bta-miR-23a involves in adipogenesis of progenitor cells derived from fetal bovine skeletal muscle

Guan, Long; Hu, Xin; Liu, Li; Xing, Yishen; Zhou, Zhengkui; Liang, Xingwei; Yang, Qiyuan; Jin, Sheng; Bao, Jinsong; Gao, Huijiang; Du, Min; Li, Junya; Zhang, Lupei

doi:10.1038/srep43716

Cited by 54 publications

(57 citation statements)

References 59 publications

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“…), miR‐23a is involved in adipogenesis (Guan et al . ) and miR‐199a‐3p affects adipocyte differentiation and fatty acid composition (Tan et al . ).…”

Section: Discussionmentioning

confidence: 99%

Comparison of microRNAs in adipose and muscle tissue from seven indigenous Chinese breeds and Yorkshire pigs

Liang

Wang

et al. 2019

Animal Genetics

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Summary Elucidation of the pig microRNAome is essential for interpreting functional elements of the genome and understanding the genetic architecture of complex traits. Here, we extracted small RNAs from skeletal muscle and adipose tissue, and we compared their expression levels between one Western breed (Yorkshire) and seven indigenous Chinese breeds. We detected the expression of 172 known porcine microRNAs (miRNAs) and 181 novel miRNAs. Differential expression analysis found 92 and 12 differentially expressed miRNAs in adipose and muscle tissue respectively. We found that different Chinese breeds shared common directional miRNA expression changes compared to Yorkshire pigs. Some miRNAs differentially expressed across multiple Chinese breeds, including ssc‐miR‐129‐5p, ssc‐miR‐30 and ssc‐miR‐150, are involved in adipose tissue function. Functional enrichment analysis revealed that the target genes of the differentially expressed miRNAs are associated mainly with signaling pathways rather than metabolic and biosynthetic processes. The miRNA–target gene and miRNA–phenotypic traits networks identified many hub miRNAs that regulate a large number of target genes or phenotypic traits. Specifically, we found that intramuscular fat content is regulated by the greatest number of miRNAs in muscle tissue. This study provides valuable new candidate miRNAs that will aid in the improvement of meat quality and production.

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“…), miR‐23a is involved in adipogenesis (Guan et al . ) and miR‐199a‐3p affects adipocyte differentiation and fatty acid composition (Tan et al . ).…”

Section: Discussionmentioning

confidence: 99%

Comparison of microRNAs in adipose and muscle tissue from seven indigenous Chinese breeds and Yorkshire pigs

Liang

Wang

et al. 2019

Animal Genetics

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“…miRNAs serve different roles (promote or suppress cancer development) in cancer cellular processes, including in endometrial cancer (2,(5)(6)(7)(8)(9)(10)(11)(12)(13). Previously, miR-23a has been linked to chemoresistance in cancer (10,12,(15)(16)(17)19), and there have been three studies investigating the association between overexpression of miR-23a in cancer and chemoresistance (15,16,19). However, thus far, only one study investigated the association between miR-23a and endometrial cancer, and indicated that miR-23a is downregulated in cancer tissue and that miR-23a may inhibit EMT in endometrial endometrioid adenocarcinoma by targeting SMAD3 (14).…”

Section: Discussionmentioning

confidence: 99%

“…Reagents for the transient transfection assays. miR-23a mimics (5'-AUC ACA UUG CCA GGG AUU UCC-3'), miR-23a mimic NC (5'-UU CUC CGA ACG UGU CAC GUTT-3'), miR-23a antisense oligonucleotide (ASO; 5'-GUG GUA AUC CCU GGC AAU GUG AU-3' and ASO-NC (5'-CAG UAC UUU UGU GUA GUA CAA-3') were obtained from Sangon Biotech Co., Ltd. (Shanghai, China) SIX1 small interfering (si)RNA and a pcDNA3.1 SIX1 overexpression plasmid were obtained from Guangzhou Ribobio Co., Ltd. (Guangzhou, China) (17,18). miR-23a mimics were transfected into Ishikawa cells, miR-23a ASO into HEC1B cells, and SIX1 siRNA and plasmid into Ishikawa and HEC1B cells.…”

Section: Methodsmentioning

confidence: 99%

“…HEC1B cells (8x10 6 ) were suspended in 0.1 ml DMEM, which was injected subcutaneously into the right flank of each mouse (20). The mice were divided into five groups and injected with control (transfected with vector), Taxol, miR-23a con, miR-23a or miR-23a+Taxol once every 3 or 4 days from day 14 (17). The final concentration of control, miR-23a con and miR-23a for each intradermal injection of HEC1B cells (0.01 mol) was 100 nM.…”

