Ensuring the correct use of cell lines is crucial to obtaining reliable experimental results and avoiding unnecessary waste of resources. Raman spectroscopy has been confirmed to be able to identify cell lines, but the collection time is usually 10‐30s. In this study, we acquired Raman spectra of five cell lines with integration times of 0.1s and 8s, respectively, and the average accuracy of using long‐short memory neural network to identify the spectra of 0.1s was 95%, and the average accuracy of identifying the spectra of 8s was 99.8%. At the same time, we performed data enhancement of 0.1s spectral data by real‐valued non‐volume preserving method, and the recognition average accuracy of long‐short memory neural networks recognition of the enhanced spectral data was improved to 96.2%. With this method, we shorten the acquisition time of Raman spectra to 1/80 of the original one, which greatly improves the efficiency of cell identification.This article is protected by copyright. All rights reserved.