Brucella suis urease encoded by ure 1 but not ure 2 is necessary for intestinal infection of BALB/c mice

Bandara, Aloka B.; Contreras, Andrea; Contreras-Rodríguez, Araceli; Martins, Ana M.; Dobrean, Victor; Poff-Reichow, Sherry; Rajasekaran, P.; Sriranganathan, Nammalwar; Schurig, Gerhardt G.; Boyle, Stephen M.

doi:10.1186/1471-2180-7-57

Cited by 70 publications

(57 citation statements)

References 39 publications

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“…2). Our data strongly suggest that urease, which has been reported to be related to resistance to gastrointestinal stress in several bacteria in the presence of urea (Bandara et al, 2007;Maroncle et al, 2006), is regulated by OmpR during acid survival in YpIII. Studies with other bacterial species have shown that urease expression is activated by H-NS, UreR, NikR and ArsR (Coker et al, 2000;Dattelbaum et al, 2003;Delany et al, 2005;Pflock et al, 2005;Pflock et al, 2006a), but to our knowledge OmpR-mediated urease expression has not been reported previously.…”

Section: Discussionsupporting

confidence: 69%

OmpR positively regulates urease expression to enhance acid survival of Yersinia pseudotuberculosis

Wang

et al. 2009

Microbiology

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Yersinia pseudotuberculosis is an enteric bacterium which must overcome the acidic stress in host organs for successful colonization, but how this bacterium survives in acidic conditions remains largely unknown. In the present study, the importance of OmpR in acid survival of Y. pseudotuberculosis YpIII was confirmed by the fact that mutation of ompR (strain ΔompR) greatly reduced cell survival at pH 4.5 or lower. To characterize the regulatory role of OmpR in this acid survival process, proteomic analysis was carried out to compare YpIII at pH 7.0 and pH 4.5 with ΔompR at pH 7.0, and urease components were revealed to be the main targets for OmpR regulation. Addition of urea to the culture medium also enhanced acid survival of YpIII but not ΔompR and urease activity was significantly induced by acid in YpIII but not in ΔompR. Each of the seven components of the YpIII urease gene cluster was fused to a lacZ reporter and their expression was dramatically decreased in a ΔompR background; this supports the notion that OmpR positively regulates urease expression. Furthermore, gel shift analysis revealed that OmpR binds to the deduced promoter regions of three polycistronic transcriptional units (ureABC, ureEF and ureGD) in the urease cluster, suggesting that the regulation of OmpR to urease components is direct. Taken together, these data strongly suggest that OmpR activates urease expression to enhance acid survival in Y. pseudotuberculosis.

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Section: Discussionsupporting

confidence: 69%

OmpR positively regulates urease expression to enhance acid survival of Yersinia pseudotuberculosis

Wang

et al. 2009

Microbiology

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“…melitensis wild type strain 16M, B. suis wild type 1330, and the urease-deficient B. suis mutant 1330Δure1K [6] were obtained from our bacterial culture collection. The attenuated ctpA mutant of B. suis (1330ΔctpA) [7] was used as a control.…”

Section: Methodsmentioning

confidence: 99%

“…The cultures were grown in 25 mL TSB at 37˚C with shaking at 180 rpm, and Klett units were recorded every three hours using a Klett-Summerson colorimeter. The specific activity of urease was determined using the extracts prepared from the strains grown in TSB and harvested during logarithmic growth, as described elsewhere [6,9].…”

Section: Methodsmentioning

confidence: 99%

Brucella melitensis Differs from B. suis in Growth and Urease Activity In-Vitro, and Infectivity in Fisher-344 Rats In-Vivo

Bandara¹,

Boyle²,

Contreras-Rodríguez³

et al. 2013

AID

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Importance of urease activity on pathogenic differences among Brucella species was evaluated. In cell-free extracts, the B. suis urease showed 12 times greater specific activity than the B. melitensis urease. When Fisher-344 rats were inoculated intraperitoneally (IP), at 1 week post-inoculation (PI), B. melitensis wild type 16 M was recovered from spleens and livers in greater numbers than B. suis wild type 1330. At 8 weeks PI, spleens were clear of B. melitensis, whereas B. suis remained. The wild type and the urease deficient strains of B. suis did not differ from each other in terms of recovery from spleen or liver. Our observations suggest that B. melitensis induces greater acute infectivity in Fisher-344 rats, whereas B. suis causes chronic infectivity; and urease activity has no influence on Brucella infection using an IP route.

