Brucella canis induces canine CD4+ T cells multi-cytokine Th1/Th17 production via dendritic cell activation

Pujol, Myriam; Borie, César; Montoya, María; Ferreira, Arturo; Vernal, Rolando

doi:10.1016/j.cimid.2018.11.017

Cited by 10 publications

(6 citation statements)

References 33 publications

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“…These authors demonstrated that B. canis induces the expression of costimulatory molecules on both canine and human dendritic cells, but while infected human dendritic cells demonstrate TH1-skewed proinflammatory cytokine production, the canine cells show a mixed TH1-TH17 response (Pujol et al, 2017). This effect was also observed in CD4+ T lymphocytes stimulated by dendritic cells previously infected with B. canis (Pujol et al, 2019). These findings demonstrate that host specificity can play an important role in the immune response to Brucella spp.…”

Section: Interaction With Natural Host Cellsmentioning

confidence: 93%

“…TH17 lymphocytes are also associated with autoimmune disorders and auto-antibody formation (Cornelius and Lamarca, 2014). The TH17 component of the response in canine dendritic cells, as suggested by Pujol et al (2017), may reflect an increased susceptibility in this host and/or help to explain the incidence of osteoarticular lesions, particularly diskospondylitis, as well as antibody-mediated destruction of sperm in infected dogs (George and Carmichael, 1984;Pujol et al, 2019). Antibody-mediated attack on sperm is also a prominent feature of B. ovis infection in rams and the possible role of IL-17 remains to be explored in this species (Paolicchi et al, 2000).…”

Section: Interaction With Natural Host Cellsmentioning

confidence: 99%

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When the Going Gets Rough: The Significance of Brucella Lipopolysaccharide Phenotype in Host–Pathogen Interactions

Stranahan

Arenas-Gamboa

2021

Front. Microbiol.

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Brucella is a facultatively intracellular bacterial pathogen and the cause of worldwide zoonotic infections, infamous for its ability to evade the immune system and persist chronically within host cells. Despite the frequent association with attenuation in other Gram-negative bacteria, a rough lipopolysaccharide phenotype is retained by Brucella canis and Brucella ovis, which remain fully virulent in their natural canine and ovine hosts, respectively. While these natural rough strains lack the O-polysaccharide they, like their smooth counterparts, are able to evade and manipulate the host immune system by exhibiting low endotoxic activity, resisting destruction by complement and antimicrobial peptides, entering and trafficking within host cells along a similar pathway, and interfering with MHC-II antigen presentation. B. canis and B. ovis appear to have compensated for their roughness by alterations to their outer membrane, especially in regards to outer membrane proteins. B. canis, in particular, also shows evidence of being less proinflammatory in vivo, suggesting that the rough phenotype may be associated with an enhanced level of stealth that could allow these pathogens to persist for longer periods of time undetected. Nevertheless, much additional work is required to understand the correlates of immune protection against the natural rough Brucella spp., a critical step toward development of much-needed vaccines. This review will highlight the significance of rough lipopolysaccharide in the context of both natural disease and host–pathogen interactions with an emphasis on natural rough Brucella spp. and the implications for vaccine development.

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Section: Interaction With Natural Host Cellsmentioning

confidence: 93%

Section: Interaction With Natural Host Cellsmentioning

confidence: 99%

When the Going Gets Rough: The Significance of Brucella Lipopolysaccharide Phenotype in Host–Pathogen Interactions

Stranahan

Arenas-Gamboa

2021

Front. Microbiol.

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“…In addition, the expression of the "role of macrophages, fibroblast, and endothelial cells in rheumatoid arthritis, " "role of osteoblasts, osteoclasts, and chondrocytes in rheumatoid arthritis, " and the "neuroinflammation signaling pathway" associated with arthritis and neurological symptoms caused by the known Brucella species has been also observed (24,(30)(31)(32)(33).…”

Section: Points This Finding Is Observed Because the Expression Of Twomentioning

confidence: 99%

Identification of Dendritic Cell Maturation, TLR, and TREM1 Signaling Pathways in the Brucella canis Infected Canine Macrophage Cells, DH82, Through Transcriptomic Analysis