Section: Dual-luciferase Reporter Assay a Dual-glo Luciferase Assaymentioning

confidence: 99%

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MicroRNA‑23a inhibits endometrial cancer cell development by targeting SIX1

Sun

et al. 2019

Oncol Lett

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The present study focused on exploring the inhibitory mechanism of microRNA (miR)-23a in endometrial cancer. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) was used to investigate miR-23a expression in endometrial tissues and endometrial cancer cells. A colony formation assay using crystal violet staining was performed to compare cell proliferation, while wound-healing and Transwell assays were performed to compare cell migration and invasion. Subsequently, bioinformatics and a luciferase reporter gene assay were used to investigate the effect of miR-23a on sine oculis homeobox homolog 1 (SIX1) expression, and the biological function of SIX1 was analyzed. Additionally, a nude mouse tumorigenicity assay was performed to test the inhibitory effect of miR-23a and Taxol ® therapy in endometrial cancer. Finally, immunohistochemistry and RT-qPCR were used to explore the association between miR-23a and SIX1 expression in endometrial cancer tissues. miR-23a was underexpressed in endometrial cancer tissues compared with in para-carcinoma tissues, and the overexpression of miR-23a inhibited proliferation and invasion of endometrial cancer cells. Furthermore, SIX1 was demonstrated to be a downstream target of miR-23a, and miR-23a reduced SIX1 expression. Additionally, SIX1 inversely promoted cell proliferation, migration and invasion. In addition, the effects of reduced cell proliferation and increased cell invasion following miR-23a overexpression could be reversed by adding SIX1 to in vitro culture. Furthermore, the inhibitory effect of miR-23a and Taxol therapy, which reduced SIX1 expression in endometrial cancer, was demonstrated in vivo. Finally, a negative association between miR-23a and SIX1 expression was demonstrated in endometrial cancer tissues. The results of the present study revealed that miR-23a may inhibit endometrial cancer development by targeting SIX1.

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“…As a model system for validating the performance of Lipo‐mB, we monitored the expression changes in diverse miRNAs related to adipogenesis (upregulation: miR‐181a and miR‐21; downregulation: miR‐31) in culture soup during adipogenesis of bone marrow mesenchymal stem cells (BMMSCs) with Lipo‐mB ( Figure ) . The miRNA expression profile obtained using Lipo‐mB, was correlated with the adipogenesis state, and we successfully monitored adipogenesis by analyzing miRNA expression profiles in culture soup.…”

Section: Introductionmentioning

confidence: 99%

Convenient Monitoring System of Intracellular microRNA Expression during Adipogenesis via Mechanical Stimulus‐Induced Exocytosis of Lipovesicular miRNA Beacon

Han

Kang

Jang

et al. 2017

Adv Healthcare Materials

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Noninvasive investigation of microRNAs (miRNAs) expression, which is deeply related to biological phenomena such as stem cell differentiation, in culture soup is particularly useful for monitoring of stem cell differentiation without phototoxicity of living cells, especially when cell morphologies remain unchanged during differentiation. However, real-time detection of miRNA in culture soup is not recommended because of insufficient miRNA amounts in culture soup. In this study, a convenient method is introduced for real-time assessing intracellular miRNA in culture soup by using lipovesicular miRNA beacon (Lipo-mB) and mechanical stimulus-mediated exocytosis. Pipetting-harvest of culture soup induces exocytosis-secretion of fluorescence signal of Lipo-mB from cytoplasm into culture soup. To demonstrate this method, Lipo-mB is applied for monitoring of adipogenesis by analyzing the expression levels of various intracellular miRNAs, which are related to adipogenesis regulators. The fluorescence intensity profile of the culture soup is correlated with the quantitative reverse-transcription-polymerase chain reaction data and absorbance of Oil Red O staining. These results demonstrate that Lipo-mB can successfully monitor stem cell differentiation by sensing changes in miRNA expression from culture soup of living cells. Lipo-mB can be further developed as an accurate sensing system for analyzing subtle differences in genotype, even when changes in phenotype cannot be observed.

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bta-miR-23a involves in adipogenesis of progenitor cells derived from fetal bovine skeletal muscle

Cited by 54 publications

References 59 publications

Comparison of microRNAs in adipose and muscle tissue from seven indigenous Chinese breeds and Yorkshire pigs

Comparison of microRNAs in adipose and muscle tissue from seven indigenous Chinese breeds and Yorkshire pigs

MicroRNA‑23a inhibits endometrial cancer cell development by targeting SIX1

Convenient Monitoring System of Intracellular microRNA Expression during Adipogenesis via Mechanical Stimulus‐Induced Exocytosis of Lipovesicular miRNA Beacon

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