Buffer may be the critical factor in measurement of anti-prothrombin antibody on a γ-ray-irradiated plate by enzyme-linked immunosorbent assay

Matsuda, Juzo; Saitoh, Noriko; Tsukamoto, Mitsuo; Gotoh, Moritaka; Gohchi, Kengo; Kawasugi, Kazuo

doi:10.1002/(sici)1096-8652(199612)53:4<242::aid-ajh6>3.0.co;2-y

Cited by 1 publication

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“…Furthermore, the buffer used after fixation of the target antigen to the plate can influence the result [68] and a better standardisation of the tests is mandatory [15,69]. Given a good standardisation it could be debated if these tests have to be repeated for confirmation of the diagnosis APLS because they are (theoretically) not influenced by non-pathogenic antibodies, cancer or drugs.…”

Section: Solid Phase Assays -The Big Hope For More Specific Diagnosticsmentioning

confidence: 99%

The antiphospholipid syndrome and heart valve surgery

Gorki¹,

Malinovski²,

Stanbridge³

2008

European Journal of Cardio-Thoracic Surgery

125

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The antiphospholipid syndrome (APLS) is a complex autoimmune disease often connected to systemic lupus erythematodes. Main features are thromboses, fetal loss and specific antibodies. The involved autoantibodies are directed against plasma proteins such as beta2glycoprotein1 (beta2GPI) or prothrombin which depend on negatively charged phospholipids. Direct antibodies against phospholipids are of no importance for APLS. Clotting tests such as activated partial thromboplastin time or diluted Russell's viper venom test (dRVVT) can show a prolonged time for coagulation despite a prothrombotic state in vivo but the investigator needs awareness about disturbing phospholipid sources and other influential factors. Enzyme linked immuno sorbent assay tests for antibodies against cardiolipin, beta2GPI and prothrombin are valuable solid phase tests with different specificity. Antiphospholipid, anticardiolipin or lupus anticoagulant are misnomers in connection with APLS. They are preserved as a reminiscence of the pioneering work on the way to the still not exactly revealed basics of APLS. Valve operations in APLS patients seem to be rare; a meta-analysis of 57 cases proves that the perioperative management is, at the moment, an empirical approach with high morbidity and mortality in these young patients.

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Section: Solid Phase Assays -The Big Hope For More Specific Diagnosticsmentioning

confidence: 99%