Buffalo rumen harbours diverse thermotolerant yeasts capable of producing second-generation bioethanol from lignocellulosic biomass

Avchar, Rameshwar; Lanjekar, Vikram; Kshirsagar, Pranav R.; Dhakephalkar, Prashant K.; Dagar, Sumit Singh; Baghela, Abhishek

doi:10.1016/j.renene.2021.04.002

Cited by 11 publications

(26 citation statements)

References 49 publications

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“…The ethanol productivity of K. marxianus MSS6.3 and P. kudriavzevii DSA3.2 was higher in case of SSF (0.23 and 0.22 g l −1 h −1 ) compared to SHF (0.21 and 0.21 g l −1 h −1 ), respectively. These two yeasts (K. marxianus MSS6.3 and P. kudriavzevii DSA3.2) were comparable to the highest ethanol-producing yeast strains K. marxianus IMB3 and P. kudriavzevii RGB3.2 (Table S5) (Avchar et al, 2021;Pessani et al, 2011). However, our strains K. marxianus MSS6.3 and P. kudriavzevii DSA3.2…”

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confidence: 50%

“…The pH of withdrawn fermentation samples was set to 2.0 with 3 mol l −1 HCl, and then centrifuged for 15 min at 8000 g (4℃). The amount of ethanol produced was determined by HPLC-UPLC system following formerly described conditions (Avchar et al, 2021). All experiments were carried out in triplicates.…”

Section: Evaluation Of the Ethanol-producing Potential Of Yeast Strainsmentioning

confidence: 99%

“…43, 45 and 50℃). Samples were withdrawn after 48 h and the amount of sugar consumed and ethanol produced were measured through HPLC-UPLC system (Avchar et al, 2021). Saccharified slurry without yeast inoculum was considered as a control experiment and used for determining total released sugars post-saccharification.…”

Section: Ethanol Production Via Simultaneous Saccharification and Fer...mentioning

confidence: 99%

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Bioprospecting thermotolerant yeasts from distillery effluent and molasses for high‐temperature ethanol production

Avchar

Lanjekar

Baghela

2021

J of Applied Microbiology

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Aims: Isolation, characterization and assessment of inhibitor tolerance of thermotolerant yeasts associated with distillery effluent and molasses, and their use in hightemperature ethanol production from alkali-treated rice straw. Methods and Results:A total of 92 thermotolerant yeasts were isolated from seven different distillery effluent and molasses samples. Based on MSP-PCR, 34 yeasts were selected and identified by sequencing the D1/D2 domain of LSU rDNA. These yeasts belonged to eight genera and nine different species. We assessed the inhibitor tolerance of these 34 well-characterized yeasts against various pre-treatment-generated inhibitors (furfural, 5-hydroxymethyl furfural and acetic acid) and also evaluated their ethanol yields at 40, 45 and 50℃. Among selected strains, Pichia kudriavzevii DSA3.2 exhibited the highest ethanol production (24.5 g l −1 ) with an efficiency of 95.7% at 40℃ using 5% glucose. At 45℃, P. kudriavzevii DSA3.2 and Kluyveromyces marxianus MSS6.3 yielded maximum ethanol titres; 22.3 and 23 g l −1 with 87.4% and 90% efficiency, respectively. While using alkali-treated RS at 45℃, K. marxianus MSS6.3 produced 10.5 g l −1 of ethanol with 84.5% fermentation efficiency via separate hydrolysis and fermentation, and 10.9 g l −1 of ethanol with 85% efficiency via simultaneous saccharification and fermentation. Pichia kudriavzevii DSA3.2, DSA3.1 and K. marxianus MSS6.3 also exhibited significant tolerance against multiple inhibitors. Conclusions: Yeast isolates P. kudriavzevii DSA3.2 and K. marxianus MSS6.3 exhibited significant inhibitor tolerance and proved to be suitable for high-temperature ethanol fermentation. After additional optimization and scale-up experiments, these isolates can be exemplary candidates for industrial-scale ethanol production from lignocellulosic biomass. Significance and Impact of the Study:Our study recognizes distillery effluents and molasses as specialized niches for yeasts with a broad substrate range, capable of tolerating multiple inhibitors and yielding high levels of ethanol at elevated temperatures. These yeasts can further be exploited for bioethanol production through SSF/SHF at a larger scale.

