Bub3 activation and inhibition of the APC/C

Yang, Yang; Lacefield, Soni

doi:10.1080/15384101.2015.1106746

Cited by 12 publications

(11 citation statements)

References 8 publications

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“…Then 29 records were excluded because they lacked necessary information, did not study on OSCC patients, and were duplicate reports on the same patients or reviews. Finally, 13 studies [11–17,22–27] with 1301 subjects were included for meta-analysis. The flowchart of study selection was illustrated in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…1. Nine studies [12–14,16,23–27] investigated the relationship between GLUT-1 and clinical parameters in OSCC and 8 studies [11–17,23] reported the association between GLUT-1 and OS. The included studies were published from 2003 to 2016 and the sample sizes ranged from 24 to 274.…”

Section: Resultsmentioning

confidence: 99%

“…The results pooled from 7 studies [13,16,23–27] showed that patients with advanced stages (III+IV) had high GLUT-1 expression than early stages (I+II) patients: OR = 2.99, 95% CI: 2.01–4.46, P < 0.001 (Fig. 2A).…”

Section: Resultsmentioning

confidence: 99%

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Prognostic value of GLUT-1 expression in oral squamous cell carcinoma

Sun

Gong

et al. 2016

Medicine

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Background:A variety of studies have evaluated the correlation between glucose transporter-1 (GLUT-1) expression and prognosis of oral squamous cell carcinoma (OSCC); however, the results were inconsistent and inconclusive. A meta-analysis was performed to assess the prognostic significance of GLUT-1 in OSCC.Methods:Electronic databases of PubMed, Embase, and Web of Science were searched for relevant studies. The last search was updated on July 2016. Odds ratio (OR) and 95% confidence interval (CI) were pooled to evaluate the relationship between GLUT-1 and clinical features and hazard ratio (HR) and 95% CI were combined to measure the effect of GLUT-1 on overall survival (OS). P value < 0.05 was considered as statistically significant.Results:A total of 13 studies with 1301 subjects were included for meta-analysis. The pooled data showed that high GLUT-1 expression was associated with advanced tumor stages (n = 7, OR = 2.99, 95% CI: 2.01–4.46, P < 0.001), higher tumor grade (n = 5, OR = 3.34, 95%CI: 1.12–9.94, P = 0.031), tumor size (n = 5, OR = 3.36, 95%CI: 2.04–5.51, P < 0.001), lymph node metastasis (n = 5, OR = 3.15, 95%CI: 1.89–5.25, P < 0.001), tobacco use (n = 3, OR = 2.18, 95%CI: 1.18–4.01, P = 0.013), and distant metastasis (n = 2, OR = 3.06, 95%CI: 1.19–7.9, P = 0.02). Furthermore, increased GLUT-1 expression was also correlated with shorter OS (n = 8, HR = 1.88, 95%CI: 1.51–2.33, P < 0.001). No significant publication bias was detected in this meta-analysis.Conclusion:GLUT-1 overexpression was in connection with aggressive clinical features and worse OS in OSCC. However, further studies are still needed to verify whether GLUT-1 could serve as a prognostic biomarker for OSCC.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Prognostic value of GLUT-1 expression in oral squamous cell carcinoma

Sun

Gong

et al. 2016

Medicine

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“…Tof1 and Csm3 are two proteins that ride with the replisome and appear to pause replication forks at protein-DNA barriers, but how they carry out this function is not understood (Calzada et al 2005; Mohanty et al 2006; Tourriere et al 2005). Mcm10 is an essential replication factor that is reported to interact with ssDNA, dsDNA, Pol α-primase, the CMG helicase, and other factors (reviewed in Bielinsky 2016; Thu and Bielinsky 2013). Mcm10 appears to be the last initiation protein to act at an origin, because two complete CMGs form at an origin yet cannot produce ssDNA until Mcm10 is present (Heller et al 2011; Kanke et al 2012; Watase et al 2012; Yeeles et al 2015).…”

Section: 9 Future Perspectivesmentioning

confidence: 99%

Architecture of the Saccharomyces cerevisiae Replisome

Bai

Yuan

Sun

et al. 2017

Advances in Experimental Medicine and Biology

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Eukaryotic replication proteins are highly conserved, and thus study of Saccharomyces cerevisiae replication can inform about this central process in higher eukaryotes including humans. The S. cerevisiae replisome is a large and dynamic assembly comprised of ~50 proteins. The core of the replisome is composed of 31 different proteins including the 11-subunit CMG helicase; RFC clamp loader pentamer; PCNA clamp; the heteroligomeric DNA polymerases ε, δ, and α-primase; and the RPA heterotrimeric single strand binding protein. Many additional protein factors either travel with or transiently associate with these replisome proteins at particular times during replication. In this chapter, we summarize several recent structural studies on the S. cerevisiae replisome and its subassemblies using single particle electron microscopy and X-ray crystallography. These recent structural studies have outlined the overall architecture of a core replisome subassembly and shed new light on the mechanism of eukaryotic replication.

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“…SAC is activated during the process of cell division, preventing separation of duplicated chromosomes until each chromosome is properly attached to the spindle apparatus. When SAC is not active, BUB3 facilitates binding of APC/C and Cdc20 for substrate ubiquitination [6]. Tumor-specific pyruvate kinase M2 (PKM2), a key enzyme in aerobic glycolysis, binds to Bub3 during mitosis and phosphorylates Bub3 at Y207.…”

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confidence: 99%

Inhibition of BUB3 shunts glucose to glycolytic pathway by inducing PFKFB3 accumulation

Zhang

Huang

et al. 2019

Preprint

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Purpose: Metabolic reprogramming as a hallmark of cancer has countless connections with other biological behavior of tumor such as rapid mitosis. Mitotic checkpoint protein BUB3 as a key protein involved in the regulation of mitosis is modulated by PKM2, an important glycolytic enzyme. However the role of BUB3 in glucose metabolism remains unknown. Methods: We analyzed the TCGA data to evaluate BUB3 expression in certain tumors. The uptake of glucose and CO2 incorporation was tested by isotopic tracer methods. The lactate, NADPH, NADP and metabolic enzyme activities were tested by assay kits accordingly. Results: We show here that BUB3 is over expressed in cervical cancer and hepatocellular carcinoma. Interference of BUB3 increase the uptake of glucose and shunts the metabolic flux from pentose phosphate pathway to glycolytic pathway. The glycolysis metabolites lactate is increased by BUB3 interference whereas NADPH/NADP ratio is reduced. With regard to metabolic enzymes, interference of BUB3 increase PFKFB3 on protein level and enzyme activity, but not mRNA level. Moreover, the increasing of protein level is diminished when proteasome degradation pathway is blocked by MG132. Conclusions: BUB3 is a potential tumor promoter and plays certain roles in cancer cellular metabolic reprogramming.

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Bub3 activation and inhibition of the APC/C

Cited by 12 publications

References 8 publications

Prognostic value of GLUT-1 expression in oral squamous cell carcinoma

Prognostic value of GLUT-1 expression in oral squamous cell carcinoma

Architecture of the Saccharomyces cerevisiae Replisome

Inhibition of BUB3 shunts glucose to glycolytic pathway by inducing PFKFB3 accumulation

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