Building a Barrier: The Influence of Different Wax Fractions on the Water Transpiration Barrier of Leaf Cuticles

Seufert, Pascal; Staiger, Simona; Arand, Katja; Bueno, Amauri; Burghardt, Markus; Riederer, Markus

doi:10.3389/fpls.2021.766602

Cited by 20 publications

(12 citation statements)

References 33 publications

(45 reference statements)

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“…However, while total wax was reduced in BW407, changes in composition rather than total wax load may play more important roles in explaining increased cuticular permeability in glossy BW407 tissues. Firstly, across multiple species and tissues, total wax content is not correlated to cuticle permeability [84,85]. Secondly, β-diketone-deficient glossy iw1Iw2 wheat lines also show increased chlorophyll leaching compared to glaucous β-diketone rich lines, despite equivalent total wax in both groups [14].…”

Section: Discussionmentioning

confidence: 99%

A Gene Encoding a SHINE1/WAX INDUCER1 Transcription Factor Controls Cuticular Wax in Barley

et al. 2022

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All land plants seal their above ground body parts with a lipid-rich hydrophobic barrier called the cuticle to protect themselves from dehydration and other terrestrial threats. Mutational studies in several model species have identified multiple loci regulating cuticular metabolism and development. Of particular importance are the eceriferum (cer) mutants characterized by a loss of cuticular wax. Some barley cer mutants, including cer-x, show defects in the distinctive β-diketone-enriched wax bloom on reproductive stage leaf sheaths, stems, and spikes. We exploited extensive allelic populations, near-isogenic lines, and powerful genotyping platforms to identify variation in the HvWAX INDUCER1 (HvWIN1) gene, encoding a SHINE transcription factor, as underlying cer-x. Comparing the cer-x allelic glossy sheath4.l Bowman Near Isogenic Line BW407 to cv. Bowman revealed an increased cuticular permeability in tissues showing reduced accumulation of β-diketones and altered cuticular metabolic gene expression in BW407. Analyses across the barley pangenome and hundreds of exome-capture datasets revealed high sequence conservation of HvWIN1 and two non-synonymous variants exclusive to the cultivated germplasm. Taken together, we suggest that variation in HvWIN1 controls multiple cuticular features in barley.

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Section: Discussionmentioning

confidence: 99%

A Gene Encoding a SHINE1/WAX INDUCER1 Transcription Factor Controls Cuticular Wax in Barley

et al. 2022

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“…However, while total wax was reduced in BW407, changes in cuticular composition rather than total wax load may play more important roles explaining increased cuticular permeability in glossy BW407 tissues. Firstly, across multiple species and tissues, total wax content is not correlated to cuticle permeability (Jetter and Riederer, 2016; Seufert et al , 2022). Secondly, completely β-diketone-deficient glossy iw1Iw2 wheat lines also show increased chlorophyll leaching compared to glaucous β-diketone rich lines, despite equivalent total wax in both groups (Zhang et al , 2013 b ).…”

Section: Discussionmentioning

confidence: 99%

A WAX INDUCER1/SHINE transcription factor controls cuticular wax in barley

McAllister

Campoli

Eskan

et al. 2022

Preprint

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All land plants seal their above ground body parts with a lipid-rich hydrophobic barrier called the cuticle that protects tissues from dehydration and other terrestrial threats. Mutational studies in several model species, including barley, have resolved multiple loci regulating cuticular metabolism and development. Of particular importance are the eceriferum (cer) mutants characterized by visual alterations in cuticular wax. In barley, some cer mutants, such as cer-x lines, show defects in the distinctive β-diketone-enriched wax bloom on reproductive stage leaf sheaths, stems and spikes. In our study we exploited extensive allelic populations, near-isogenic lines and powerful genotyping platforms to identify variation in the HvWAX INDUCER1 (HvWIN1) gene as causal for cer-x mutants. We show that HvWIN1 function reduces cuticular permeability, promotes the accumulation of β-diketones, and regulates cuticular metabolic gene expression. Analyses across the barley pangenome and hundreds of exome-capture datasets revealed high sequence conservation of HvWIN1 but also two non-synonymous variants exclusive to cultivated germplasm. Taken together, we suggest that variation in HvWIN1 controls multiple cuticular features in barley by controlling the expression of genes involved in cuticle development.

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“…In addition to the DGAT1 role in TAG synthesis [ 10 , 68 ], we speculated that overexpression of DGAT1 in HL Lolium affects content of other different lipid classes, including wax esters, either direct or indirect pathways. Wax triterpenoids have been shown to provide mechanical and thermal stability to the plant cuticle; additionally, some reports suggested triterpenoids play no or only a minor role as transpiration barriers [ 71 ]. Further studies on the influence of wax β-amyrin on HL Lolium transpiration may be interesting.…”

Section: Discussionmentioning

confidence: 99%

Insight into the regulatory networks underlying the high lipid perennial ryegrass growth under different irradiances

et al. 2022

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Under favourable conditions, perennial ryegrass (Lolium perenne) engineered to accumulated high lipid (HL) carbon sink in their leaves was previously shown to also enhance photosynthesis and growth. The greater aboveground biomass was found to be diminished in a dense canopy compared to spaced pots. Besides, the underlying genetic regulatory network linking between leaf lipid sinks and these physiological changes remains unknown. In this study, we demonstrated that the growth advantage was not displayed in HL Lolium grown in spaced pots under low lights. Under standard lights, analysis of differentiating transcripts in HL Lolium reveals that the plants had elevated transcripts involved in lipid metabolism, light capturing, photosynthesis, and sugar signalling while reduced expression of genes participating in sugar biosynthesis and transportation. The plants also had altered several transcripts involved in mitochondrial oxidative respiration and redox potential. Many of the above upregulated or downregulated transcript levels were found to be complemented by growing the plants under low light. Overall, this study emphasizes the importance of carbon and energy homeostatic regulatory mechanisms to overall productivity of the HL Lolium through photosynthesis, most of which are significantly impacted by low irradiances.

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Building a Barrier: The Influence of Different Wax Fractions on the Water Transpiration Barrier of Leaf Cuticles

Cited by 20 publications

References 33 publications

A Gene Encoding a SHINE1/WAX INDUCER1 Transcription Factor Controls Cuticular Wax in Barley

A Gene Encoding a SHINE1/WAX INDUCER1 Transcription Factor Controls Cuticular Wax in Barley

A WAX INDUCER1/SHINE transcription factor controls cuticular wax in barley

Insight into the regulatory networks underlying the high lipid perennial ryegrass growth under different irradiances

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