Brucella inopinata sp. nov., isolated from a breast implant infection

Scholz, Holger C.; Nöckler, Karsten; Göllner, Cornelia; Bahn, Peter; Vergnaud, Gilles; Tomaso, Herbert; Dahouk, Sascha Al; Kämpfer, Peter; Cloeckaert, Axel; Maquart, Marianne; Zygmunt, Michel S.; Whatmore, Adrian M.; Pfeffer, Martin; Huber, Birgit; Busse, Hans‐Jürgen; De, Barun K.

doi:10.1099/ijs.0.011148-0

Cited by 278 publications

(222 citation statements)

References 26 publications

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“…Bacteria of the genus Brucella cause brucellosis, which is recognized as a zoonotic disease of global importance (Scholz et al 2010). Brucellosis is transmitted via direct contact with animals and/or their secretions or by consumption of milk and dairy products, which has a major economic affect because the infection causes abortions and stillbirths and reduces fertility in herds (Díaz Aparicio 2013).…”

Section: Introductionmentioning

confidence: 99%

Brucella suis vaccine strain S2-infected immortalized caprine endometrial epithelial cell lines induce non-apoptotic ER-stress

Wang

Lin

Yin

et al. 2015

Cell Stress and Chaperones

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Brucella, which is regarded as an intracellular pathogen responsible for a zoonotic disease called brucellosis, survives and proliferates within several types of phagocytic and non-phagocytic cells. Brucella infects not only their preferred hosts but also other domestic and wild animal species, inducing abortion and infertility. Therefore, the interaction between uterine cells and Brucella is important for understanding the pathogenesis of this disease. In this study, we describe the Brucella suis vaccine strain S2 (B.suis.S2) infection and replication in the immortalized caprine endometrial epithelial cell line hTERT-EECs and the induced cellular and molecular response modulation in vitro. We found that B.suis S2 was able to infect and replicate to high titers and inhibit the proliferation of EECs and induce non-apoptotic pathways, as determined by B.suis.S2 detection using MTT and acridine orange/ethidium bromide (AO/EB) staining and flow cytometry. We explored the evidence of non-apoptotic pathways using real-time quantitative RT-PCR and by western blot analysis. Finally, we discovered the over-expression of GRP78, ATF4, ATF6, PERK, eIF2α, CHOP, and cytochrome c (Cyt-c) but not IRE1, xbp-1, and caspase-3 in B.suis.S2 (HK)-attacked and B.suis.S2-infected cells, suggesting that the molecular mechanism of ER stress sensor activation by B.suis.S2 is basically concomitant with that by B.suis.S2 (HK) and that ER stress, especially the PERK pathway, plays an important role in the process of B.suis.S2 infecting EEC, which may, in part, explain the role of the uterus in the pathogenesis of B.suis.S2.

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Section: Introductionmentioning

confidence: 99%

Brucella suis vaccine strain S2-infected immortalized caprine endometrial epithelial cell lines induce non-apoptotic ER-stress

Wang

Lin

Yin

et al. 2015

Cell Stress and Chaperones

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“…Novel isolates from human breast implant and lung infections (strains BO1 and BO2) and from baboons that had delivered stillborn offspring have also been described, although the natural reservoir of these isolates remains unclear. While only two isolates of each new type have been described, they have been formally published as the tenth and eleventh Brucella species, B. inopinata and B. papionis respectively (Scholz et al, 2010;Whatmore et al, 2014). Finally, strains isolated from rodents, foxes and frogs were characterised as atypical Brucella strains distinct from the currently described species.…”

Section: Description Of the Disease: Brucellosis Is The Generic Namementioning

confidence: 99%

Isolation of Brucella pinnipedialis from Grey Seals (Halichoerus grypus) in the Baltic Sea