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confidence: 50%

Section: Evaluation Of the Ethanol-producing Potential Of Yeast Strainsmentioning

confidence: 99%

Section: Ethanol Production Via Simultaneous Saccharification and Fer...mentioning

confidence: 99%

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Bioprospecting thermotolerant yeasts from distillery effluent and molasses for high‐temperature ethanol production

Avchar

Lanjekar

Baghela

2021

J of Applied Microbiology

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“…Furthermore, our previous study also found that IF can significantly enhance the production of extracellular MPs (exMPs) by M. purpureus M183 6 . Currently, similar to the production of second‐generation bio‐ethanol and bio‐butanol using fermentation technology by saccharomycetes 8 or clostridium , 9 the lost‐cost or waste products, especially the abundant agricultural materials (lignocellulosic materials), have been utilized to produce MPs by Monascus spp., to cut costs and encourage the sustainable utilization of raw materials, such as sugarcane bagasse, 10 corn cobs, 11 corn bran, 12 whey, 13 wheat, 14,15 waste orange peels, 16 spent grain, 17 loquat kernels, 18 and rice straw 19 …”

Section: Introductionmentioning

confidence: 99%

“…7 Furthermore, our previous study also found that IF can significantly enhance the production of extracellular MPs (exMPs) by M. purpureus M183. 6 Currently, similar to the production of second-generation bio-ethanol and bio-butanol using fermentation technology by saccharomycetes 8 or clostridium, 9 the lost-cost or waste products, especially the abundant agricultural materials (lignocellulosic materials), have been utilized to produce MPs by Monascus spp., to cut costs and encourage the sustainable utilization of raw materials, such as sugarcane bagasse, 10 corn cobs, 11 corn bran, 12 whey, 13 wheat, 14,15 waste orange peels, 16 spent grain, 17 loquat kernels, 18 and rice straw. 19 Rice husk is one of the main by-products of rice processing, and it has several characteristics, such as high cellulose (approximately 30-45%) and hemicellulose (approximately 19-34%) content, which can be converted into a carbon source (glucose, xylose, and arabinose) for MP production by Monascus strains in submerged fermentation.…”

Section: Introductionmentioning

confidence: 99%

Utilization of low‐cost agricultural by‐product rice husk for Monascus pigments production via submerged batch‐fermentation

et al. 2021

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BACKGROUND: Monascus pigments (MPs) produced by the genus Monascus, have been utilized for more than 2000 years in the food industry. In the present study, by submerged batch-fermentation (SBF), we were able to obtain a mutant strain with a high tolerance of inhibitory compounds generated from rice husk hydrolysate, allowing the production of MPs.RESULTS: The mutant strain, M. Purpureus M523 with high rice husk hydrolysate tolerance was obtained using the atmospheric and room temperature plasma (ARTP) screening system, producing 39.48 U mL −1 extracellular total MPs (yellow and orange MPs), using non-detoxified rice husk diluted sulfuric acid hydrolysate (RHSAH) as the carbon source in SBF. Extracellular MPs (exMPs) production was enhanced to 72.1 and 80.7 U mL −1 in supplemented SBF (SSBF) and immobilized fermentation (IF) using non-detoxified RHSAH, with productivities of 0.16 and 0.37 U mL −1 h −1 , respectively. In addition, our findings revealed that despite having a high RHSAH tolerance, the mutant strain was unable to degrade phenolic compounds. Furthermore, we discovered that inhibitory compounds, including furfural (Fur) and 5 0 -hydroxymethyl furfural (5 0 -HMF), not only inhibit MP biosynthesis, but also regulate the conversion of pigment components.CONCLUSION: The low-cost agricultural by-product, rice husk, can serve as an efficient substitute for MP production with high productivity via IF by Monascus spp.

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The decomposition of lignocellulosic materials by bacteria and their possible application in the environment

Maurya,

Shrivastava,

Avchar

et al. 2025

Sustainable Management of Agro-Food Waste

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Buffalo rumen harbours diverse thermotolerant yeasts capable of producing second-generation bioethanol from lignocellulosic biomass

Cited by 11 publications

References 49 publications

Bioprospecting thermotolerant yeasts from distillery effluent and molasses for high‐temperature ethanol production

Bioprospecting thermotolerant yeasts from distillery effluent and molasses for high‐temperature ethanol production

Utilization of low‐cost agricultural by‐product rice husk for Monascus pigments production via submerged batch‐fermentation

The decomposition of lignocellulosic materials by bacteria and their possible application in the environment

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