Buffalo (<i>Bubalus bubali</i>) Late Embryo and Foetus Development: A Morphological Analysis

Morini, Adriana Caroprezo; Pieri, Naira Caroline Godoy; Roballo, Kelly Cristine Santos; Martins, D. S.; Ambrósio, Carlos Eduardo; Morini, J. C.; Favaron, Phelipe Oliveira; Minervino, Antônio Humberto Hamad; Pereira, F. V. T.; Miglino, Maurizio

doi:10.1111/rda.12712

Cited by 4 publications

(3 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present study the digestive tube was first observed on the 23rd day as in sheep, buffalo and equine feti at 25th day of gestation (Morini et al, 2016;Rodrigues et al, 2014;Trahair & Robinson, 1986), respectively. The primitive intestine was covered by mesenchymal mass on the 23rd day similarly to equine feti at 25th day of gestation (Franciollia et al, 2011).…”

Section: Histogenesis Of Intestinementioning

confidence: 52%

Embryonic differentiation of intestine in Indian goats (Capra hircus)

Verma

Farooqui

Prakash

et al. 2023

Anat Histol Embryol

View full text Add to dashboard Cite

The present study focused on identifying knowledge gaps in scientific literature for developmental anatomy and future fetal‐programming researches on goats. The aim of this study was to observe the sequential growth and the development of the intestine from stage of formation of preliminary digestive tube to the differentiation of various segments during early prenatal life in Indian goats. The developing digestive tube was first witnessed on the 23rd day of gestation histologically and from 25 days of gestation onwards, it became observable grossly under stereozoom microscope. The further stages of developing intestine rotation, herniation and coiling and re‐entry stages were noticed on the 38th day, 41st day and 48th day of gestation, respectively. The first demarcation between small and large intestines in the form of cecal bulge was recorded at 41st day of gestation. On the 45th day of gestation, all the segments of intestine that is, duodenum, jejunum, ileum, caecum, colon and rectum were well demarcated. Microscopically, the wall of the digestive tube was composed of three layers; lamina epithelialis, pleuripotent blastemic tissue and the mesothelium in between 23 and 39 days of gestation. At 41st day of gestation, the blastemic tissue showed distinct separation into lamina propria‐submucosa and tunica muscularis. Among connective tissue fibres only reticular fibres were observable in this study. The histochemical localisation of polysaccharides, bound lipids and alkaline phosphatase enzyme showed mild to moderate reaction. The reaction for acid phosphatase enzyme could not be observed or was absent in this study.

show abstract

Section: Histogenesis Of Intestinementioning

confidence: 52%

Embryonic differentiation of intestine in Indian goats (Capra hircus)

Verma

Farooqui

Prakash

et al. 2023

Anat Histol Embryol

View full text Add to dashboard Cite

show abstract

“…In total, 33 chorioallantoic membranes were collected in slaughterhouse at Macapá, (North of Brazil) from pregnant water buffalos with estimated gestational ages ranging from 30 to 53 days. Estimations of the gestational age were done on the basis of the crown‐rump length following standardized methods (Abdel‐Raouf & El‐Naggar, ) and according to the external features of the embryos and foetuses as previously published by our group (Morini et al, , ), see Table .…”

Section: Methodsmentioning

confidence: 99%

Characterization of the extracellular matrix in the chorioallantoic membrane of water buffalo (Bubalus bubalis) in early gestation

Borghesi

Ferreira

Favaron

et al. 2019

Reprod Domestic Animals

View full text Add to dashboard Cite

Placenta is formed by a parenchyma rich in extracellular matrix (ECM), and this structure guarantees the proper development of the embryo and placental functioning. Recently, studies have focused on the characterization of ECM in the placenta and foetal membranes of different species. This work aimed to analyse the composition of the ECM and to quantify the types of collagens in its composition. For this, 33 chorioallantoic membranes were used at different gestational ages, which were grouped into five groups. Subsequently, haematoxylin–eosin staining, Masson trichrome and picrosirius were performed for histological analysis. Through the technique of polarized light, it was possible to quantify the total collagen present in the membranes and finally the immunohistochemical technique was performed to verify the presence of collagens and glycoproteins. It was possible to verify that the chorioallantoic membranes have, in all the gestational periods of the initial third of gestation, the same histological structures, being the most significant difference the membrane thickening that occurs gradually during the gestation. However, we notice the appearance of binucleate cells only from group II. In addition, it was verified that a gradual increase of collagen occurs until the group IV, yet from the group V begins to occur a decrease of this protein. In addition, collagen I, collagen III, fibronectin and laminin were present in all membranes. With this, we concluded that the buffalo chorioallantoic membrane presents ECM in constant remodelling at the beginning of gestation and can be used as biomaterial in works on regenerative biology.