Hirvelä-Koski

Nylund

Skrzypczak

et al. 2017

Journal of Wildlife Diseases

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Description of the disease: Brucellosis is the generic name used for the animal and human infections caused by several species of the genusCausal pathogens: Genetic and immunological evidence indicates that all members of the Brucella genus are closely related. Nevertheless, based on relevant differences in host preference and epidemiology displayed by the major variants, as well as molecular evidence of genomic variation, the International Committee on Systematics of Prokaryotes, Subcommittee on the Taxonomy of Brucella took a clear position in 2005 on a return to pre-1986 Brucella taxonomic opinion; the consequences of this statement imply the re-approval of the six classical Brucella nomenspecies with their corresponding recognised biovars. The classical names related to the six Brucella nomenspecies are validly published in the Approved Lists of Bacterial Names, 1980, and the designated type strains are attached to these validly published names: B. abortus, B. melitensis, B. suis, B. neotomae, B. ovis and B. canis 1 . The first three of these are subdivided into biovars based on cultural and serological properties (see Tables 2 and 3). Strains of Brucella have been isolated in the last decade from marine mammals but these strains cannot be ascribed to any of the above-recognised species. Investigations are continuing to establish their proper position in the taxonomy of the genus and it has been proposed that they be classified into two new species: B. ceti and B. pinnipedialis (Foster et al., 2007). A new species, named B. microti, was also isolated from the common vole (Microtus arvalis) as well as from foxes and soil in Central Europe (Scholz et al., 2008). Novel isolates from human breast implant and lung infections (strains BO1 and BO2) and from baboons that had delivered stillborn offspring have also been described, although the natural reservoir of these isolates remains unclear. While only two isolates of each new type have been described, they have been formally published as the tenth and eleventh Brucella species, B. inopinata and B. papionis respectively (Scholz et al., 2010;Whatmore et al., 2014). Finally, strains isolated from rodents, foxes and frogs were characterised as atypical Brucella strains distinct from the currently described species. They have not yet been approved as new Brucella species.Brucella is a member of the Brucellaceae family, in the order Rhizobiales, class Alphaproteobacteria. It shows close genetic relatedness to some plant pathogens and symbionts of the genera Agrobacterium and Rhizobium, as well as animal pathogens (Bartonella) and opportunistic or soil bacteria (Ochrobactrum).Infection with Brucella in cattle is usually caused by biovars (bv.) of Brucella abortus.

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“…Zoonotic potential is classified as pathogenicity and virulence in human hosts. Original citation indicates the original publication where the species was characterized.SpeciesNatural hostZoonotic Potential [8]Original Citation B. melitensis Sheep, goats, and camelsYes – High[5] B. abortus Cattle, elk, and bisonYes – High[6] B. suis Pigs, hare, reindeer/caribouYes – High[122] B. canis Dogs (domestic and wild)Yes – Moderate[123] B. ovis SheepNo reported infections[124] B. neotomae Desert wood ratsNo reported infections[125] B. ceti CetaceansYes – Low[126] B. pinnipedialis PinnipedsYes – Low B. microti Red foxes and common volesNo reported infections[127] B. inopinata UnknownYes – High[2,128] B. papionis Non-Human PrimatesNo reported infections[129,130] B. vulpis Red foxNo reported infections[131,132] Brucella NFXXXX Australian ratNo reported infections[133,134]…”

Section: Introductionmentioning

confidence: 99%

Comparisons of brucellosis between human and veterinary medicine

Hull

Schumaker

2018

Infection Ecology & Epidemiology

151

129

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Brucellosis is the world’s most widespread zoonosis, but also ranks as one of the seven most neglected diseases, according to the World Health Organization. Additionally, it is recognized as the world’s most common laboratory-acquired infection. There are a reported 500,000 incident cases of human brucellosis per year. However, true incidence is estimated to be 5,000,000 to 12,500,000 cases annually. Once diagnosed, focus is directed at treating individual patients with antibiotic regimes, yet overall neglecting the animal reservoir of disease. Countries with the highest incidence of human brucellosis are Syria (1,603.4 cases per 1,000,000 individuals), Mongolia (391.0), and Tajikistan (211.9). Surveillance on animal populations is lacking in many developed and developing countries. According to the World Animal Health Information Database, Mexico had the largest number of reported outbreaks, 5,514 in 2014. Mexico is followed by China (2,138), Greece (1,268), and Brazil (1,142). The majority of these outbreaks is Brucella abortus, the etiologic agent of bovine brucellosis. Brucellosis is an ancient disease that still plagues the world. There are still knowledge gaps and a need for better diagnostics and vaccines to make inroads towards control and eradication.

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Brucella inopinata sp. nov., isolated from a breast implant infection

Cited by 278 publications

References 26 publications

Brucella suis vaccine strain S2-infected immortalized caprine endometrial epithelial cell lines induce non-apoptotic ER-stress

Brucella suis vaccine strain S2-infected immortalized caprine endometrial epithelial cell lines induce non-apoptotic ER-stress

Isolation of Brucella pinnipedialis from Grey Seals (Halichoerus grypus) in the Baltic Sea

Comparisons of brucellosis between human and veterinary medicine

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