Building PulseNet International: An Interconnected System of Laboratory Networks to Facilitate Timely Public Health Recognition and Response to Foodborne Disease Outbreaks and Emerging Foodborne Diseases

Swaminathan, Bala; Gerner‐Smidt, Peter; Ng, Lai-King; Lukinmaa, Susanna; Kam, Kai‐Man; Rolando, Sharon; Gutiérrez, Enrique Pérez; Binsztein, Norma

doi:10.1089/fpd.2006.3.36

Cited by 205 publications

(113 citation statements)

References 19 publications

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“…The typing is usually performed in public health laboratories where the endonuclease-cleaved bacterial DNA is separated using pulsed-field gel electrophoresis (PFGE) (2) to produce isolate-specific patterns. The retrospective determination that Escherichia coli O157:H7 isolates from different individuals in a 1993 outbreak had identical PFGE patterns (3) prompted adoption of this technique in North America and elsewhere (2)(3)(4)(5). While PFGE is widely used as a tool in outbreak management, its limitations are emerging.…”

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confidence: 99%

Precise Dissection of an Escherichia coli O157:H7 Outbreak by Single Nucleotide Polymorphism Analysis

et al. 2013

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The current pathogen-typing methods have suboptimal sensitivities and specificities. DNA sequencing offers an opportunity to type pathogens with greater degrees of discrimination using single nucleotide polymorphisms (SNPs) than with pulsed-field gel electrophoresis (PFGE) and other methodologies. In a recent cluster of Escherichia coli O157:H7 infections attributed to salad bar exposures and romaine lettuce, a subset of cases denied exposure to either source, although PFGE and multiple-locus variable-number tandem-repeat analysis (MLVA) suggested that all isolates had the same recent progenitor. Interrogation of a preselected set of 3,442,673 nucleotides in backbone open reading frames (ORFs) identified only 1 or 2 single nucleotide differences in 3 of 12 isolates from the cases who denied exposure. The backbone DNAs of 9 of 9 and 3 of 3 cases who reported or were unsure about exposure, respectively, were isogenic. Backbone ORF SNP set sequencing offers pathogen differentiation capabilities that exceed those of PFGE and MLVA.

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confidence: 99%

Precise Dissection of an Escherichia coli O157:H7 Outbreak by Single Nucleotide Polymorphism Analysis

et al. 2013

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“…Among these genotyping methods, pulsed-field gel electrophoresis (PFGE) has been proven to be a powerful tool for discriminating Shigella strains and has become a standardized method for an international molecular-subtyping network for food-borne-disease surveillance (13). However, PFGE is, at times, too discriminatory for investigating clonal relationships among Shigella strains that have evolved over years or decades.…”

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confidence: 99%

Utility of Multilocus Variable-Number Tandem-Repeat Analysis as a Molecular Tool for Phylogenetic Analysis ofShigella sonnei

Chiou

Watanabe

Wang³

et al. 2009

J Clin Microbiol

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A panel of 916 isolates, including 703 closely related IST1 isolates, were characterized by inter-IS1 spacer typing (IST), pulsed-field gel electrophoresis (PFGE), and multilocus variable-number tandem-repeat (VNTR) analysis (MLVA) to evaluate the utility of MLVA as a molecular tool for the phylogenetic analysis of Shigella sonnei. The global phylogenetic patterns determined by IST, PFGE, and MLVA were concordant. MLVA was carried out using 26 VNTR loci with a range of degrees of variability. MLVA data for the 703 IST1 isolates revealed that diversification among the closely related isolates was attributed mainly to four highly variable loci. The phylogenetic pattern for the closely related isolates determined using MLVA profiles of 8 highly variable loci was in agreement with that determined using the 26-locus profiles. A clustering analysis using the profiles of 18 loci with limited variability established clear phylogenetic relationships among IST clonal groups. Accordingly, MLVA is a useful tool for the phylogenetic analysis of S. sonnei. Combined VNTR loci with higher variability are useful markers for resolving closely related isolates, whereas combined loci with lower variability are suitable for establishing clear phylogenetic relationships between strains or clones that have evolved over a longer timescale.Shigella sonnei is one of the causative agents of shigellosis. Unlike the other three Shigella species, S. dysenteriae, S. flexneri, and S. boydii, which are prevalent in developing countries, S. sonnei is predominant in industrialized countries (6) and is one of the major causes of travel-associated diarrheal disease (3). Transmission of S. sonnei strains between countries occurs frequently via international travel (7,14).The analysis of bacterial isolates by various genotyping methods provides useful information for establishing the genetic relatedness among isolates for the purposes of epidemiological investigation and phylogenetic study. Among these genotyping methods, pulsed-field gel electrophoresis (PFGE) has been proven to be a powerful tool for discriminating Shigella strains and has become a standardized method for an international molecular-subtyping network for food-borne-disease surveillance (13). However, PFGE is, at times, too discriminatory for investigating clonal relationships among Shigella strains that have evolved over years or decades. In order to study an S. sonnei epidemic, we previously developed an inter-IS1 spacer typing (IST) method for subtyping of S. sonnei strains (1). IST is less discriminative than PFGE for S. sonnei, but it is more suitable than PFGE for investigating the clonal relationships among S. sonnei strains in circulation over a short timescale (15).Multilocus sequence typing (MLST) is a sequence-based typing method widely adopted for the phylogenetic study of a number of bacterial pathogens, as listed on the website http: //www.mlst.net/. The MLST method has been used for phylogenetic analysis of Shigella spp. with the protocol developed for Escherichia coli...

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“…PulseNet Europe was created with the same aim in 2003 [8], but it was cancelled in 2006 due to lack of funding [9]. In 2012 the European Centre for Disease Prevention and Control -ECDC developed a pilot Molecular Surveillance System (MSS) as a component of The European Surveillance System -TESSy.…”

Section: Collection Of Listeria Monocytogenes Isolates From Milk Daimentioning

confidence: 99%

Collection of Listeria monocytogenes Isolates from Milk, Dairy Products and Food Processing Environments in Slovakia for the Purposes of European Molecular Database

et al. 2017

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Building PulseNet International: An Interconnected System of Laboratory Networks to Facilitate Timely Public Health Recognition and Response to Foodborne Disease Outbreaks and Emerging Foodborne Diseases

Cited by 205 publications

References 19 publications

Precise Dissection of an Escherichia coli O157:H7 Outbreak by Single Nucleotide Polymorphism Analysis

Precise Dissection of an Escherichia coli O157:H7 Outbreak by Single Nucleotide Polymorphism Analysis

Utility of Multilocus Variable-Number Tandem-Repeat Analysis as a Molecular Tool for Phylogenetic Analysis ofShigella sonnei

Collection of Listeria monocytogenes Isolates from Milk, Dairy Products and Food Processing Environments in Slovakia for the Purposes of European Molecular Database

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