Building a root: the control of patterning and morphogenesis during root development.

Schiefelbein, John; Masucci, James D.; Wang, Haiyang

doi:10.1105/tpc.9.7.1089

Cited by 120 publications

(80 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…For details on HPTS and dye coupling in the Arabidopsis root, see Oparka et al (1994) and Wright and Oparka (1996). See Laux and Jurgens (1997, in this issue) for a discussion of the anatomy of the Arabidopsis embryo; see Schiefelbein et al (1997, in this issue) for a description of the anatomy of Arabidopsis roots.…”

Section: Characterization Of Symplastic Domainsmentioning

confidence: 99%

Plant intercellular communication via plasmodesmata.

McLean¹,

Hempel²,

Zambryski³

1997

Plant Cell

169

103

View full text Add to dashboard Cite

Section: Characterization Of Symplastic Domainsmentioning

confidence: 99%

Plant intercellular communication via plasmodesmata.

McLean¹,

Hempel²,

Zambryski³

1997

Plant Cell

169

103

View full text Add to dashboard Cite

“…Although the inductive regulation of root cell fate has received some notable attention recently (van den Berg et al, 1995;Schiefelbein et al, 1997, in this issue), the physiological and genetic control of root cell elongation per se has been relatively less studied (Baskin et al, 1995;Wu et al, 1996). At the other end of the plant, the dramatic stimulation of shoot elongation in rosette plants by gibberellin has been investigated for decades (reviewed in Kende and Zeevaart, 1997, in this issue).…”

Section: Expansion Leads Mltosls Followsmentioning

confidence: 99%

Why Do Plant Cells Divide?

Jacobs

1997

Plant Cell

View full text Add to dashboard Cite

“…1 borosilicate coverglass that constitutes the base of the chamber, through which they can be observed. Hairs develop in a position-dependent manner on a subset of the epidermal cells of primary roots, and this represents an established model system for the study of cell differentiation (Figure 1) (Schiefelbein et al, 1997;Schneider et al, 1997Schneider et al, , 1998. Movie 1 consists of images taken from a primary root over the time course of the initial development of an individual root hair.…”

Section: Movie 1/figure 1: Root Hair Development In Arabidopsis Thalimentioning

confidence: 99%

Nuclear Dynamics inArabidopsis thaliana

Chytilová

Macas

Śliwińska

et al. 2000

MBoC

118

108

View full text Add to dashboard Cite

The nucleus is a definitive feature of eukaryotic cells, comprising twin bilamellar membranes, the inner and outer nuclear membranes, which separate the nucleoplasmic and cytoplasmic compartments. Nuclear pores, complex macromolecular assemblies that connect the two membranes, mediate communication between these compartments. To explore the morphology, topology, and dynamics of nuclei within living plant cells, we have developed a novel method of confocal laser scanning fluorescence microscopy under time-lapse conditions. This is used for the examination of the transgenic expression in Arabidopsis thaliana of a chimeric protein, comprising the GFP (Green-Fluorescent Protein of Aequorea victoria) translationally fused to an effective nuclear localization signal (NLS) and to ␤-glucuronidase (GUS) from E. coli. This large protein is targeted to the nucleus and accumulates exclusively within the nucleoplasm.This article provides online access to movies that illustrate the remarkable and unusual properties displayed by the nuclei, including polymorphic shape changes and rapid, longdistance, intracellular movement. Movement is mediated by actin but not by tubulin; it therefore appears distinct from mechanisms of nuclear positioning and migration that have been reported for eukaryotes. The GFP-based assay is simple and of general applicability. It will be interesting to establish whether the novel type of dynamic behavior reported here, for higher plants, is observed in other eukaryotic organisms. INTRODUCTIONA definitive feature of eukaryotic cells is the nucleus, first described in stamen cells of Tradescantia by Robert Brown in 1831, which is found in all cell types at some stage of their development. The nucleus comprises twin bilamellar membranes, the inner and outer nuclear membranes, which serve to separate the nuclear contents, the nucleoplasm, from the cytoplasm. Communication between these compartments is mediated by nuclear pores, complex macromolecular assemblies that connect the two membranes.All of our information concerning nuclear morphology, topology, and dynamics comes from the use of various forms of microscopy. The major components of the nucleoplasm, including proteins, RNA, and DNA, do not absorb in the visible part of the spectrum. Thus, nuclei are nearly translucent and nonfluorescent. This impedes the observation of nuclei within living cells with standard bright-field or fluorescence microscopy. Although other forms of light microscopy can be used to examine nuclei, particularly phase contrast and differential interference contrast microscopy, which rely on optical properties other than absorbance and fluorescence, this is practical only in nonpigmented cells and in tissues having simple three-dimensional organization.We have developed an alternative method for observation of nuclei in living plant cells that uses confocal laser scanning fluorescence microscopy (Chytilova et al., 1999). Based on the transgenic expression of the Green Fluorescent Protein (GFP) of Aequorea victoria, it involves ...

show abstract

Building a root: the control of patterning and morphogenesis during root development.

Cited by 120 publications

References 39 publications

Plant intercellular communication via plasmodesmata.

Plant intercellular communication via plasmodesmata.

Why Do Plant Cells Divide?

Nuclear Dynamics inArabidopsis thaliana

Contact Info

Product

Resources

About