Buckwheat self-assembling peptide-based hydrogel: Preparation, characteristics and forming mechanism

Yu, Mengru; Ge, Rui; Zhang, Junping; Xiong, Chunyu; Xu, Linlin; Zhao, Mengting; Fan, Jie

doi:10.1016/j.foodhyd.2021.107378

Cited by 30 publications

(32 citation statements)

References 28 publications

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“…Future studies on determination of thiol (SH) and disulfide bond (S–S) could be helpful to understand the role of covalent interactions in the gel formation of QPI hydrolysates. Determining peptide sequences using LC-MS/MS and evaluating their aggregation and gelation tendencies using computational tools ( Yu et al, 2022 ) will further elucidate the gelation mechanism. This study also provides a new route to fabricate thermally induced QPI gels via enzymatic hydrolysis for potential industrial production of food products such as sausages, meat substitutes, and tofu-like products.…”

Section: Discussionmentioning

confidence: 99%

Limited Alcalase hydrolysis improves the thermally-induced gelation of quinoa protein isolate (QPI) dispersions

Wang

Cheng

Wang

et al. 2022

Current Research in Food Science

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Section: Discussionmentioning

confidence: 99%

Limited Alcalase hydrolysis improves the thermally-induced gelation of quinoa protein isolate (QPI) dispersions

Wang

Cheng

Wang

et al. 2022

Current Research in Food Science

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“…HSSC was thawed at room temperature. The amino acids of HSSC were determined according to Yu et al 18 HSSC was dispersed in Milli-Q water (1:100, w/v) and hydrolyzed in a shaking bath with alkaline protease (pH 9.0, 50 °C), neutral protease (pH 7.0, 37 °C), bromelain (pH 7.5, 50 °C), papain (pH 6.0, 37 °C), and trypsin (pH 7.0, 37 °C). Enzymes were added to the solution at a ratio of 300 U/g (enzyme/protein) to initiate.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Novel Peptide from the Hydrolysate of Hybrid Sturgeon (Acipenseridae) Spinal Cord: Isolation, Identification, and Anti-proliferative Effects in Human Cervix Cancer Cells

Han

Wang²,

et al. 2022

J. Agric. Food Chem.

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Anti-proliferative peptides have recently attracted attention for their excellent bioactivity and biocompatibility. In this paper, five novel anti-proliferative peptides were identified from the hydrolysate of hybrid sturgeon spinal cord (HSSC). In addition, the structure−activity relationship of the novel anti-proliferative peptides was explored. In vitro experiments indicated that the peptide "VDSVLDVVRK" presented the highest inhibition of HeLa cell growth in all samples (IC 50 = 2.5 μM). VDSVLDVVRK showed a random coil secondary structure and nanomicelles in the tumor microenvironment. Transmission electron microscopy results confirmed that nanomicelles disassemble as the concentration of VDSVLDVVRK decreases. Furthermore, VDSVLDVVRK could induce HeLa cell apoptosis by increasing the expression of Cyt-c (98.65 ± 1.85%, p < 0.01) and caspase-9 (39.85 ± 1.81%, p < 0.01). In this study, the anti-proliferative mechanism of the HSSC peptide was discussed, which provided a theoretical basis for the research and development of anti-proliferative functional food.

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“…In the process of movement, peptides assemble in an irregular manner. Following that, hydrophobic and other interactions drive peptides to permute orderly and eventually shape a hydrogel (Criado-Gonzalez et al, 2020;Ruter et al, 2020;Yu et al, 2022). For example, MAX1 peptide, which in aqueous solution offers a hydrophobic interface of valine (V) residues and a hydrophilic interface of lysine (K) residues, conducted lateral assembly through hydrophobic interaction among side chains, finally generating a noncovalent crosslinking network (Lin et al, 2011).…”

Section: Hydrophobic Interactionmentioning

confidence: 99%

“…Although tetrapeptides are the most common structure, dipeptides and tripeptides can form β-turn as well (Boussard and Marraud, 1985). Yu et al described how the secondary structure of peptides realized the transformation from βsheet to α-helix and β-turn in the process of SAPH formation, and βturn was pivotal to stabilizing the SAPH (Yu et al, 2022).…”

Section: Formation Of Self-assembling Peptide Hydrogelsmentioning

confidence: 99%

“…In addition, Guilbaud et al found that FEFK tetrapeptide was hydrolyzed by a protease enzyme to develop two kinds of octapeptides, FEFEFKFK and FEFKFEFK, which were prone to self-assembly, and the presence of the enzyme led to the Frontiers in Materials frontiersin.org improvement of mechanical properties of SAPHs as well (Guilbaud et al, 2013). Yu et al found that after 120 or 150 min of hydrolysis by alkaline protease, buckwheat proteins could self-assemble into a SAPH without any chemical induction or treatment (Yu et al, 2022).…”

Section: Enzymementioning

confidence: 99%

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Research progress of self-assembling peptide hydrogels in repairing cartilage defects

Wang

Yang

et al. 2022

Front. Mater.

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Due to the lack of blood vessels, nerves and lymphatic vessels, the capacity of articular cartilage to heal is extremely limited. Once damaged, it is urgent for articular cartilage to repair the injury. In recent years, there has been an increase in cartilage tissue engineering studies. Self-assembling peptide hydrogel as a kind of hydrogels composed of peptides and water is widely used in cartilage tissue engineering. Under noncovalent interactions such as electrostatic interaction, hydrophobic interaction, hydrogen bonding and pi-pi stacking force, peptides self-assemble into three-dimensional (3D) structures that mimic the natural extracellular matrix and allow cells to grow, proliferate and differentiate. Because SAPHs have excellent biocompatibility and biodegradability, variable mechanical properties, low immunogenicity, injectability, and the ability to load cells and bioactive substances, many researchers utilized them to promote the repair and regeneration of articular cartilage after damage. Therefore, the purpose of this review is to sum up the composition, injury characteristics, and treatments of articular cartilage, as well as the action of SAPHs in repairing articular cartilage damage.

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Buckwheat self-assembling peptide-based hydrogel: Preparation, characteristics and forming mechanism

Cited by 30 publications

References 28 publications

Limited Alcalase hydrolysis improves the thermally-induced gelation of quinoa protein isolate (QPI) dispersions

Limited Alcalase hydrolysis improves the thermally-induced gelation of quinoa protein isolate (QPI) dispersions

Novel Peptide from the Hydrolysate of Hybrid Sturgeon (Acipenseridae) Spinal Cord: Isolation, Identification, and Anti-proliferative Effects in Human Cervix Cancer Cells

Research progress of self-assembling peptide hydrogels in repairing cartilage defects

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