Buildup of Multilayers Based on Amphiphilic Polyelectrolytes

Guyomard, Aurélie; Müller, G.; Glinel, Karine

doi:10.1021/ma050867n

Cited by 58 publications

(66 citation statements)

References 29 publications

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“…[12] These include electrostatic as well as non-electrostatic interactions, including short-range interactions such as hydrophobicity, [17] hydrogen bonds, [18] Van der Waals forces, charge transfer halogen interactions, [19] and possibly covalent bonds formed by click chemistry. [20] Thus, both the intrinsic properties of the polyelectrolytes themselves (structure of the polyelectrolyte, charge density, chain stiffness) and the physical and chemical properties of the suspending medium (presence and type of salt, pH) are key parameters.…”

Section: Multilayer Formationmentioning

confidence: 99%

Multiple Functionalities of Polyelectrolyte Multilayer Films: New Biomedical Applications

Boudou¹,

Crouzier²,

Ren³

et al. 2010

Advanced Materials

693

702

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The design of advanced functional materials with nanometer- and micrometer-scale control over their properties is of considerable interest for both fundamental and applied studies because of the many potential applications for these materials in the fields of biomedical materials, tissue engineering, and regenerative medicine. The layer-by-layer deposition technique introduced in the early 1990s by Decher, Moehwald, and Lvov is a versatile technique, which has attracted an increasing number of researchers in recent years due to its wide range of advantages for biomedical applications: ease of preparation under "mild" conditions compatible with physiological media, capability of incorporating bioactive molecules, extra-cellular matrix components and biopolymers in the films, tunable mechanical properties, and spatio-temporal control over film organization. The last few years have seen a significant increase in reports exploring the possibilities offered by diffusing molecules into films to control their internal structures or design "reservoirs," as well as control their mechanical properties. Such properties, associated with the chemical properties of films, are particularly important for designing biomedical devices that contain bioactive molecules. In this review, we highlight recent work on designing and controlling film properties at the nanometer and micrometer scales with a view to developing new biomaterial coatings, tissue engineered constructs that could mimic in vivo cellular microenvironments, and stem cell "niches."

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Section: Multilayer Formationmentioning

confidence: 99%

Multiple Functionalities of Polyelectrolyte Multilayer Films: New Biomedical Applications

Boudou¹,

Crouzier²,

Ren³

et al. 2010

Advanced Materials

693

702

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“…[41,42] Polyelectrolyte multilayers were also successfully built by complexing these amphiphilic polyanions with various polycations. [43] The resulting multilayered films were shown to contain hydrophobic domains in which hydrophobic compounds, such as dyes, could be efficiently trapped. Here, we explore the use of these hydrophobically modified polysaccharides to insert a hydrophobic antibacterial peptide into polyelectrolyte films, with the aim of designing a simplified artificial biocidal mucus.…”

Section: Full Papermentioning

confidence: 99%

“…The ability of CMP-xC 10 derivatives to solubilize hydrophobic compounds is related to their associating behavior in aqueous solution. Indeed, amphiphilic chains interact via intra-and/or intermolecular hydrophobic interactions to form aggregates, [43] in which hydrophobic compounds can be trapped. [41,42,49] As the grafting degree in decyl chains x of CMP-xC 10 derivatives increases, their ability to form hydrophobic aggregates and consequently to trap hydrophobic compounds, is enhanced.…”

Section: Solubilization Of the Hydrophobic Peptide By Non-denaturatinmentioning

confidence: 99%

“…The LbL assemblies of PLL and CMP-xC 10 derivatives were already reported elsewhere and were shown to provide stable films. [43] The efficiency of the strategy (Scheme 2) used to incorporate Gram A peptide into the films was checked by performing UV measurement on a 16-bilayer (PLL/CMP L -18C 10 ÃGram A) sample deposited on a fused silica slide (Fig. 3).…”

Section: Insertion Of the Hydrophobic Peptide Into Polyelectrolyte Mumentioning

confidence: 99%

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Incorporation of a Hydrophobic Antibacterial Peptide into Amphiphilic Polyelectrolyte Multilayers: A Bioinspired Approach to Prepare Biocidal Thin Coatings

