Browning susceptibility of new hybrids of yam (Dioscorea alata) as related to their total phenolic content and their phenolic profile determined using LC-UV-MS

Rinaldo, Dominique; Sotin, Hélène; Pétro, Dalila; Lebail, Gildas; Guyot, Sylvain

doi:10.1016/j.lwt.2022.113410

Cited by 10 publications

(14 citation statements)

References 36 publications

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“…56 which is a key regulator for OB in crops through the modulation of PPO and other antioxidant enzymes. 57 How ethylene potentially modulates polyphenol profiles leading to tuber OB in D. alata 18 warrants further study.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide association studies reveal novel loci controlling tuber flesh color and oxidative browning inDioscorea alata

Dossa

Morel

Houngbo

et al. 2023

Preprint

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Background Consumers preferences for food crops are guided by quality attributes. This study aimed at deciphering the genetic basis of quality traits, especially tuber flesh color (FC) and oxidative browning (OB) in Dioscorea alata, based on the genome-wide association studies (GWAS) approach. The D. alata panel was planted at two locations in Guadeloupe. At harvest, the FC was scored visually as white, cream, or purple on longitudinally sliced mature tubers. The OB was scored visually as the presence or absence of browning after 15 minutes of exposure of the sliced samples to ambient air. Results Phenotypic characterization for FC and OB of a diverse panel of D. alata genotypes highlighted significant variation within the panel and across two locations. The genotypes within the panel displayed a weak structure and could be classified into 3 subpopulations. GWAS identified 14 and 4 significant associations for tuber FC and OB, respectively, with phenotypic variance, explained values ranging from 7.18 to 18.04%. Allele segregation analysis at the significantly associated loci highlighted the favorable alleles for the desired traits, i.e., white FC and no OB. A total of 24 putative candidate genes were identified around the significant signals. A comparative analysis with previously reported quantitative trait loci indicated that numerous genomic regions control these traits in D. alata. Conclusion Our study provides important insights into the genetic control of tuber FC and OB in D. alata. The major and stable loci can be further utilized to improve selection in breeding programs for developing new cultivars with enhanced tuber quality.

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Section: Discussionmentioning

confidence: 99%

Genome-wide association studies reveal novel loci controlling tuber flesh color and oxidative browning inDioscorea alata

Dossa

Morel

Houngbo

et al. 2023

Preprint

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“…The lyophilized powder was dissolved in ethanol aqueous (60%, v / v ) as a compound solution with a concentration of 1 mg/mL. Total phenolic content (TPC) was determined via the Folin–Ciocalteu method [ 18 , 19 ]. A standard curve was established by preparing a concentration gradient of gallic acid, and results were expressed as gallic acid equivalents per gram VWS (mg GE/g).…”

Section: Methodsmentioning

confidence: 99%

Antioxidant Activity of Vitis davidii Foex Seed and Its Effects on Gut Microbiota during Colonic Fermentation after In Vitro Simulated Digestion

Hou

Tang

et al. 2022

Foods

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Vitis davidii Foex whole seed (VWS) is a by-product during the processing of grape products, which is rich in bioactive compounds that have great potential in the food industry. In this study, the bioactive compounds and antioxidant activity of VWS were determined, and their dynamic changes during in vitro colonic fermentation were also investigated after VWS subjected to in vitro simulated digestion. Results showed that VWS were rich in polyphenols (23.67 ± 0.52 mg GAE/g), flavonoids (13.13 ± 1.22 mg RE/g), and proanthocyanidins (8.36 ± 0.14 mg CE/g). It also had good DPPH and ABTS radical scavenging activity, which reached 82.10% and 76.10% at 1000 μg/mL. The alteration trend of the antioxidant activity during in vitro fermentation for 24 h was consistent with that of the content of bioactive substances, such as polyphenols, with the extension of fermentation time. The bioactive compounds and antioxidant activity showed a trend of increasing and then decreasing, reaching the highest value at 8 h. The high-throughput sequencing analysis of the regulatory effect of VWS on intestinal micro-organisms revealed that VWS influenced intestinal microbiota diversity. The relative abundance of beneficial microbiota, such as Blautia and Parabacteroides, increased by 4.1- and 1.65-fold after 24 h of fermentation compared with that of the control group. It also reduced Escherichia-Shigella by 11.23% and effectively reduced host inflammation, while increasing the contents of acetic acid, propionic acid, and other metabolites. Taken together, these results reveal the value of VWS utilization and provide new insights into the nutritional and microbiota modulation effects of VWS, which could therefore serve as a nutraceutical ingredient in health promotion.

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“…Previous reports suggested that polyphenol oxidation (PPO) contributes to OB in yam. 16,18 A recent study by Gatarira et al identified several candidate genes associated with OB on chromosome 5. 1 Contrary to their report, we did not find any significant association for OB on chromosome 5; instead, a stable signal on chromosome 2 was identified.…”

Section: Discussionmentioning

confidence: 99%

“…34 The obvious differences between previous studies and our results are probably due to the different phenotyping procedures, genotyping methods and the quantitative nature of the trait. For example, two main phenotyping approaches (chromameter measurements 18 and a visual scoring 22 ) for yam tuber FC and OB have been used in the past. However, the correlation between these methods has not been investigated properly.…”

Section: Discussionmentioning

confidence: 99%

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Genome‐wide association studies reveal novel loci controlling tuber flesh color and oxidative browning in Dioscorea alata

et al. 2023

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BACKGROUNDConsumers' preferences for food crops are guided by quality attributes. This study aimed at deciphering the genetic basis of quality traits, especially tuber flesh color (FC) and oxidative browning (OB) in Dioscorea alata, based on the genome‐wide association studies (GWAS) approach. The D. alata panel was planted at two locations in Guadeloupe. At harvest, the FC was scored visually as white, cream, or purple on longitudinally sliced mature tubers. The OB was scored visually as the presence or absence of browning after 15 min of exposure of the sliced samples to ambient air.RESULTSPhenotypic characterization for FC and OB of a diverse panel of D. alata genotypes highlighted significant variation within the panel and across two locations. The genotypes within the panel displayed a weak structure and could be classified into three subpopulations. GWAS identified 14 and 4 significant associations for tuber FC and OB, respectively, with phenotypic variance, explained values ranging from 7.18% to 18.04%. Allele segregation analysis at the significantly associated loci highlighted the favorable alleles for the desired traits, i.e., white FC and no OB. A total of 24 putative candidate genes were identified around the significant signals. A comparative analysis with previously reported quantitative trait loci indicated that numerous genomic regions control these traits in D. alata.CONCLUSIONOur study provides important insights into the genetic control of tuber FC and OB in D. alata. The major and stable loci can be further utilized to improve selection in breeding programs for developing new cultivars with enhanced tuber quality. © 2023 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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Browning susceptibility of new hybrids of yam (Dioscorea alata) as related to their total phenolic content and their phenolic profile determined using LC-UV-MS

Cited by 10 publications

References 36 publications

Genome-wide association studies reveal novel loci controlling tuber flesh color and oxidative browning inDioscorea alata

Genome-wide association studies reveal novel loci controlling tuber flesh color and oxidative browning inDioscorea alata

Antioxidant Activity of Vitis davidii Foex Seed and Its Effects on Gut Microbiota during Colonic Fermentation after In Vitro Simulated Digestion

Genome‐wide association studies reveal novel loci controlling tuber flesh color and oxidative browning in Dioscorea alata

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