Buglossoides spp. seeds, a land source of health-promoting n-3 PUFA and phenolic compounds

Chileh-Chelh, Tarik; Lyashenko, Svetlana; Lahlou, Abdallah; Belarbi, El Hassan; Rincón‐Cervera, Miguel Ángel; Rodrı́guez-Garcı́a, Ignacio; Urrestarazu-Gavilán, Miguel; Ruiz, Rosalía López; Guil‐Guerrero, José Luis

doi:10.1016/j.foodres.2022.111421

Cited by 5 publications

(2 citation statements)

References 36 publications

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“…Rutin and quercetin showed GI 50 values of 680 and 76 µg/mL. Considering that rutin was a major phenolic present in the ethanol extract and its low activity, the recorded antitumor activity was likely due to a synergy between polysaccharides, saponins, and some phenolic compounds, i.e., quercetin, as previously described in Buglossoides seed extracts when tested against HT-29 cancer cells, since these cells are not very sensitive to phenolics [46]. In contrast to rutin, quercetin has been previously demonstrated to inhibit colon cancer cell growth through the induction of apoptosis.…”

Section: Antiproliferative Activity Of the Ethanol Extracts Of Aspara...mentioning

confidence: 60%

Wild Asparagus Shoots Constitute a Healthy Source of Bioactive Compounds

Chileh Chelh,

Rincon-Cervera,

Gomez-Mercado

et al. 2023

Molecules

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Wild Asparagus shoots are consumed worldwide, although most species remain understudied. In this work, a total of four wild Asparagus species were collected from different locations and analyzed compared with farmed A. officinalis. Shoots were screened for (i) phenolic compounds by HPLC-DAD and LC-MS; (ii) total phenolic acids and total flavonoid content by the Folin–Ciocalteu and aluminum chloride methods; (iii) vitamin C by HPLC-DAD; (iv) antioxidant activity by the DPPH and ABTS•+ methods; and (v) the in vitro antiproliferative activities against HT-29 colorectal cancer cells by the MTT assay. Phenolics ranged from 107.5 (A. aphyllus) to 605.4 mg/100 g dry weight (dw) (A. horridus). Vitamin C ranged from 15.8 (A. acutifolius) to 22.7 mg/100 g fresh weight (fw) (A. officinalis). The antioxidant activity was similar in all species, standing out in A. officinalis with 5.94 (DPPH) and 4.64 (ABTS) mmol TE/100 g dw. Among phenolics, rutin reached the highest values (574 mg/100 g dw in A. officinalis), followed by quercetin, nicotiflorin, asterin, and narcissin. The MTT assay revealed the inhibitory effects of ethanol extracts against HT-29 cancer cells, highlighting the cell growth inhibition exercised by A. albus (300 µg/mL after 72 h exposure to cells). This work improves knowledge on the phytochemicals and bioactivities of the shoots of wild Asparagus species and confirms their suitability for use as functional foods.

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Section: Antiproliferative Activity Of the Ethanol Extracts Of Aspara...mentioning

confidence: 60%

Wild Asparagus Shoots Constitute a Healthy Source of Bioactive Compounds

Chileh Chelh,

Rincon-Cervera,

Gomez-Mercado

et al. 2023

Molecules

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“…Therefore, the high bioactivity found for such extracts could be due to a synergy between all the compounds detected. However, it needs to be considered that the polysaccharide-rich fraction in R. nigrum has been characterized as highly bioactive [33], and that the water/methanol extracts obtained from the seeds assayed in this work can include some amounts of polysaccharides; thus, a synergy between phenolic compounds and seed polysaccharides could be also responsible for the noted antitumor effects against HT-29 cells, especially considering that these cells are not too sensitive to phenolic compounds [11,34]. Finally, the selectivity index (SI) of HT-29 versus normal cells CCD-18 was calculated (see Material and Methods, Section 2).…”

