Buffering capacity affects phosphorous solubilization assays in rhizobacteria

Nobahar, Amir; Sarikhani, Mohammad Reza; Chalabianlou, Nastaran

doi:10.1016/j.rhisph.2017.10.002

Cited by 15 publications

(7 citation statements)

References 35 publications

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“…To date, phosphate solubilizing microorganisms are distributed in different bacteria, fungi and actinomycete, P solubilization mechanisms are also different. Many PSMs are mainly through secreting various low-molecular-weight organic acids, which can chelate P via hydroxyl or carboxyl groups and convert insoluble P into soluble forms (HPO4 2and H2PO4 -) (Ahmed & Shahab 2011;Lavania & Nautiyal 2013;Li et al 2015;Nobahar et al 2017). The gluconic and 2-ketogluconic acid were most common organic acids.…”

Section: Discussionmentioning

confidence: 99%

Identification of a Phosphorus-Solubilizing Tsukamurella Tyrosinosolvens Strain and its Effect on the Bacterial Diversity of the Rhizosphere Soil of Peanuts Growth-Promoting

Han

Zhang

2021

Preprint

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Phosphate Solubilizing Microorganisms widely exist in plant rhizosphere soil, but report about the P solubilization and multiple growth-promoting properties of rare actinomycetes are scarce. In this paper, a phosphate solubilizing Tsukamurella tyrosinosolvens P9 strain was isolated from the rhizosphere soil of tea plants. Phosphorus-dissolving abilities of this strain were different under different carbon and nitrogen sources, the soluble phosphorus content was 442.41 mg/L with glucose and potassium nitrate as nutrient sources. The secretion of various organic acids, such as lactic acid, maleic acid, oxalic acid, etc, was the main mechanism for P solubilization and pH value in culture was very significant negative correlation with soluble P content. In addition, this strain had multiple growth-promoting characteristics with 37.26 μg/mL of IAA and 72.01% of siderophore relative content. Under pot experiments, P9 strain improved obviously the growth of peanut seedlings. The bacterial communities of peanut rhizoshpere soil were assessed after inoculated with P9 strain. It showed that there was no significant difference in alpha-diversity indices between the inoculation and control groups, but the P9 treatment group changed the composition of bacterial communities, which increased the relative abundance of beneficial and functional microbes, which relative abundances of Chitinophagaceae and Beijerinckiaceae at the family level, and of Flavihumibacter , Ramlibacter and Microvirga at the genus level, were all siginificant increased. Specially, Tsukamurella tyrosinosolvens were only detected in the rhizosphere of the inoculated group. This study not only founded growth-promoting properties of T . tyrosinosolvens P9 strain and its possible phosphate solublizing mechanism, but also expected to afford an excellent strain resource in biological fertilizers.

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Section: Discussionmentioning

confidence: 99%

Identification of a Phosphorus-Solubilizing Tsukamurella Tyrosinosolvens Strain and its Effect on the Bacterial Diversity of the Rhizosphere Soil of Peanuts Growth-Promoting

Han

Zhang

2021

Preprint

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“…Phytases are widespread in bacteria and have been identified in, for example, Pseudomonas sp. (Irving and Cosgrove, ; Richardson and Hadobas, ; Cho et al ; Nobahar et al ), Klebsiella sp. (Greiner et al ), Enterobacter sp.…”

Section: Introductionmentioning

confidence: 99%

“…The capability of phosphate‐solubilizing bacteria to convert insoluble phosphate to the absorbable form, orthophosphate, is a property of utmost importance for plant growth‐promoting rhizobacteria (Nobahar et al ). Phytate ( myo ‐inositol(1,2,3,4,5,6)hexaphosphate) is considered the most widespread source of phosphate in soils (Rodriguez and Fraga ), and phytases ( myo ‐inositol (1,2,3,4,5,6)hexaphosphate dephosphorylases, E.C.…”

Section: Introductionmentioning

confidence: 99%

Identification of two novel bacterial phosphatase‐encoding genes inPseudomonas putidastrain P13

et al. 2019

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Aims Isolation and identification of genes encoding putative phosphatases from Pseudomonas putida strain P13 DSM 23335. Methods and Results By functional screening of a P. putida P13 genomic library, a number of Pho+ clones were identified. Two genes were identified that encoded proteins exhibiting both phytase and sugar phosphatase activities. The proteins were 249 and 462 amino acids, with molecular masses of 26 and 50 kDa respectively. Sequence alignments revealed no significant similarities to representatives of known phosphatase or phytase gene families. However, the genes were found to have a high similarity to members of the major facilitator superfamily (MFS). Both genes were overexpressed in Escherichia coli and the corresponding partially purified recombinant enzymes were found to have significant phytate‐dephosphorylating activity. The protein designated P. putida phytase 1 (Ppp1) displayed the highest activity among potential substrates studied on Na phytate, whereas Ppp2 more likely represents a sugar phosphatase than a phytase. The optimal conditions for phytate dephosphorylation were determined as 60°C and pH 4·5 (Ppp1) or pH 5·0 (Ppp2). Conclusions Two novel bacterial phosphatase‐encoding genes, named ppp1 and ppp2, were isolated from P. putida P13 DSM 23335 by a functional screening procedure. Significance and Impact of the Study Phosphatase‐encoding genes are of great importance for industrial applications, particularly in agriculture. The identified phosphatase genes represent a new class of acid phosphatases.