show abstract

“…For placental tissues, the samples were paired samples from the maternal and fetal parts of the uterine placentome of the same animal. Size of the crown rump length (CRL) of the fetus was used as a reference to calculate the stage of pregnancy (Figure 1; Edward, 1965;Soliman, 1975;Morini et al, 2016). Based on CRL, tissues from the animals of Day 25 and Days 30-35 of pregnancy were collected in cold Dulbecco's phosphate-buffered saline (DPBS) and washed and stored in RNA later (Fajardy et al, 2009;Aliniyaa et al, 2017).…”

Section: Tissue Sample Collectionmentioning

confidence: 99%

Establishment of Repertoire of Placentome-Associated MicroRNAs and Their Appearance in Blood Plasma Could Identify Early Establishment of Pregnancy in Buffalo (Bubalus bubalis)

Sarwalia

Raza

Soni

et al. 2021

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Precise early pregnancy diagnosis in dairy animals is of utmost importance for an efficient dairy production system. Not detecting a dairy animal pregnant sufficiently early after the breeding results to extending the unproductive time of their milk production cycle and causes substantial economic loss for a dairy producer. At present, the most conventional and authentic pregnancy confirmation practice in cows and buffaloes is rectal palpation of the reproductive organs at Days 35–40 after insemination, which sometime leads to considering an animal as false pregnant. Other alternative methods available for early pregnancy diagnosis lack either accuracy or reproducibility or require elaborate instrumentation and laboratory setup not feasible to practice at farmers’ doorstep. The present study was aimed at establishment of the microRNA (miRNA) repertoire of the placentome in buffaloes, which could capture the event of the cross talk between a growing embryo and a dam, through fetal cotyledons and maternal caruncles, and thus could hint at the early pregnancy establishment event in ruminants. Total RNA was isolated from buffalo placentome tissues during early stages of pregnancy (at Day < 25 and Days 30–35), and global small RNA analysis was performed by using Illumina single-end read chemistry and Bubalus bubalis genome. A total of 2,199 miRNAs comprising 1,620 conserved and 579 non-conserved miRNAs were identified. Stringent functional miRNA selection criteria could predict 20 miRNAs worth evaluating for their abundance in the plasma of pregnant, non-pregnant, cyclic non-bred, and non-cyclic prepubertal animals. Eight of them (viz., miR-195-5p, miR-708-3p, miR-379-5p, miR-XX1, miR-XX2, miR-130a-3p, miR-200a-3p, and miR-27) displayed typical abundance patterns in the plasma samples of the animals on Day 19 as well as Day 25 post-insemination, thus making them ambiguous candidates for early pregnancy detection. Similarly, higher abundance of miR-200a-3p and miR130a-3p in non-pregnant animals was indicative of their utility for detecting the animals as not pregnant. Most interestingly, miR-XX1 and miR-XX2 were very characteristically abundant only in pregnant animals. In silico target prediction analysis confirmed that these two miRNAs are important regulators of cyclooxygenase-2 (COX-2) and cell adhesion molecule-2 (CADM-2), both of which play a significant role in the implantation process during feto-maternal cross talk. We interpret that circulatory miR-XX1 and miR-XX2 in blood plasma could be the potential biomarkers for early pregnancy detection in buffaloes.

show abstract

Buffalo (Bubalus bubali) Late Embryo and Foetus Development: A Morphological Analysis

Cited by 4 publications

References 12 publications

Embryonic differentiation of intestine in Indian goats (Capra hircus)

Embryonic differentiation of intestine in Indian goats (Capra hircus)

Characterization of the extracellular matrix in the chorioallantoic membrane of water buffalo (Bubalus bubalis) in early gestation

Establishment of Repertoire of Placentome-Associated MicroRNAs and Their Appearance in Blood Plasma Could Identify Early Establishment of Pregnancy in Buffalo (Bubalus bubalis)

Contact Info

Product

Resources

About