et al. 2021

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Research has been undertaken to understand the host immune response to Brucella canis infection because of the importance of the disease in the public health field and the clinical field. However, the previous mechanisms governing this infection have not been elucidated. Therefore, in vitro models, which mimic the in vivo infection route using a canine epithelial cell line, D17, and a canine macrophage, DH82, were established to determine these mechanisms by performing an analysis of the transcriptomes in the cells. In this study, a coculture model was constructed by using the D17 cell line and DH82 cell line in a transwell plate. Also, a single cell line culture system using DH82 was performed. After the stimulation of the cells in the two different systems infected with B. canis, the gene expression in the macrophages of the two different systems was analyzed by using RNA-sequencing (RNA-seq), and a transcriptomic analysis was performed by using the Ingenuity Pathway Analysis (IPA). Gene expression patterns were analyzed in the DH82 cell line at 2, 12, and 24 h after the stimulation with B. canis. Changes in the upregulated or downregulated genes showing 2-fold or higher were identified at each time point by comparing with the non-stimulated group. Differentially expressed genes (DEGs) between the two culture models were identified by using the IPA program. Generally, the number of genes expressed in the single cell line culture was higher than the number of genes expressed in the coculture model for all-time points. The expression levels of those genes were higher in the single cell line culture (p < 0.05). This analysis indicated that the immune response-related pathways, especially, the dendritic cell maturation, Triggering receptor expression on myeloid cells 1 (TREM1) signaling, and Toll-like receptor (TLR) signaling pathway, were significantly induced in both the culture systems with higher p-values and z-scores. An increase in the expression level of genes related to the pathways was observed over time. All pathways are commonly associated with a manifestation of pro-inflammatory cytokines and early immune responses. However, the Peroxisome proliferator-activation receptor (PPAR) signaling and Liver X Receptor/Retinoid X Receptor (LXR/RXR) signaling associated with lipid metabolism were reduced. These results indicate that early immune responses might be highly activated in B. canis infection. Therefore, these results might suggest clues to reveal the early immune response of the canine to B. canis infection, particularly TLR signaling.

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“…Es muy probable que la infección por B. canis en los seres humanos se encuentre subdiagnosticada debido a la sintomatología inespecífica (3,4,13), y a que la bacteria no se puede identificar mediante las pruebas serológicas empleadas de rutina para el diagnóstico de otras especies del género (13). Aunque todavía se consideran poco frecuentes, un número creciente de estudios en la última década ha documentado infecciones con B. canis como causa de endocarditis, peritonitis y otros cuadros clínicos (4).…”

Section: Colombiaunclassified

“…La bacteria Brucella canis es considerada el principal agente causal de la brucelosis canina (1)(2)(3), aunque otras especies del género, como B. abortus, B. suis y B. melitensis, pueden infectar de forma transitoria a los perros (4). El cuadro clínico en el perro es variable y se encuentran desde casos asintomáticos (1,5,6), hasta abortos, orquitis, epididimitis, prostatitis (2,7), discoespondilitis (8), endoftalmitis (9) o linfadenomegalia (10).…”

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Seroprevalencia de Brucella canis en perros de un refugio para animales de compañía en Bogotá, Colombia

et al. 2021

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Introducción. El riesgo de infección con Brucella canis en humanos y perros aumenta con la exposición constante a perros portadores asintomáticos. En Colombia hay evidencia de infección con B. canis en personas que conviven con perros. Una preocupación adicional en Bogotá es la falta de información actualizada sobre la prevalencia de la infección en perros destinados a programas de adopción.Objetivo. Establecer la seroprevalencia de la infección por B. canis en perros de un refugio para animales de compañía destinados a la adopción en Bogotá.Materiales y métodos. Se hizo un estudio descriptivo de corte transversal en un refugio ara animales de Bogotá. Se detectaron anticuerpos contra B. canis en el suero de 51 perros (28 hembras y 23 machos) mediante una prueba inmunocromatográfica de flujo lateral. Asimismo, los individuos positivos se analizaron con PCR para la detección del ADN de Brucella spp.Resultado. La seroprevalencia de B. canis fue del 1,96 % (1/51). El perro seropositivo correspondió a una hembra asintomática de tres años de edad en la cual no se detectó ADN bacteriano en sangre mediante la PCR.Conclusiones. La seroprevalencia representada por un solo perro con IgG anti-B. canis puede considerarse un riesgo potencial para las poblaciones de perros y humanos, ya que podría tratarse de un animal con infección persistente capaz de diseminar la bacteria.

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Brucella canis induces canine CD4+ T cells multi-cytokine Th1/Th17 production via dendritic cell activation

Cited by 10 publications

References 33 publications

When the Going Gets Rough: The Significance of Brucella Lipopolysaccharide Phenotype in Host–Pathogen Interactions

When the Going Gets Rough: The Significance of Brucella Lipopolysaccharide Phenotype in Host–Pathogen Interactions

Identification of Dendritic Cell Maturation, TLR, and TREM1 Signaling Pathways in the Brucella canis Infected Canine Macrophage Cells, DH82, Through Transcriptomic Analysis

Seroprevalencia de Brucella canis en perros de un refugio para animales de compañía en Bogotá, Colombia

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