Guyomard

Dé

Jouenne

et al. 2008

Adv Funct Materials

Self Cite

118

109

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A non‐water‐soluble natural antibacterial peptide, gramicidin A, has been successfully incorporated into polyelectrolyte assemblies to elaborate biocidal thin films. For this, we used a double strategy, the first step of which consists of complexing the peptide by a non‐denaturing anionic amphiphilic polysaccharide, namely a hydrophobically modified carboxymethylpullulan. We demonstrate that the use of this amphiphilic anionic derivative allows to efficiently solubilize the peptide in aqueous solution, without denaturation. The amount of peptide solubilized by the amphiphilic polysaccharide was optimized by systematically varying the hydrophobicity and the molar mass of the CMP derivative. In a second step, the negatively charged complex was layer‐by‐layer assembled with cationic poly(L‐lysine) to form biofunctionalized thin films. The amount of peptide incorporated in the multilayers was controlled by changing the number of deposited complex layers, and was quantified by UV spectroscopy. The antibacterial activity of the resulting biofunctionalized films was evidenced against a gram‐positive bacterium, E. faecalis. We demonstrated that the biocidal activity resulted from a double mechanism: contact between bacteria and the film surface, and release of the peptide into the solution surrounding the film. We also showed that the peptide was not completely removed from the film after rinsing, which insured preservation of the biocidal activity of the film surface.

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“…15 Polysaccharide derivatives (carboxymethyl cellulose, carboxymethylpullulans) have been assembled with synthetic polyelectrolytes (polyethylenimine, polydiallyldimethylammonium) 16 and proteins. 17 LBL of polypeptides (poly-L-lysine, poly-L-glutamic acid) and polysaccharides (chitosan/dextran sulfate) have been assembled onto soft and porous (N-isopropylacrylamide-co-methacrylic acid) microgels.…”

Section: Introductionmentioning

confidence: 99%

Tubular multi‐bilayer polysaccharide biofilms on ultra‐thin cellulose fibers

Ding

Hsieh

2011

J of Applied Polymer Sci

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Multiple bilayered polysaccharide biofilms have been assembled by electrostatic layer-by-layer (LBL), alternating deposition of cationic chitosan (CS, M v ¼ 405 kDa) and anionic dextran sulfate (DXS, M w ¼ 500 kDa) onto ultra-fine cellulose (CELL) and partially hydrolyzed cellulose acetate fibers with diameters ranging from 350 to 410 nm. While the surfaces of partially hydrolyzed (degrees of substitution of 1.14 or 0.2) and CELL fibers were equally hydrophilic, higher surface charges on the more hydrolyzed fibers afford thicker bilayers. The elestrostatic interactions between CS and DXS were enhanced by the presence of NaCl in the dipping and rinsing solutions to allow uniform deposition of sequential polysaccharide bilayers. At 0.25M NaCl, each CS/DXS bilayer averaged 6.4 to 9.0 nm thick with the total thickness of the five bilayer (CS/DXS) 5 varied from 64 to 77 nm. The CS/DXS bilayers exhibited much reduced BET surface area and pore volume indicating that these polysaccharides were much more densely packed on the fully hydrolyzed CELL fibers. The findings proofed the concept that long chain polysaccharide electrolytes can be self-assembled as nanometer scale tubular bilayers on ultra-fine cellulose fibers to afford wholly polysaccharidic fibrous architecture. The electrolytic nature, chemical reactivity, and structural versatility of these ultra-high specific surface polysaccharides are advantageous and can be further tuned to serve biological functions and for biomedical applications.

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Buildup of Multilayers Based on Amphiphilic Polyelectrolytes

Cited by 58 publications

References 29 publications

Multiple Functionalities of Polyelectrolyte Multilayer Films: New Biomedical Applications

Multiple Functionalities of Polyelectrolyte Multilayer Films: New Biomedical Applications

Incorporation of a Hydrophobic Antibacterial Peptide into Amphiphilic Polyelectrolyte Multilayers: A Bioinspired Approach to Prepare Biocidal Thin Coatings

Tubular multi‐bilayer polysaccharide biofilms on ultra‐thin cellulose fibers

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