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“…In order for proper assembly of the urease metallocenter, all four of the accessory proteins must be functional. Although urease has been demonstrated to be an important virulence factor in a number of bacteria, such as Helicobacter pylori (11), Yersinia enterocolitica (10), Proteus mirabilis (9), Brucella species (1,45), and Klebsiella pneumoniae (27), the role of urease in EHEC pathogenesis is not defined. It is possible that, like some other bacteria, EHEC exploits the enzyme urease as an additional factor to aid with surviving the low pH conditions as it passes through the stomach.…”

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confidence: 99%

Functional and Phylogenetic Analysis of ureD in Shiga Toxin-Producing Escherichia coli

2011

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Enterohemorrhagic Escherichia coli (EHEC) is a food-borne pathogen that can cause severe health complications and utilizes a much lower infectious dose than other E. coli pathotypes. Despite having an intact ure locus, ureDABCEFG, the majority of EHEC strains are phenotypically urease negative under tested conditions. Urease activity potentially assists with survival fitness by enhancing acid tolerance during passage through the stomach or by aiding with colonization in either human or animal reservoirs. Previously, in the EHEC O157:H7 Sakai strain, a point mutation in ureD, encoding a urease chaperone protein, was identified, resulting in a substitution of an amber stop codon for glutamine. This single nucleotide polymorphism (SNP) is observed in the majority of EHEC O157:H7 isolates and correlates with a negative urease phenotype in vitro. We demonstrate that the lack of urease activity in vitro is not solely due to the amber codon in ureD. Our analysis has identified two additional SNPs in ureD affecting amino acid positions 38 and 205, in both cases determining whether the encoded amino acid is leucine or proline. Phylogenetic analysis based on Ure protein sequences from a variety of urease-encoding bacteria demonstrates that the proline at position 38 is highly conserved among Gram-negative bacteria. Experiments reveal that the L38P substitution enhances urease enzyme activity; however, the L205P substitution does not. Multilocus sequence typing analysis for a variety of Shiga toxin-producing E. coli isolates combined with the ureD sequence reveals that except for a subset of the O157:H7 strains, neither the in vitro urease-positive phenotype nor the ureD sequence is phylogenetically restricted.

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Brucella suis urease encoded by ure 1 but not ure 2 is necessary for intestinal infection of BALB/c mice

Cited by 70 publications

References 39 publications

OmpR positively regulates urease expression to enhance acid survival of Yersinia pseudotuberculosis

OmpR positively regulates urease expression to enhance acid survival of Yersinia pseudotuberculosis

<i>Brucella melitensis</i> Differs from <i>B. suis</i> in Growth and Urease Activity <i>In-Vitro</i>, and Infectivity in Fisher-344 Rats <i>In-Vivo</i>

Functional and Phylogenetic Analysis of ureD in Shiga Toxin-Producing Escherichia coli

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Brucella suis urease encoded by ure 1 but not ure 2 is necessary for intestinal infection of BALB/c mice

Cited by 70 publications

References 39 publications

OmpR positively regulates urease expression to enhance acid survival of Yersinia pseudotuberculosis

OmpR positively regulates urease expression to enhance acid survival of Yersinia pseudotuberculosis

&lt;i&gt;Brucella melitensis&lt;/i&gt; Differs from &lt;i&gt;B. suis&lt;/i&gt; in Growth and Urease Activity &lt;i&gt;In-Vitro&lt;/i&gt;, and Infectivity in Fisher-344 Rats &lt;i&gt;In-Vivo&lt;/i&gt;

Functional and Phylogenetic Analysis of ureD in Shiga Toxin-Producing Escherichia coli

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<i>Brucella melitensis</i> Differs from <i>B. suis</i> in Growth and Urease Activity <i>In-Vitro</i>, and Infectivity in Fisher-344 Rats <i>In-Vivo</i>