Brucella lipopolysaccharides and polysaccharides

O-chain expression in the rough Brucella melitensis strain B115: induction of O-polysaccharide-specific monoclonal antibodies and intracellular localization demonstrated by immunoelectron microscopy

“…The present study reports the 0-chain expression and its localization in the B. melitensis B115 rough strain which is commonly used for cytoplasmic antigen extraction (Jones et al, 1973;Zygmunt et al, 1990) and for polysaccharide B extraction (Diaz et al, 1972(Diaz et al, , 1983(Diaz et al, , 1979Moreno et al, 1981). The interest of using B. melitensis Bll5 was that it was believed to be devoid of S-LPS or 0-chain since this strain is considered as a rough strain by classical criteria (Alton et al, 1988).…”

“…The rough Brucella melitensis strain B 1 15, believed to be devoid of S-LPS or 0-chain contamination, has been 0001-7255 0 1992 SGM used for preparing cytoplasmic antigens (Jones et al, 1973;Zygmunt et al, 1990) to study the immune response and also for extraction by the trichloroacetic acid method (Diaz et al, 1972(Diaz et al, , 1979(Diaz et al, , 1983FernandezLago et al, 1982;Moreno et al, 1981) of a polysaccharide fraction called polysaccharide B. Interest in Brucella polysaccharide B fraction stems in large part from its reported efficacy in tests that differentiate vaccinated from naturally infected cattle (Diaz et al, 1979(Diaz et al, , 1981(Diaz et al, , 1983.…”

Bartonella and Brucella--Weapons and Strategies for Stealth Attack

“…They are pathogen-associated molecular pattern (PAMP), eliciting an immune response. However, Bartonella and Brucella LPSs have unique structural modifications that fail to display endotoxin activity (Table 1) (Moreno et al 1981;Rasool et al 1992;Matera et al 2008). Similar chemical modifications aiming at detoxifying LPSs and thus dampening the immune response are used by other Gram-negative bacteria such as Helicobacter pylori (Miller et al 2005).…”

“…Brucella O-polysaccharide inhibits T-cell activation, probably via sequestration of MHCII in large macrodomains in infected macrophages (Moreno et al 1981;Forestier et al 1999Forestier et al , 2000Barquero-Calvo et al 2007).…”

Αξιολόγηση της ορολογικής μεθόδου φθορίζουσας πόλωσης του φωτός (Fluorescence Polarization Assay - FPA) στη διάγνωση της βρουκέλλωσης του ανθρώπου

“…Επικρατεί η άποψη ότι πρόκειται για Ο αλυσίδα που δε συνδέεται με το LPS μόριο (Moreno E. et al 1979, Moreno E. et al 1987, Cloeckaert A. & Zygmunt M.S.1992.…”