Bta-miR-125a Regulates Milk-Fat Synthesis by Targeting SAA1 mRNA in Bovine Mammary Epithelial Cells

Abstract

The nutritional value of cow milk mainly depends on its fatty acid content and protein composition. The identification of genes controlling milk production traits and their regulatory mechanisms is particularly important for accelerating genetic progress in the breeding of dairy cows. On the basis of mammary gland transcriptome analyses, in this study we identified an miRNA, bta-miR-125a, that could control bovine milk-fat production by targeting the 3′ untranslated region (UTR) of the serum amyloid A-1 (SAA1) mRNA. The presence of synthetic bta-miR-125a (i.e., an miR-125a mimic) significantly down-regulated the expression of luciferase from mRNAs containing the binding sequence for bta-miR-125a in the 3′-UTR in a dual-luciferase reporter assay. Furthermore, the presence of the miR-125a mimic decreased the steady-state level of the SAA1 protein, but increased the accumulation of triglycerides and cholesterol content in bovine mammary epithelial cells (MAC-Ts). Blocking the function of bta-miR-125a using a specific inhibitor decreased the level of triglycerides and cholesterol content in the cells. These results indicate that bta-miR-125a can serve as a positive regulator of lipid synthesis in mammary epithelial cells, which acts by targeting SAA1 gene expression.

Genetics and Production of Safe, High-Quality Milk by Dairy Cattle

“…Meanwhile, based on mammary transcriptome analysis, a kind of miRNA, bta-miR-125a, has been identified as controlling bovine-milk fat production by targeting the 3 untranslated region (UTR) of serum amyloid A-3 (SAA1) mRNA. This miRNA can act as a positive regulator of lipid synthesis in mammary epithelial cells by targeting SAA1 gene expression [4]. N6-methyladenosine (m 6 A) methylation, a methylation modification found on mRNA molecules, has also been suggested to play a role in the development of lipid metabolism since it can change the mRNA levels of genes related to milk-fat metabolism.…”