Brucella vaccines in wildlife

Davis, Donald S.; Elzer, Philip H.

doi:10.1016/s0378-1135(02)00233-x

Cited by 59 publications

(54 citation statements)

References 17 publications

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“…Although currently very few vaccines for wildlife species have been licensed, work is on-going in a number of areas. Recent examples include experimental vaccination of wild white-footed mice (Peromyscus leucopus) [34], a reservoir host species of the human Lyme disease agent (Borrelia burgdorferi), American robins (Turdus migratorius) [35], an amplification host for West Nile Virus, and bison (Bison bison) and elk (Cervus elaphus) against brucellosis [36]. A more extensive review of the subject can be found in Delahay et al [37].…”

Section: Discussionmentioning

confidence: 99%

Bacillus Calmette-Guérin vaccination reduces the severity and progression of tuberculosis in badgers

et al. 2010

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Control of bovine tuberculosis (TB) in cattle has proven particularly challenging where reservoirs of infection exist in wildlife populations. In Britain and Ireland, control is hampered by a reservoir of infection in Eurasian badgers (Meles meles). Badger culling has positive and negative effects on bovine TB in cattle and is difficult, costly and controversial. Here we show that Bacillus Calmette-Guérin (BCG) vaccination of captive badgers reduced the progression, severity and excretion of Mycobacterium bovis infection after experimental challenge. In a clinical field study, BCG vaccination of free-living badgers reduced the incidence of positive serological test results by 73.8 per cent. In common with other species, BCG did not appear to prevent infection of badgers subjected to experimental challenge, but did significantly reduce the overall disease burden. BCG vaccination of badgers could comprise an important component of a comprehensive programme of measures to control bovine TB in cattle.

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Section: Discussionmentioning

confidence: 99%

Bacillus Calmette-Guérin vaccination reduces the severity and progression of tuberculosis in badgers

et al. 2010

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“…Although numerous studies evaluating brucellosis vaccines in livestock have been conducted during the past decades, only a limited number of vaccines have been studied in wildlife (Davis and Elzer, 2002;Olsen and Tatum, 2010). Additionally, the work has largely been done with live S19 and RB51 vaccines (Davis et al, 1991;Olsen et al, 1997Olsen et al, , 2003Olsen et al, , 2009, which were originally developed for livestock.…”

Section: Discussionmentioning

confidence: 99%

“…In most instances, the use of Brucella vaccines in wildlife species has been problematic. Vaccination with B. abortus strain RB51 has had little efficacy in bison (Davis and Elzer, 2002;Olsen et al, 2003). Strain 19 has been associated with chronic infections and abortions in bison and has been found to be ineffective as a calfhood vaccine for bison (Davis et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

DNA VACCINATION OF BISON TO BRUCELLAR ANTIGENS ELICITS ELEVATED ANTIBODY AND IFN-γ RESPONSES

Clapp

Walters

Thornburg

et al. 2011

Journal of Wildlife Diseases

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ABSTRACT:Brucella abortus remains a threat to the health and well-being of livestock in states bordering the Greater Yellowstone Area. During the past several years, cohabitation of infected wildlife with cattle has jeopardized the brucellosis-free status of Idaho, USA; Wyoming, USA; and Montana, USA. Current livestock B. abortus vaccines have not proven to be efficacious in bison (Bison bison) or elk (Cervus elaphus nelsoni). One problem with the lack of vaccine efficacy may stem from the failure to understand wildlife immune responses to vaccines. In an attempt to understand their immune responses, bison were vaccinated with eukaryotic DNA expression vectors encoding the Brucella periplasmic protein, bp26, and the chaperone protein, trigger factor (TF). These DNA vaccines have previously been shown to be protective against Brucella infection in mice. Bison were immunized intramuscularly at weeks 0, 2, and 4 with bp26 and TF DNA vaccines plus CpG adjuvant or empty vector (control) plus CpG. Blood samples were collected before vaccination and at 8, 10, and 12 wk after primary vaccination. The results showed that bison immunized with bp26 and TF DNA vaccines developed enhanced antibody, proliferative T cell, and interferon-gamma (IFN-c) responses upon in vitro restimulation with purified recombinant bp26 or TF antigens, unlike bison immunized with empty vector. Flow cytometric analysis revealed that the percentages of CD4 + and CD8 + T lymphocytes from the DNA-vaccinated groups were significantly greater than they were for those bison given empty vector. These data suggest that DNA vaccination of bison may elicit strong cellular immune responses and serve as an alternative for vaccination of bison for brucellosis.

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“…A sharp declined of platelet counts were recorded at 28 days after infection. The platelet counts decreased significantly (p < humans as well as domesticated animals [5] . Rats are found to be carriers of Brucella abortus in many parts of the world [6,7] .…”

Section: Hematological Findingsmentioning

confidence: 97%

Hematological Profiles During Acute and Subacute Brucellosis in Sprague-Dawley Rats Experimentally Infected with B. abortus biotype 1

Khatun

Islam

Baek

2016

International Journal of Hematology Research

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to uninfected control rats (p < 0.05).The blood film of acutely infected rats was characterized by abnormal red blood cells with infiltration of macrophages. Blood film of sub-acutely infected rats was characterized by abnormally shaped red blood cells with infiltration of lymphocytes and macrophages. Blood film of uninfected control rats showed normal shape of red blood cells with very few infiltrations by neutrophils. All of the rats infected with B. abortus during acute and subacute infection manifested splenomegaly. The highest spleen weights were recorded (2.25 ± 0.12 g) at 21 days after infection (p < 0.001). CONCLUSION: B. abortus induced hematological changes in SD rats during the acute and subacute stages of infection that could be useful for diagnosis of brucellosis in the free ranging wild rats. Key words: INTRODUCTIONBrucellosis is a zoonotic disease, caused by the several species of the genus Brucella and constitutes an important public health problem in many parts of the world [1] . The disease is transmitted to animals through ingestion of aborted materials, artificial insemination with contaminated semen [2] and natural breeding with infected bulls [3] .Humans can be infected with Brucella by contact with infected animals and drinking of unpasteurized milk and milk products [4] . ABSTRACT AIM: The aim of this study was to assess the hematological abnormalities such as anemia, leucopenia and thromocyopenia of Sprague-Dawley (SD) rats during the course of infection. Brucellosis in wildlife is the most likely source of infection for METHODS:The SD rats were experimentally infected ip with B. abortus biotype 1 with 1×109 colony forming unit (CFU)/ ml. Blood samples were collected from the rats at 0,3,7,14,21, 28, 35, 42, 60, 90 and 120 days, after infection. Hematological analysis of the blood samples were performed using an automated blood analyzer. Blood films were prepared on a microscopic slide using blood samples obtained from the uninfected control, acutely and subacutely infected SD rats. Thin blood films were stained with Wright-Giemsastain and examined under light microscope (Olympus, BX51) for observing the hematological changes at 1000X magnification. RESULTS: Hematological analysis revealed significant decrease of WBC, RBC, HGB, HCT and PLT in the infected rats, compared

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Brucella vaccines in wildlife

Cited by 59 publications

References 17 publications

Bacillus Calmette-Guérin vaccination reduces the severity and progression of tuberculosis in badgers

Bacillus Calmette-Guérin vaccination reduces the severity and progression of tuberculosis in badgers

DNA VACCINATION OF BISON TO BRUCELLAR ANTIGENS ELICITS ELEVATED ANTIBODY AND IFN-γ RESPONSES

Hematological Profiles During Acute and Subacute Brucellosis in Sprague-Dawley Rats Experimentally Infected with B. abortus biotype 1

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