Bubble biofilm: Bacterial colonization of air-air interface

Sjöberg, Susanne; Stairs, Courtney W.; Allard, Bert; Hallberg, Rolf; Homa, Félix; Martin, Tom; Ettema, Thijs J. G.; Dupraz, Christophe

doi:10.1016/j.bioflm.2020.100030

Cited by 7 publications

(8 citation statements)

References 13 publications

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“…4 ) (Sjöberg et al . 2020 ). This group of strictly cheomorganotrophic aerobes are mainly found in shallow water environments where they form floating biofilms at the air-water interface, defined as epineuston (Stürmeyer et al .…”

Section: Resultsmentioning

confidence: 99%

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Microbiomes in a manganese oxide producing ecosystem in the Ytterby mine, Sweden: impact on metal mobility

Sjöberg

Stairs

Allard

et al. 2020

FEMS Microbiology Ecology

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Microbe-mediated precipitation of Mn-oxides enriched in rare earth elements (REE) and other trace elements was discovered in tunnels leading to the main shaft of the Ytterby mine, Sweden. Defining the spatial distribution of microorganisms and elements in this ecosystem provide a better understanding of specific niches and parameters driving the emergence of these communities and associated mineral precipitates. Along with elemental analyses, high-throughput sequencing of the following four subsystems were conducted: (1) water seeping from a rock fracture into the tunnel, (2) Mn-oxides and associated biofilm; referred to as the Ytterby Black Substance (YBS) biofilm (3) biofilm forming bubbles on the Mn-oxides; referred to as the bubble biofilm, and (4) fracture water that has passed through the biofilms. Each subsystem hosts a specific collection of microorganisms. Differentially abundant bacteria in the YBS biofilm were identified within the Rhizobiales (e.g. Pedomicrobium), PLTA13 Gammaproteobacteria, Pirellulaceae, Hyphomonadaceae, Blastocatellia and Nitrospira. These taxa, likely driving the Mn-oxide production, were not detected in the fracture water. This biofilm binds Mn, REE and other trace elements in an efficient, dynamic process, as indicated by substantial depletion of these metals from the fracture water as it passes through the Mn deposit zone. Microbe-mediated oxidation of Mn(II) and formation of Mn(III/IV)-oxides can thus have considerable local environmental impact by removing metals from aquatic environments.

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Section: Resultsmentioning

confidence: 99%

“…The seeping water provides elements and nutrients for two distinctively different biofilms to form: a Mn-oxide producing biofilm (hereafter referred to as the YBS biofilm) and an associated gas-trapping biofilm (hereafter referred to as the bubble biofilm and the subject of a separate paper; Sjöberg et al . 2020 ) (Fig. 1C and D ).…”

Section: Introductionmentioning

confidence: 91%

Microbiomes in a manganese oxide producing ecosystem in the Ytterby mine, Sweden: impact on metal mobility

Sjöberg

Stairs

Allard

et al. 2020

FEMS Microbiology Ecology

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“…Members of the Pedobacter and Nevskia genera were previously not known to oxidize Mn while the three other taxa were known to. Members of the Nevskia genus were previously shown to be highly abundant at the field site, while the relative abundance of the other strains only accounted for a maximum of 0.09% [8,32]. Hydrogenophaga spp.…”

Section: Isolated Mn-oxidizing Microorganismsmentioning

confidence: 91%

“…cells. Nevskia spp., which are epineuston stalked bacteria (bacteria living at the water surface and producing thin extensions out of the cell wall), representing the most abundant genus in a micrometer thick gas-trapping biofilm located on the surface of the precipitated Mn oxides in Ytterby (see details in [8,32]). Its involvement in the Mn oxide producing ecosystem is not yet clear, but results from this study indicate that Nevskia spp.…”

Section: Pedobacter Spmentioning

confidence: 99%

“…The promotion of Mn oxidation by biofilm formation was previously documented by Nealson and Ford (1980) [65], who observed that a Bacillus species had little or no Mn-oxidizing capability as a free floating cell, but showed a drastic increase in Mn oxidation rate once the bacterium interacted with solid substrates. Deposition of Mn oxides in the Ytterby mine tunnel is also associated with a group of bacteria forming epilithic biofilms [8,32]. This type of biofilm is beneficial for Mn oxidation partly due to the potential as nucleation site for Mn mineralization, but also for the capacity of binding elements to acidic groups within the biofilm, thus acting as storage for further use or for neutralization purposes.…”

Section: Promotion Of Mn Oxidation By Biofilm Formationmentioning

confidence: 99%

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Microbe-Mediated Mn Oxidation—A Proposed Model of Mineral Formation

Sjöberg

Stairs

et al. 2021

Minerals

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Manganese oxides occur in a wide range of environmental settings either as coatings on rocks, sediment, and soil particles, or as discrete grains. Although the production of biologically mediated Mn oxides is well established, relatively little is known about microbial-specific strategies for utilizing Mn in the environment and how these affect the morphology, structure, and chemistry of associated mineralizations. Defining such strategies and characterizing the associated mineral properties would contribute to a better understanding of their impact on the local environment and possibly facilitate evaluation of biogenicity in recent and past Mn accumulations. Here, we supplement field data from a Mn rock wall deposit in the Ytterby mine, Sweden, with data retrieved from culturing Mn oxidizers isolated from this site. Microscopic and spectroscopic techniques are used to characterize field site products and Mn precipitates generated by four isolated bacteria (Hydrogenophaga sp., Pedobacter sp., Rhizobium sp., and Nevskia sp.) and one fungal-bacterial co-culture (Cladosporium sp.—Hydrogenophaga sp. Rhizobium sp.—Nevskia sp.). Two of the isolates (Pedobacter sp. and Nevskia sp.) are previously unknown Mn oxidizers. At the field site, the onset of Mn oxide mineralization typically occurs in areas associated with globular wad-like particles and microbial traces. The particles serve as building blocks in the majority of the microstructures, either forming the base for further growth into laminated dendrites-botryoids or added as components to an existing structure. The most common nanoscale structures are networks of Mn oxide sheets structurally related to birnessite. The sheets are typically constructed of very few layers and elongated along the octahedral chains. In places, the sheets bend and curl under to give a scroll-like appearance. Culturing experiments show that growth conditions (biofilm or planktonic) affect the ability to oxidize Mn and that taxonomic affiliation influences crystallite size, structure, and average oxidation state as well as the onset location of Mn precipitation.

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