Bu-shen-zhu-yun decoction promotes synthesis and secretion of FSHβ and LHβ in anterior pituitary cells in vitro

Jiang, Xiaofei; Zhou, Huifang; Zhou, Boru; Liu, Bei; Yuan, Yi-Zhen; Shan, Jinjun; Xie, Ting

doi:10.1016/j.biopha.2018.02.003

Cited by 8 publications

(6 citation statements)

References 28 publications

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“…Accordingly, GGQLD-medicated serum inhibited the level of Ac-FOXO1 in palmitic acid-treated HepG2 cells. We also found that GGQLD counteracted the induction of FABP4, an indicator of insulin resistance [20], by palmitic acid. Therefore, our results demonstrated that, in T2DM, GGQLD enhanced the expression of SIRT1 and subsequently reduced the level of Ac-FOXO1, as did pioglitazone, in vitro and in vivo .…”

Section: Discussionmentioning

confidence: 99%

Gegen Qinlian Decoction Ameliorates Hepatic Insulin Resistance by Silent Information Regulator1 (SIRT1)-Dependent Deacetylation of Forkhead Box O1 (FOXO1)

et al. 2019

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BackgroundGegen qinlian decoction (GGQLD) is a form of traditional Chinese medicine used for hundreds of years for its efficacy in treating diabetes. However, the mechanisms underlying the therapeutic effects of GGQLD on diabetes are still not clear. We aimed to evaluate the effect of GGQLD on hepatic insulin resistance (IR) through silent information regulator1 (SIRT1)/forkhead box O1 (FOXO1) in an IR mouse model.Material/MethodsA high-fat diet (HFD) mouse model was established and GGQLD was administrated by oral gavage. Metabolic parameters were detected, including body weights, triglyceride, fasting glucose, fasting insulin and HOMA-IR index, glucose intolerance, and insulin resistance. HE-stained sections were used to observe the histopathology of liver tissue. For in vitro study, GGQLD-medicated serum was used to treat palmitic acid-stimulated HepG2 cells. The glycogen synthesis and downstream SIRT1/FOXO1 signaling pathways were examined. Specific siRNAs were used to knock down SIRT1 in HepG2 cells.ResultsGGQLD administration significantly decreased body weights, triglyceride level, fasting glucose level, fasting insulin level, and HOMA-IR index, and improved IR in HFD mice. GGQLD enhanced SIRT1 expression and suppressed the expression of Ac-FOXO1 in liver tissues. Further, GGQLD-medicated serum promoted SIRT1 upregulation and suppressed Ac-FOXO1 levels in palmitate-stimulated HepG2 cells. GGQLD-medicated serum also increased the protein expression of PPARγ and reduced the expression of FABP4 in palmitate-stimulated HepG2 cells.ConclusionsWe found that GGQLD alleviates insulin resistance through SIRT1-dependent deacetylation of FOXO1.

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Section: Discussionmentioning

confidence: 99%

Gegen Qinlian Decoction Ameliorates Hepatic Insulin Resistance by Silent Information Regulator1 (SIRT1)-Dependent Deacetylation of Forkhead Box O1 (FOXO1)

et al. 2019

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“…The theory of Chinese medicine believes that the kidney affects reproduction, which is the innate foundation and the root of vitality. Ovulation relies on several factors, such as kidney, liver, spleen, qi, blood, yin and yang [ 7 ]. A previous study of meta-analysis has represented that BSZY-D can significantly elevate the pregnancy rate to 50%, comparing to the pregnancy rate of 30% using vitro fertilization [ 11 ].…”

Section: Discussionmentioning

confidence: 99%

“…This decoction has been proved to regulate menstruation and assist pregnancy [ 5 ]. BSZY-D has been widely applied in gynecological diseases, such as luteal phase defect, anovulatory infertility, and polycystic ovarian syndrome [ 5 – 7 ]. However, the efficacy of BSZY-D in HPRL infertility remains elusive.…”

Section: Introductionmentioning

confidence: 99%

Bu-Shen-Zhu-Yun decoction induces PRLR deubiquitination and JAK2/STAT5 activation via CSN5 in vitro

