Buckwheat (Fagopyrum esculentum M.) Sprout Treated with Methyl Jasmonate (MeJA) Improved Anti-Adipogenic Activity Associated with the Oxidative Stress System in 3T3-L1 Adipocytes

Abstract

Buckwheat sprouts contain various bioactive compounds including rutin which have a number of biological activities. We have previously shown that buckwheat sprouts (TBWE) treated with methyl jasmonate (MeJA) significantly increased the amount of phenolics and the antioxidant activity. The aim of this study was to demonstrate the effect of TBWE on anti-adipogenesis and pro-oxidant enzyme in 3T3-L1 adipocytes. We also evaluated the anti-oxidative activity of TBWE in adipocytes by using the nitroblue tetrazolium assay. Our data showed that TBWE markedly inhibited adipocyte differentiation and ROS production in 3T3-L1 cells compared with control groups. Moreover, TBWE has strongly shown the inhibition of adipogenic transcription factor as well as pro-oxidant enzymes. Together, we demonstrate that the MeJA treatment significantly increased the amount of phenolic compound, resulting in the suppression of adipogenesis and ROS production in the 3T3-L1 cells. These findings indicate that TBWE has the potential for anti-adipogenesis activity with anti-oxidative properties.

Redox modulation of adipocyte differentiation: hypothesis of “Redox Chain” and novel insights into intervention of adipogenesis and obesity

“…Polygonum aviculare L. ethanol extract exhibits anti-diabetic effect in HF diet-induced obese mice [333]. Buckwheat sprouts exhibits anti-adipogenic and anti-oxidative properties [334].…”

Optimization of methyl jasmonate application to broccoli florets to enhance health-promoting phytochemical content

“…MeJA application can enhance total phenolic concentrations in radish, buckwheat sprouts and sweet basil . Several studies have shown that MeJA‐mediated increases in flavonoids or phenolics can enhance antioxidant, antiproliferative and antiadipogenic activities of sweet basil, buckwheat and blackberry . Pérez‐Balibrea et al reported that exogenous application of MeJA increased glucosinolate (GS) concentrations in broccoli sprouts.…”

“…and lepidopteran species (Pieris rapae and Trichoplusia ni). MeJA application can enhance total phenolic concentrations in radish, 8 buckwheat sprouts 9 and sweet basil. 10 Several studies have shown that MeJA-mediated increases in flavonoids or phenolics can enhance antioxidant, antiproliferative and antiadipogenic activities of sweet basil, buckwheat and blackberry.…”

“…10 Several studies have shown that MeJA-mediated increases in flavonoids or phenolics can enhance antioxidant, antiproliferative and antiadipogenic activities of sweet basil, buckwheat and blackberry. 9 -11 Pérez-Balibrea et al 12 reported that exogenous application of MeJA increased glucosinolate (GS) concentrations in broccoli sprouts. For these reasons, JAs could be used in vegetable production fields to enhance consumer health promotion and provide value-added marketing opportunities.…”

A review on Fagopyrum esculentum: A potential medicinal plant

“…Moreover, TBWE was strongly inhibited adipogenic transcription factor as well as pro-oxidant enzymes. Methyl jasmonate treatment significantly increased the amount of phenolic compound, resulting in the suppression of adipogenesis and ROS production in the 3T3-L1 cells [74]. The acute effect of consumption of 500 ml of water, water with buckwheat honey, black tea, black tea with sugar, or black tea with buckwheat honey, on serum oxidative reactions was examined in 25 healthy men.…”