BSA as additive: A simple strategy for practical applications of PNA in bioanalysis

Lee, Jieon; Park, Il Soo; Kim, Henna; Woo, Jae Sung; Choi, Byung-Seok; Min, Dal Hee

doi:10.1016/j.bios.2015.02.030

Cited by 25 publications

(15 citation statements)

References 38 publications

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“…In our experiment, we first immersed the sensing surface with capture antibodies. To block non-specific binding in the sensing surface, we flowed BSA molecules, which is widely used as blocking agent [64][65][66], into the microfluidic chamber in advance to block the unbound sites in the sensing substrate. Then an antigen-containing solution was injected into the microfluidic chamber.…”

Section: Sub-attomole Cancer Biomarker Detectionmentioning

confidence: 99%

Targeted Sub-Attomole Cancer Biomarker Detection Based on Phase Singularity 2D Nanomaterial-Enhanced Plasmonic Biosensor

et al. 2021

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Highlights A zero-reflection-induced phase singularity is achieved through precisely controlling the resonance characteristics using two-dimensional nanomaterials. An atomically thin nano-layer having a high absorption coefficient is exploited to enhance the zero-reflection dip, which has led to the subsequent phase singularity and thus a giant lateral position shift. We have improved the detection limit of low molecular weight molecules by more than three orders of magnitude compared to current state-of-art nanomaterial-enhanced plasmonic sensors. Abstract Detection of small cancer biomarkers with low molecular weight and a low concentration range has always been challenging yet urgent in many clinical applications such as diagnosing early-stage cancer, monitoring treatment and detecting relapse. Here, a highly enhanced plasmonic biosensor that can overcome this challenge is developed using atomically thin two-dimensional phase change nanomaterial. By precisely engineering the configuration with atomically thin materials, the phase singularity has been successfully achieved with a significantly enhanced lateral position shift effect. Based on our knowledge, it is the first experimental demonstration of a lateral position signal change > 340 μm at a sensing interface from all optical techniques. With this enhanced plasmonic effect, the detection limit has been experimentally demonstrated to be 10–15 mol L−1 for TNF-α cancer marker, which has been found in various human diseases including inflammatory diseases and different kinds of cancer. The as-reported novel integration of atomically thin Ge2Sb2Te5 with plasmonic substrate, which results in a phase singularity and thus a giant lateral position shift, enables the detection of cancer markers with low molecular weight at femtomolar level. These results will definitely hold promising potential in biomedical application and clinical diagnostics.

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Section: Sub-attomole Cancer Biomarker Detectionmentioning

confidence: 99%

Targeted Sub-Attomole Cancer Biomarker Detection Based on Phase Singularity 2D Nanomaterial-Enhanced Plasmonic Biosensor

et al. 2021

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“…The combination of PNA and GO allows the quantitative fluorometric NA sensing even in the biological samples without a wide variation of LOD, which was impossible to achieve by using natural DNA probe. To improve the performance of the PNA utilized FRET sensors, bovine serum albumin (BSA) was used as an additive for minimizing nonspecific adsorption of biomolecules to GO surface and for effectively desorbing the target/PNA duplex from GO surface while keeping the interaction between ssPNA and GO is unaffected [65].…”

Section: Fluorescent Biosensors Using Artificial Pna Probementioning

confidence: 99%

Biosensors based on graphene oxide and its biomedical application

Lee

Kim

et al. 2016

Advanced Drug Delivery Reviews

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336

173

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Graphene oxide (GO) is one of the most attributed materials for opening new possibilities in the development of next generation biosensors. Due to the coexistence of hydrophobic domain from pristine graphite structure and hydrophilic oxygen containing functional groups, GO exhibits good water dispersibility, biocompatibility, and high affinity for specific biomolecules as well as properties of graphene itself partly depending on preparation methods. These properties of GO provided a lot of opportunities for the development of novel biological sensing platforms, including biosensors based on fluorescence resonance energy transfer (FRET), laser desorption/ionization mass spectrometry (LDI-MS), surface-enhanced Raman spectroscopy (SERS), and electrochemical detection. In this review, we classify GO-based biological sensors developed so far by their signal generation strategy and provide the comprehensive overview of them. In addition, we offer insights into how the GO attributed in each sensor system and how they improved the sensing performance.

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“…BSA, having hydrophobic regions on its surface, is widely used as an additive in biological experiments to reduce the non-specific binding of other substances. 40 As shown in Fig. S11, † only a slight increase of the absorbance of Δ -1 was observed in different concentrations of BSA ranging from 0.67–6.67 μg mL –1 in PBS buffer.…”

Section: Resultsmentioning

confidence: 84%

Inhibition of the Ras/Raf interaction and repression of renal cancer xenografts in vivo by an enantiomeric iridium(iii) metal-based compound

et al. 2017

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BSA as additive: A simple strategy for practical applications of PNA in bioanalysis

Cited by 25 publications

References 38 publications

Targeted Sub-Attomole Cancer Biomarker Detection Based on Phase Singularity 2D Nanomaterial-Enhanced Plasmonic Biosensor

Targeted Sub-Attomole Cancer Biomarker Detection Based on Phase Singularity 2D Nanomaterial-Enhanced Plasmonic Biosensor

Biosensors based on graphene oxide and its biomedical application

Inhibition of the Ras/Raf interaction and repression of renal cancer xenografts in vivo by an enantiomeric iridium(iii) metal-based compound

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