Building the complex architectures of vascular networks: Where to branch, where to connect and where to remodel?

Yin, Jianguo; Heutschi, Daniel; Belting, Heinz‐Georg; Affolter, Markus

doi:10.1016/bs.ctdb.2021.01.002

Cited by 6 publications

(2 citation statements)

References 59 publications

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“…The formation of blood vessels is orchestrated mainly by two different mechanisms: vasculogenesis and angiogenesis, the first occurs in the early stages of embryonic development, from hemangioblasts that will form the primitive vascular plexus and the heart (Dumitrescu et al, 2021), while angiogenesis is the formation of blood vessels from islets of blood by endothelial progenitor cells with the ability to differentiate into vascular cells (Chambers et al, 2021). However, although angiogenesis is the formation of postnatal blood vessels, it has a primary function of vascular remodeling to achieve vascular fusion, that is, the larger blood vessels are reduced and segmented to form primary and secondary vascular plexuses allowing their interconnection and circulation of the blood and its products (Yin et al, 2021;Nitzsche et al, 2022;Ribatti, 2022), inducing the formation of a hierarchical network known as the "Vascular Tree", which includes capillaries, venules, arterioles, veins and arteries (de Silva et al, 2022;Ghanem, 2022).…”

Section: Importance Of Vascular Network In Tissue Regenerationmentioning

confidence: 99%

Biofunctionalization of hydrogel-based scaffolds for vascular tissue regeneration

López-Gutierrez¹,

Ramos‐Payán²,

Ayala-Ham³

et al. 2023

Front. Mater.

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Congenital and acquired tissular losses due to disease or trauma are a major world health problem. Regenerative therapy aims to fix damaged tissues by directing the natural capacity of a host organism to use biofunctionalized artificial tissue scaffolds. These three-dimensional (3D) scaffolds can be customized with cells and/or bioactive molecules to induce cellular homing and angiogenesis, essential to ensure successful tissue regeneration. Hydrogels (HGs) scaffolds are networks of hydrophilic homopolymers, copolymers, and/or macromers with chemical and biological activities that enhance their cell colonization. The use of HGs in regenerative medicine has shown to be advantageous since HGs can be prepared under clinical-grade conditions and tailored to the specific needs of the replaced tissue. They can be made to emulate native extracellular matrices (ECMs) including physical, mechanical, and chemical cues and resilience properties. These customized HGs can reproduce the natural hygroscopic capacity of the original tissue which improves cellular anchoring, nutrition, and waste disposal. They can enable host molecular and cellular modification conducive to a natural cellular microenvironment, modifying the properties of the scaffold, and improving chemotaxis, cell adhesion, migration, proliferation, differentiation, and angiogenesis; HGs can be created and biofunctionalized with linked growth factors and synthetic peptides tailored to positively influence scaffold colonization and functional biocompatibility. This review aims to collect the most relevant information regarding biofunctionalization of HGs used for vascular tissue regeneration, their biological effects, and their clinical implications. While most biofunctionalized HGs are still under investigation, some of them have been studied in vitro, ex vivo, and in vivo with promising results. In this regard, in vivo studies have shown that biofunctionalized scaffolds with peptides such as chitosan hydrogel with LL-37 promotes angiogenesis and healing of pressure ulcers. Also, the GHK tripeptide is widely used in trials focused on guided tissue remodeling.

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Section: Importance Of Vascular Network In Tissue Regenerationmentioning

confidence: 99%

Biofunctionalization of hydrogel-based scaffolds for vascular tissue regeneration

López-Gutierrez¹,

Ramos‐Payán²,

Ayala-Ham³

et al. 2023

Front. Mater.

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show abstract

“…The formation of properly patterned blood vessels needs to be tightly controlled during embryonic development as they provide growing tissues with signaling molecules and the means of gas and metabolite exchange. Early vascular development is often stereotyped and depends on hard-wired genetic programs, while at later stages blood flow patterns provide additional mechanical cues 1 . These include tangential fluid shear stress and circumferential cyclic strain 2 .…”

Section: Introductionmentioning

confidence: 99%

DNA motif analysis of shear stress responsive endothelial enhancers reveals differential association of KLF and ETV/ETS binding sites with gained and lost enhancers

Siekmann

Tsaryk

Yucel

et al. 2021

Preprint

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Endothelial cells (EC) lining blood vessels are exposed to mechanical forces, such as shear stress exerted by the flowing blood. These forces control many aspects of EC biology, including vascular tone, cell migration and proliferation in addition to cell size and shape. Despite a good understanding of the genes and signaling pathways responding to shear stress, our insights into the transcriptional regulation of these responses is much more limited. In particular, we do not know the different sets of regulatory elements (enhancers) that might control increases or decreases in gene expression. Here, we set out to study changes in the chromatin landscape of human umbilical vein endothelial cells (HUVEC) exposed to laminar shear stress. To do so, we performed ChIP-Seq for H3K27 acetylation, indicative of active enhancer elements and ATAC-Seq to mark regions of open chromatin in addition to RNA-Seq on HUVEC exposed to 6 hours of laminar shear stress. Our results show a correlation of gained and lost enhancers with up- and downregulated genes, respectively. DNA motif analysis revealed an over-representation of KLF transcription factor (TF) binding sites in gained enhancers, while lost enhancers contained more ETV/ETS motifs. We validated a subset of flow responsive enhancers using luciferase-based reporter constructs and CRISPR-Cas9 mediated genome editing. Lastly, we characterized shear stress responsive genes in ECs of zebrafish embryos using RNA-Seq. Together, our results reveal the presence of shear stress responsive DNA regulatory elements and lay the groundwork for the future exploration of these elements and the TFs binding to them in controlling EC biology.

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Analysis of the global trade network of the chip industry chain: Does the U.S.-China tech war matter?

Zhang

Zhu

2023

Heliyon

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Building the complex architectures of vascular networks: Where to branch, where to connect and where to remodel?

Cited by 6 publications

References 59 publications

Biofunctionalization of hydrogel-based scaffolds for vascular tissue regeneration

Biofunctionalization of hydrogel-based scaffolds for vascular tissue regeneration

DNA motif analysis of shear stress responsive endothelial enhancers reveals differential association of KLF and ETV/ETS binding sites with gained and lost enhancers

Analysis of the global trade network of the chip industry chain: Does the U.S.-China tech war matter?

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