Huang

Zhou

Lü

et al. 2021

Aging

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Purpose: To determine the effect of Bu-Shen-Zhu-Yun Decoction (BSZY-D) on the kisspeptin through JAK2/STAT5 signaling pathway in hyperprolactinemia (HPRL) infertility. Method: SD rats were treated with BSZY-D for cerebrospinal fluid (CSF) extraction. GT1-7 cells were subjected to different treatments. The phosphorylation levels of JAK2 and STAT5, and the expressions of PRLR and kisspeptin of GT1-7 cells in different groups were detected by western blot, RT-qPCR and immunofluorescence. The expressions of CSN5 and GATA1 and other molecular features were checked by western blot, RT-PCR, co-immunoprecipitation and renilla luciferase activity. Results: The phosphorylation levels of JAK2 and STAT5, and the expressions of PRLR and kisspeptin in the HPRL group were significantly decreased, and these changes could be reversed after BSZY-D treatment. In addition, the presence of PRLR deubiquitination was detected in the HPRL group, which could be reversed by shRNA-CSN5, suggesting that BSZY-D played a role through targeting CSN5. The binding level of GATA1 and CSN5 promoter in the HPRL group was significantly decreased, but elevated in the HPRL (BSZY-D/CSF) group (P < 0.05). Conclusion: BSZY-D improved the transcription activity of GATA1 and increased the binding of GATA1 and CSN5. BSZY-D was involved in the deubiquitination of PRLR, which contributes to alleviating the symptoms of HPRL infertility.

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“…For HPRL with non-neurological diseases and tumor compression, when serum PRL decreases, HPO function is gradually restored, enabling hypothalamic kisspeptin expression to normalize the hypothalamic-pituitary-gonadal (HPG) axis (18,19) Serum KP is a peptide hormone widely distributed in the brain and multiple tissues and regulates cancer cells, reproductive function, and endocrine function. Kisspeptin regulates brain regions associated with individual behavior, and kisspeptin is a possible target of psychosocial stress that inhibits LH (luteinizing hormone) impulses, while the regulation of serum KP can stimulate the secretion of LH and the FSH (20). BSZY-D can increase serum KP expression by affecting widespread prolactin receptors in hypothalamus GT1-7 cells.…”

Section: Discussionmentioning

confidence: 99%

The effects of combined bromocriptine and Bu-shen-zhu-yun decoction on serum hormones, anxiety, and pregnancy in hyperprolactinemic infertility patients

Huang¹,

Zhou²,

Jiang³

et al. 2021

Ann Palliat Med

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Background: Prolactin (PRL) is a protein hormone secreted by the anterior pituitary gland that regulates pituitary hormones. Hyperprolactinemia (HPRL), a pathological phenomenon of excessive PRL, can cause infertility in severe cases and is currently treated mainly with Western drugs, such as bromocriptine, a dopamine agonist (DA). Unfortunately, DAs produce psychological side effects which limit their long-term use. Traditional Chinese medicine (TCM) has minimal side effects and good results spanning many years of research. The combined treatment of TCM and Western medicine may enhance treatment efficacy and improve the long-term prognosis in HPRL. To analyze the effects of Bu-shen-zhu-yun decoction (BSZY-D) combined with bromocriptine on serum hormones, anxiety, and pregnancy in hyperprolactinemic infertile patients.Methods: One hundred patients diagnosed with HPRL infertility from June 2020 to June 2021 in the gynecology clinic of Jiangsu Provincial Hospital of Traditional Chinese Medicine were selected and grouped by envelope method. After excluding patients who withdrew or missed visits, 37 cases assigned to the control group were treated with bromocriptine, and 40 cases assigned to the observation group were treated with bromocriptine combined with BSZY-D. The patients' PRL and kisspeptin (KP) serum indexes, improvements in infertility, Anxiety Self-Assessment Scale (SAS) scores, and improvements in the Insomnia Severity Index Scale (ISI) scores were compared between the two groups.Results: At 3 and 6 months of treatment, serum PRL, SAS, and ISI scores were significantly lower, and serum KP was significantly higher in the observation group than in the control group (P<0.05). During the study period, the pregnancy rates were 62.50% (25/40) and 37.84% (14/37) in the observation and control groups, respectively. The observation group also had significantly fewer early miscarriages [10.00% (4/40) vs. 32.43% (12/37)] and less adverse reactions [7.50% (3/40) vs. 24.32% (9/37)] than the control group (all P<0.05). Conclusions:The combination of bromocriptine with BSZY-D was superior to bromocriptine alone in treating HPRL and HPRL-related infertility, which also demonstrated a positive effect on patients' sleep and low mood.

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Bu-shen-zhu-yun decoction promotes synthesis and secretion of FSHβ and LHβ in anterior pituitary cells in vitro

Cited by 8 publications

References 28 publications

Gegen Qinlian Decoction Ameliorates Hepatic Insulin Resistance by Silent Information Regulator1 (SIRT1)-Dependent Deacetylation of Forkhead Box O1 (FOXO1)

Gegen Qinlian Decoction Ameliorates Hepatic Insulin Resistance by Silent Information Regulator1 (SIRT1)-Dependent Deacetylation of Forkhead Box O1 (FOXO1)

Bu-Shen-Zhu-Yun decoction induces PRLR deubiquitination and JAK2/STAT5 activation via CSN5 in vitro

The effects of combined bromocriptine and Bu-shen-zhu-yun decoction on serum hormones, anxiety, and pregnancy in hyperprolactinemic infertility patients

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