Section: Antiproliferative Activity Of the Water/methanol Seed Extrac...mentioning

confidence: 99%

Phenolic Profiles and Antitumor Activity against Colorectal Cancer Cells of Seeds from Selected Ribes Taxa

Lyashenko,

López-Ruiz,

García-Cervantes

et al. 2024

Applied Sciences

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Seeds from several Ribes taxa were surveyed for phenolic compounds and in vitro antiproliferative activity against HT-29 colorectal cancer cells. Total phenolic compounds were analyzed through the Folin–Ciocalteu procedure, while LC coupled to a single mass spectrometer (MS) Orbitrap using an electrospray interface (ESI) was performed to determine the phenolic profiles. Antitumor effects were established using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Total phenolics ranged from 11.4 in R. alpinum to 94.8 mg of caffeic acid equivalents (CAE)/g in R. nigrum ‘Koksa’. Concerning phenolic compounds, four were hydroxylated benzoic acids, four cinnamic acid derivatives, eight flavonoids, and nine flavonoid glycosides. The growth inhibition against HT-29 cancer cells was exercised much better by R. nigrum ‘Koksa’ and Ribes ‘Erkeeni’ (GI50 37 and 42 µg/mL). All Ribes extracts, except for R. nigrum ‘Hara katarlik’, showed higher activity than R. rubrum (GI50 at 72 h: 99 µg/mL). Interestingly, the extract from Ribes ‘Erkeeni’, which exhibited high bioactivity, contains all detected phenolic compounds, unlike R. nigrum ‘Koksa’, which lacks only populnin. Therefore, the high bioactivity found for such extracts could be due to a synergy of all detected compounds. This work constitutes a comprehensive action for expanding knowledge on the phenolic profiles and antitumor activity of GLA-rich Ribes seeds.

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Phenolic profiles and antitumor activity against colorectal cancer cells of seeds from selected Ribes taxa

Lyashenko,

López-Ruiz,

García-Cervantes

et al. 2024

Preprint

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Seeds from several Ribes taxa were surveyed for phenolic compounds and in vitro antiproliferative activity against HT-29 colorectal cancer cells. Total phenolic compounds were analysed through the Folin-Ciocalteu procedure, while phenolic profiles were analysed by LC coupled to a single mass spectrometer Orbitrap using an electrospray interface (ESI). Antitumor effects were established using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Total phenolics ranged from 11.4 in R. alpinum to 94.8 mg of caffeic acid equivalents (CAE)/g in R. nigrum 'Koksa'. Concerning phenolic compounds, four were hydroxylated benzoic acids, four cinnamic acid derivatives, eight flavonoids, and nine flavonoid glycosides. The growth inhibition against HT-29 cancer cells was exercised much better by R. nigrum 'Koksa' and Ribes 'Erkeeni' (GI50 37 and 42 µg/ml). All Ribes extracts, except R. nigrum 'Hara katarlik', showed higher activity than that of R. rubrum (GI50 at 72 h: 99 µg/ml). Interestingly, the highly bioactive extract from Ribes 'Erkeeni' contains all detected phenolics, while R. nigrum 'Koksa' lacks only populnin. Therefore, the high bioactivity found for such extracts could be due to a synergy due to all detected compounds. This work constitutes a comprehensive action for expanding knowledge on the phenolic profiles and antitumor activity of GLA-rich Ribes seeds.

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Buglossoides spp. seeds, a land source of health-promoting n-3 PUFA and phenolic compounds

Cited by 5 publications

References 36 publications

Wild Asparagus Shoots Constitute a Healthy Source of Bioactive Compounds

Wild Asparagus Shoots Constitute a Healthy Source of Bioactive Compounds

Phenolic Profiles and Antitumor Activity against Colorectal Cancer Cells of Seeds from Selected Ribes Taxa

Phenolic profiles and antitumor activity against colorectal cancer cells of seeds from selected Ribes taxa

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