone. Moreover, co-treatment with rosiglitazone could reverse the downregulation of Adiponectin resulting from CL316,243 stimuli alone. Taken together, a combination of rosiglitazone and CL316,243 can produce an additive effect of promoting thermogenic gene expression and an improvement of insulin sensitivity in mouse iWAT-SVCs.

show abstract

Browning of White Adipose Tissue with Roscovitine Induces a Distinct Population of UCP1 + Adipocytes

Cited by 112 publications

References 22 publications

A new mouse model for noninvasive fluorescence‐based monitoring of mitochondrial UCP1 expression

A new mouse model for noninvasive fluorescence‐based monitoring of mitochondrial UCP1 expression

Brown and Beige Adipose Tissues in Health and Disease

An Additive Effect of Promoting Thermogenic Gene Expression in Mice Adipose-Derived Stromal Vascular Cells by Combination of Rosiglitazone and CL316,243

Contact Info

Product

Resources

About