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“…Furthermore, fertilization is the most common practice to overcome soil P fixation processes and maintain soil solution P at optimal levels to provide the P demand of agricultural crops and produce them sustainably (Menezes-Blackburn et al 2017;Wei et al 2017). Many complex chemical forms of P differ in mobility and bioavailability, and this affects the fate and transport of soil P (Nobahar et al 2017). Various factors such as the proportional distribution of P in different physicochemical forms, adsorption-desorption reactions, and precipitation-dissolution of P-containing minerals play an important role in converting insoluble P to available P. Knowledge about P fractions and P-containing minerals is necessary to understand the behavior of P in soil and its availability for agricultural crops (Adhami et al 2013;Jalali and Jalali 2016;Shen et al 2019).…”

Section: Introductionmentioning

confidence: 99%

“…Phosphate solubilizing bacteria (PSB) can convert insoluble P to soluble P forms in the rhizosphere. Via its microbial biochemical ability, the P-solubilizing activity has been found to produce and release metabolites such as organic acids (e.g., citrate, malate, oxalate, and gluconate), to acidify the microbial cells and their environment, and to produce phosphatases (Marschner et al 2011;Lagos et al 2016;Nobahar et al 2017;Boroumand et al 2019). The main mechanisms to convert insoluble P to available forms by PSB include (1) the release of complexing or chelating compounds, e.g., organic acids (Rodríguez and Fraga 1999;Barea et al 2005;Hayat et al 2010); (2) the reduction of pH (Gyaneshwar et al 2002;Hayat et al 2010); (3) the release of assimilated P (Sharma et al 2013); (4) the liberation of enzymes (Kaur and Reddy 2015); and (5) the effect on the expression of P transporter genes (Murgese et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Speciation and Fractionation of Phosphorus Affected by Enterobacter cloacae in the Rhizosphere of Sugarcane (Saccharum officinarum L.)

Safirzadeh

Chorom

Enayatizamir

2020

J Soil Sci Plant Nutr

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The purpose of this study is to improve relative phosphorus (P) distribution in various proportions by greater solubility in the rhizosphere of plants influenced by phosphate solubilizing bacteria (PSB), P availability, and fertilizer management. For this purpose, a pot experiment was conducted with three phosphorus concentrations including 0 (P 0), 20.5 (P 50), and 41 (P 100) mg P kg −1 and two PSB strains as well as Enterobacter cloacae R13 (R 13) and E. cloacae R33 (R 33) as factors which were evaluated at three harvesting times: 60, 95, and 140 days after planting (DAP) sowing. In the non-fertilized condition, treatments inoculated by R 13 and R 33 had higher P uptake in comparison with the non-inoculated treatment. Compared with the bulk soil, the chemical composition of the soil solution sample was affected by the dynamic properties of rhizosphere. In the inoculation-treated rhizosphere, the soluble P concentration was higher than that in the non-inoculated treatment. However, P uptake was greater in the inoculated treatments. Also, higher P free ions (H 2 PO 4 − and HPO 4 2−) activity and lower calcium (Ca 2+) and magnesium (Mg 2+) activity were observed in soil solution inoculated rhizosphere as compared with the non-inoculated treatment. At all harvesting times, octacalcium and β-tricalcium phosphate minerals controlled P concentration in the rhizosphere. The pedogenic CaP or low soluble P (Ca 8-P) was higher by up to 72.97% compared with other fractions, which can be ascribed to octacalcium and β-tricalcium phosphate minerals. In the current study, we conclude that the reduction of pH is not a major mechanism in dissolving insoluble P by E. cloacae although phosphate solubility may be attributed to the chelation process. In comparison, rhizosphere characteristics were more effective than PSB in converting the type of P-containing minerals. The results suggest that Ca 8-P fraction is equivalent to octacalcium phosphate and β-tricalcium phosphate minerals in the rhizosphere of sugarcane.

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Buffering capacity affects phosphorous solubilization assays in rhizobacteria

Cited by 15 publications

References 35 publications

Identification of a Phosphorus-Solubilizing Tsukamurella Tyrosinosolvens Strain and its Effect on the Bacterial Diversity of the Rhizosphere Soil of Peanuts Growth-Promoting

Identification of a Phosphorus-Solubilizing Tsukamurella Tyrosinosolvens Strain and its Effect on the Bacterial Diversity of the Rhizosphere Soil of Peanuts Growth-Promoting

Identification of two novel bacterial phosphatase‐encoding genes inPseudomonas putidastrain P13

Speciation and Fractionation of Phosphorus Affected by Enterobacter cloacae in the Rhizosphere of Sugarcane (Saccharum officinarum L.)

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Buffering capacity affects phosphorous solubilization assays in rhizobacteria

Cited by 15 publications

References 35 publications

Identification&nbsp;of a Phosphorus-Solubilizing Tsukamurella Tyrosinosolvens&nbsp;Strain and its Effect on the Bacterial Diversity of the Rhizosphere Soil of Peanuts Growth-Promoting

Identification&nbsp;of a Phosphorus-Solubilizing Tsukamurella Tyrosinosolvens&nbsp;Strain and its Effect on the Bacterial Diversity of the Rhizosphere Soil of Peanuts Growth-Promoting

Identification of two novel bacterial phosphatase‐encoding genes inPseudomonas putidastrain P13

Speciation and Fractionation of Phosphorus Affected by Enterobacter cloacae in the Rhizosphere of Sugarcane (Saccharum officinarum L.)

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Identification of a Phosphorus-Solubilizing Tsukamurella Tyrosinosolvens Strain and its Effect on the Bacterial Diversity of the Rhizosphere Soil of Peanuts Growth-Promoting

Identification of a Phosphorus-Solubilizing Tsukamurella Tyrosinosolvens Strain and its Effect on the Bacterial Diversity of the Rhizosphere Soil of Peanuts Growth-Promoting