Brucelosis, una zoonosis presente en la población: estudio de series de tiempo en México

Abstract

Objetivo. Determinar el comportamiento de la incidencia de brucelosis humana en México durante el periodo 2000-2011 y su relación con la brucelosis en rumiantes domésticos. Material y métodos. En estudio ecológico de series de tiempo se analizaron, mediante regresión Poisson multinivel múltiple, la incidencia de brucelosis humanas durante 2000- 2011 y la incidencia en rumiantes, y su relación con índice de desarrollo humano. Resultados. La alta incidencia de brucelosis bovina aumenta 15% la incidencia de brucelosis humana. La alta incidencia en caprinos aumenta 33% la incidencia de brucelosis humana. La presencia de casos nuevos de brucelosis ovina aumenta 13% la incidencia de brucelosis humana. Conclusiones. La brucelosis es un problema de salud pública; la presencia de la brucelosis en los rumiantes domésticos y el nivel de control de la enfermedad en ellos afecta la presencia de la enfermedad en humanos.

Diagnosis of Caprine Brucellosis by Serology and Multiple PCR

“…In the same manner, Irajian et al analyzed 68 isolations by PCR from humans as well as from animals, among which B. melitensis predominated in 36 isolates, two of B. abortus and one of B. suis of the animal specimen, and 24 isolates of B. melitensis and six of B. abortus of the human specimen [8]. In this regard, it was documented that B. melitensis presents very severe clinical conditions in humans [2].…”

“…The sampling was consecutive in gestating goats 12 months of age with and without brucellosis. We collected approximately 10 mL of blood per goat; sampling was conducted in tubes 2,3 , and immediately afterward, the tubes were transported in coolers for their processing. The serum was separated from the blood by centrifugation 4 at 3,000 rpm during 10 min.…”

“…We placed 1.5 mL of culture in an Eppendorf tube and centrifuged this for 2 min at 13,000 rpm. The supernatant was eliminated and the cellular pellet was resuspended in 1 mL of lysis 2 BD Vacutainer® Suero/SERUM 13 × 100 mm. 3 buffer (EDTA 50 mM, NaCl 100 mM, pH 7.5), was mixed in a vortex until resuspension, and was centrifuged for 2 min at 13,000 rpm to recover the bacterial pellet again and the supernatant was eliminated.…”

“…Its incidence ranges from between 1.3 and 70.0 cases per 100,000 inhabitants, differences due to the characteristics of each nation. Mexico is one of the countries with the greatest incidence of human brucellosis in Latin America, causing economic loses generated in domestic cattle-raising and its impact on public health [2]. The highest brucellosis incidence rates were found in bovines, followed by caprines and ovines.…”

Comparative Field Trial Effect of Brucella spp. Vaccines on Seroconversion in Goats and Their Possible Implications to Control Programs

“…The disease often occurs in cattle, sheep, and goat production units; the latter is the most important given its potential role in conveying disease to human. Because brucellosis is a public health problem, its presence and disease control strategies implemented in domestic ruminants affect the occurrence of disease in humans [8,9]. In small ruminants, the disease is clinically characterized by a decrease in milk production, abortion, loss of weight, fetal death, placental retention, weak offspring, and acute orchitis.…”