Bucentaur (Bcnt) Gene Family: Gene Duplication and Retrotransposon Insertion

Iwashita, Shintaro; Osada, Naoki

doi:10.5772/22351

Cited by 7 publications

(18 citation statements)

References 45 publications

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“…Df(2Rh), Df(2R)MS41A1, a deletion lacking the Yeti gene region (see Materials and Methods). The migration of YETI protein (apparent molecular mass of 32 kDa) is somewhat slower than predicted (about 27 kDa), as also reported for bovine BCNT proteins, possibly owing to the physical properties of these intrinsically disordered proteins lacking a stable three-dimensional structure (Iwashita and Naoki, 2011). morphology was seen ( Fig. 3C) and the staining pattern was similar to that found in elav-GAL4; Yeti-GFP flies (Fig.…”

Section: Exogenously Expressed Yeti Protein Rescues the Chromosome Mosupporting

confidence: 53%

“…The Drosophila melanogaster BCNT protein, YETI, was originally defined as a kinesin-binding protein able to bind both subunits of the microtubule-based motor kinesin-I (Wisniewski et al, 2003). Related BCNT proteins have been found in both cytoplasmic and nuclear fractions (Iwashita and Naoki, 2011), and they have been shown to asociate with kinetochores in chicken (Ohta et al, 2010). Additional studies have linked the BCNT proteins to craniofacial development and osteogenesis, though limited mechanistic understanding is available.…”

Section: Introductionmentioning

confidence: 99%

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Yeti, aDrosophila melanogasteressential gene, encodes a protein required for chromatin organization

Messina

Damia

Fanti

et al. 2014

Journal of Cell Science

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The evolutionarily conserved family of Bucentaur (BCNT) proteins exhibits a widespread distribution in animal and plants, yet its biological role remains largely unknown. Using Drosophila melanogaster as a model organism, we investigated the in vivo role of the Drosophila BCNT member called YETI. We report that loss of YETI causes lethality before pupation and defects in higherorder chromatin organization, as evidenced by severe impairment in the association of histone H2A.V, nucleosomal histones and epigenetic marks with polytene chromosomes. We also find that YETI binds to polytene chromosomes through its conserved BCNT domain and interacts with the histone variant H2A.V, HP1a and Domino-A (DOM-A), the ATPase subunit of the DOM/Tip60 chromatin remodeling complex. Furthermore, we identify YETI as a downstream target of the Drosophila DOM-A. On the basis of these results, we propose that YETI interacts with H2A.Vexchanging machinery, as a chaperone or as a new subunit of the DOM/Tip60 remodeling complex, and acts to regulate the accumulation of H2A.V at chromatin sites. Overall, our findings suggest an unanticipated role of YETI protein in chromatin organization and provide, for the first time, mechanistic clues on how BCNT proteins control development in multicellular organisms.

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Section: Exogenously Expressed Yeti Protein Rescues the Chromosome Mosupporting

confidence: 53%

Section: Introductionmentioning

confidence: 99%

Yeti, aDrosophila melanogasteressential gene, encodes a protein required for chromatin organization

Messina

Damia

Fanti

et al. 2014

Journal of Cell Science

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“…Cfdp1 is 139,815 bp long with 7 exons and 6 introns and maps to chromosome 16 in the band 16q22.2-q22.3, where it is proximally and distally flanked by Bcar1 and Tmem170A genes, respectively12131415. Cfdp1 expression has been detected in a wide range of human tissues, including cancer tissues.…”

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confidence: 99%

“…Cfdp1 expression has been detected in a wide range of human tissues, including cancer tissues. It encodes a protein of 299 amino acids, called CFDP1, belonging to the evolutionarily conserved family of Bucentaur (BCNT) proteins12131415. The CFDP1 protein is characterized by an 82-amino acid region located at the C-terminus, called the BCNT-C domain, which is highly conserved among almost all eukaryotes, while the N-terminal region is more divergent121314.…”

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confidence: 99%

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The human Cranio Facial Development Protein 1 (Cfdp1) gene encodes a protein required for the maintenance of higher-order chromatin organization

Messina

Atterrato

Prozzillo

et al. 2017

Sci Rep

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The human Cranio Facial Development Protein 1 (Cfdp1) gene maps to chromosome 16q22.2-q22.3 and encodes the CFDP1 protein, which belongs to the evolutionarily conserved Bucentaur (BCNT) family. Craniofacial malformations are developmental disorders of particular biomedical and clinical interest, because they represent the main cause of infant mortality and disability in humans, thus it is important to understand the cellular functions and mechanism of action of the CFDP1 protein. We have carried out a multi-disciplinary study, combining cell biology, reverse genetics and biochemistry, to provide the first in vivo characterization of CFDP1 protein functions in human cells. We show that CFDP1 binds to chromatin and interacts with subunits of the SRCAP chromatin remodeling complex. An RNAi-mediated depletion of CFDP1 in HeLa cells affects chromosome organization, SMC2 condensin recruitment and cell cycle progression. Our findings provide new insight into the chromatin functions and mechanisms of the CFDP1 protein and contribute to our understanding of the link between epigenetic regulation and the onset of human complex developmental disorders.

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The Bucentaur (BCNT) protein family: a long-neglected class of essential proteins required for chromatin/chromosome organization and function

et al. 2014

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The evolutionarily conserved Bucentaur (BCNT) protein superfamily was identified about two decades ago in bovines, but its biological role has long remained largely unknown. Sparse studies in the literature suggest that BCNT proteins perform important functions during development. Only recently, a functional analysis of the Drosophila BCNT ortholog, called YETI, has provided evidence that it is essential for proper fly development and plays roles in chromatin organization. Here, we introduce the BCNT proteins and comprehensively review data that contribute to clarify their function and mechanistic clues on how they may control development in multicellular organisms.

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Bucentaur (Bcnt) Gene Family: Gene Duplication and Retrotransposon Insertion

Cited by 7 publications

References 45 publications

Yeti, aDrosophila melanogasteressential gene, encodes a protein required for chromatin organization

Yeti, aDrosophila melanogasteressential gene, encodes a protein required for chromatin organization

The human Cranio Facial Development Protein 1 (Cfdp1) gene encodes a protein required for the maintenance of higher-order chromatin organization

The Bucentaur (BCNT) protein family: a long-neglected class of essential proteins required for chromatin/chromosome organization and function

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