Brucellosis of the European Brown Hare (Lepus europaeus)

Gyuranecz, Miklós; Erdélyi, Károly; Makrai, László; Fodor, László; Szépe, B.; Mészáros, A Ráczné; Dán, Ádám; Dencső, László; Fassang, E; Szeredi, Levente

doi:10.1016/j.jcpa.2010.11.013

Cited by 18 publications

(11 citation statements)

References 7 publications

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“…These results are in agreement with previous studies carried out on wild boar and hares in Europe [2-9,31]. Yet the isolation of B. suis biovar 3 from pigs, wild boar, and horses in Croatia shows the emergence of zoonotic biovars in Europe [14,16].…”

Section: Discussionsupporting

confidence: 92%

A serological and bacteriological survey of brucellosis in wild boar (Sus scrofa) in Belgium

et al. 2012

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BackgroundBrucellosis is frequently reported among wild boar populations in Europe. The aim of the study was to assess the epidemiological situation in Belgium, regarding the steady increase of wild boar populations over the last decades. Several serological tests were used and compared with culture and IS711 polymerase chain reaction (PCR), to determine the most suitable combination of diagnostic tools for conducting a successful prevalence study in wildlife.ResultsAn indirect enzyme-linked immunosorbent assay (iELISA) was used on 1168 sera from hunter-killed wild boar sampled between 2003 and 2007 in 4 natural regions of southern Belgium. Results gave an apparent prevalence of 54.88% (95% CI 52.03-57.73). Prevalence was significantly affected by age and by the year of study, but not by sex nor by the region of sampling. The relative sensitivities of the complement fixation test (CFT), the Rose Bengal test (RBT), and the slow agglutination test (SAT) versus the iELISA differed widely between tests, reaching 62.67%, 46.68%, and 34.77%, respectively. The relative specificities of the CFT, RBT and SAT versus the iELISA were respectively 99.01%, 92.49%, and 99.1%. From seropositive animals (iELISA), 9% were positive by culture and 24% by PCR when testing spleen and/or tonsils. Sensitivity of the PCR was higher on tonsils than on spleen. All bacterial isolates were identified as Brucella suis biovar 2.ConclusionsBrucellosis is widespread among wild boar in southern Belgium, with seroprevalences having increased over ten years, and constitutes a growing risk of spillback to outdoor-farmed pig herds. The iELISA showed a better sensitivity than the CFT, RBT and SAT. Serological tests must be associated with direct diagnosis and PCR proved more sensitive than culture under wildlife sampling conditions. Spleen and tonsils are lymphoid tissues usually sampled in multi-disease monitoring programs. They remain top-grade organs for direct diagnosis of brucellosis, with a preference for tonsils.

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Section: Discussionsupporting

confidence: 92%

A serological and bacteriological survey of brucellosis in wild boar (Sus scrofa) in Belgium

et al. 2012

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“…Reservoirs other than pigs may play a role in the epidemiology of human yersiniosis (18). The evidence suggests that wild boars and hares may play a role as reservoirs for biotype 2 (serotypes O:9 and O:5, 27) (1,4,16). Until now, there have been no studies on the virulence markers of Y. enterocolitica O:9 isolated from pigs in which initially positive serological reactions for brucellosis were reported.…”

Section: Discussionmentioning

confidence: 99%

“…The disease is commonly occurring; however in Europe, where the causative agent is B. suis biovar 2, its prevalence is low (1). The potential reservoirs of this biovar are wild boars and hares (1,4). In Poland, the last outbreaks of porcine brucellosis were recorded in 1994 and 1999.…”

Section: Introductionmentioning

confidence: 99%

“…In elderly people, systemic forms of the disease are often diagnosed (18). The Y. enterocolitica species could be divided into six biotypes, which are based on biochemical properties: 1A, which is generally considered as non-pathogenic, and pathogenic 1B, 2, 3, 4, and 5 biotypes (22 (4,17,18). The potential pathogenicity can be determined using PCR methods.…”

Section: Introductionmentioning

confidence: 99%

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Genotypic virulence markers of Yersinia enterocolitica O:9 isolated from pigs and wild boars serologically positive and negative for brucellosis

Weiner

Kubajka

Szulowski

et al. 2014

Bulletin of the Veterinary Institute in Pulawy

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The paper concerns molecular study on pathogenicity markers of fourteen Y. enterocolitica O:9 isolated from pigs in which initially positive serological reactions for brucellosis were observed (n = 41), healthy pigs, which were brucellosis-negative (n = 258), and wild boars serologically negative for brucellosis (n = 209). PCR identification proved that all isolates were ail, ystA-and myfA-positive. The plasmid encoding yadA marker was detected in nine isolates that originated from pigs serologically positive or negative for brucellosis, and from one isolate of wild boar origin. Furthermore, none of the examined isolates was ystB-positive. Results of the investigations indicate that the Y. enterocolitica O:9 isolates from pigs or wild boars, regardless of whether they were serologically positive or negative for brucellosis, may also be potentially pathogenic for humans, due to the presence of chromosomal and plasmid encoded molecular markers.

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“…Recent cases of human brucellosis in Australia and the U.S. have been attributed to contact with B. suis when dressing, transporting, and/or cooking the infected animals, demonstrating the importance of these animals from a public health standpoint (Starnes et al, 2004;CDC, 2009;Irwin et al, 2010). In Europe, B. suis biovar 2 has historically been isolated from European wild boar, although European hares (Lepus europaeus) are also infected (Sterba, 1983;Gyuranecz et al, 2011). As is the case with feral swine in the U.S., the range of wild boar in Europe has expanded due to stocking for hunting (Acevedo et al, 2006).…”

Section: Brucellosis In Feral Swine and Wild Boarmentioning

confidence: 99%

Feral swine brucellosis in the United States and prospective genomic techniques for disease epidemiology

Leiser

Corn

Schmit

et al. 2013

Veterinary Microbiology

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Brucellosis of the European Brown Hare (Lepus europaeus)

Cited by 18 publications

References 7 publications

A serological and bacteriological survey of brucellosis in wild boar (Sus scrofa) in Belgium

A serological and bacteriological survey of brucellosis in wild boar (Sus scrofa) in Belgium

Genotypic virulence markers of Yersinia enterocolitica O:9 isolated from pigs and wild boars serologically positive and negative for brucellosis

Feral swine brucellosis in the United States and prospective genomic techniques for disease epidemiology

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