BS5.3 - Piezoelectric quartz crystal aptamer biosensor for detection and quantification of SARS CoV helicase protein

Albano, Dharmatov Rahula; Shum, KT; Tanner, JA; Fung, YS

doi:10.5162/imcs2018/bs5.3

Cited by 10 publications

(3 citation statements)

References 2 publications

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“…Piezoelectric quartz crystal microbalance (QCM) nanobiosensors have achieved recognition for the medical applications due to their simplicity, label-free testing, flat surfaces and real-time responses [ 158 , 159 , 160 ]. According to Albano et al, detection of protein biomarkers at the pg/mL level is possible by analyzing the effect of paramagnetic nanoparticles using a piezoelectric quartz crystal nanobiosensor [ 161 ]. Particularly, a piezoelectric immunosensor can be identified as stable, effective and fast for SARS-CoV detection ( Figure 11 ) [ 162 ].…”

Section: Discussionmentioning

confidence: 99%

Biosensors as Nano-Analytical Tools for COVID-19 Detection

Pradhan

Lahare

Sinha

et al. 2021

Sensors

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Selective, sensitive and affordable techniques to detect disease and underlying health issues have been developed recently. Biosensors as nanoanalytical tools have taken a front seat in this context. Nanotechnology-enabled progress in the health sector has aided in disease and pandemic management at a very early stage efficiently. This report reflects the state-of-the-art of nanobiosensor-based virus detection technology in terms of their detection methods, targets, limits of detection, range, sensitivity, assay time, etc. The article effectively summarizes the challenges with traditional technologies and newly emerging biosensors, including the nanotechnology-based detection kit for COVID-19; optically enhanced technology; and electrochemical, smart and wearable enabled nanobiosensors. The less explored but crucial piezoelectric nanobiosensor and the reverse transcription-loop mediated isothermal amplification (RT-LAMP)-based biosensor are also discussed here. The article could be of significance to researchers and doctors dedicated to developing potent, versatile biosensors for the rapid identification of COVID-19. This kind of report is needed for selecting suitable treatments and to avert epidemics.

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Section: Discussionmentioning

confidence: 99%

Biosensors as Nano-Analytical Tools for COVID-19 Detection

Pradhan

Lahare

Sinha

et al. 2021

Sensors

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“…Based on the measurement, a linear correlation was found in the range of 10 -6 -10 -2 μg ml −1 (%I loss = 6.7883 log CoV-2 antigen)+63.1961; (R2 = 0.9588)) as shown in the inset in figure 8(a). The limit of detection (LOD) was 2 fg ml -1 , calculated using the equation yb + 3Stdb, whereas yb was the mean fluorescence intensity loss measured for the blank control and Stdb was the associated standard deviation [45].…”

Section: Analytical Performance Of Fe-fsnp As Biosensormentioning

confidence: 99%

An ultra-sensitive SARS-CoV-2 antigen optical biosensor based on angiotensin converting enzyme 2 (ACE-2) functionalized magnetic-fluorescent silica nanoparticles

Maulana,

Raissa,

Nurrudin

et al. 2024

Nanotechnology

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This work reports on the design and synthesis of an ACE-2 functionalized magnetic fluorescent silica nanoparticles (Fe-FSNP) as a biosensing platform to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antigen. Iron oxide (Fe3O4) nanoparticles were synthesized via ultrasonic-assisted coprecipitation and then coated with fluorescent silica nanoparticles (FSNP) through the sol-gel method forming the Fe-FSNP samples. Silica obtained from local geothermal powerplant was used in this work and Rhodamine B was chosen as the incorporated fluorescent dye, hence this reports for the first time ACE-2 was immobilized on the natural silica surface. The Fe-FSNP nanoparticle consists of a 18-25 nm magnetic core and a silica shell with a thickness of 30 nm as confirmed from the transmission electron microscopy (TEM) image. Successful surface functionalization of the Fe-FSNP with ACE-2 as bioreceptor was conducted through hydrosylilation reaction and confirmed through the fourier transform infrared (FTIR) spectroscopy. The detection of SARS-Cov-2 antigen by Fe-FSNP/ACE2 was measured through the change in its maximum fluorescence intensity at 588 nm where fluorescence- quenching had occurred. The biosensing platform showed a rapid response at 30 minutes with a linear range of 10-6 to 10-2 μg/mL. The magnetic-fluorescent properties of the nanoparticle enables an ultra-sensitive detection of SARS-Cov-2 antigen with the limit of detection (LoD) as low as 2 fg/mL.

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“…The duo developed a piezoelectric sensor using a lead zirconate titanate piezoelectric disk and successfully performed a label-free detection of the influenza virus. In another study, it was observed that a piezoelectric sensor coated with aptamer could detect the SCV helicase protein derived from the SARS CoV [101] . The sensor could perform the detection in a minute and showed a LOD of 3.5 ng/mL.…”

Section: Types Of Sensors For Respiratory Viral Detectionmentioning

confidence: 99%

Nanomaterials-Based Sensors for Respiratory Viral Detection: A Review

et al. 2021

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Contagious diseases are the principal cause of mortality, particularly respiratory viruses, a real menace for public health and economic development worldwide. Therefore, timely diagnosis and treatments are the only lifesaving strategy to overcome any epidemic and particularly the ongoing prevailing pandemic COVID-19 caused by SARS-CoV-2. A rapid identification, point of care, portable, highly sensitive, stable, and inexpensive device is the needed which is exceptionally satisfied by sensors. Consequently, the researchers have directed their attention to employing sensors targeting multiple analyses of pathogenic detections across the world. Nanostructured materials (nanoparticles, nanowires, nanobundles, etc.), owing to their unique characteristics such as large surface-to-volume ratio and nanoscale interactions, are widely employed to fabricate facile sensors to meet all the immediate emerging challenges and threats. This review is anticipated to foster researchers in developing advanced nanomaterials-based sensors for the increasing number of COVID-19 cases across the globe. The mechanism of respiratory viral detection by nanomaterials-based sensors has been reported. Moreover, the advantages, disadvantages, and their comparison with conventional sensors are summarized. Furthermore, we have highlighted the challenges and future potential of these sensors for achieving efficient and rapid detection.

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BS5.3 - Piezoelectric quartz crystal aptamer biosensor for detection and quantification of SARS CoV helicase protein

Cited by 10 publications

References 2 publications

Biosensors as Nano-Analytical Tools for COVID-19 Detection

Biosensors as Nano-Analytical Tools for COVID-19 Detection

An ultra-sensitive SARS-CoV-2 antigen optical biosensor based on angiotensin converting enzyme 2 (ACE-2) functionalized magnetic-fluorescent silica nanoparticles

Nanomaterials-Based Sensors for Respiratory Viral Detection: A Review

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