Bulk and interface investigations of scaffolds and tissue-engineered bones by X-ray microtomography and X-ray microdiffraction

Cancedda, Ranieri; Cedola, Alessia; Giuliani, Alessandra; Komlev, V. S.; Lagomarsino, S.; Mastrogiacomo, Maddalena; Peyrin, Françoise; Rustichelli, F.

doi:10.1016/j.biomaterials.2007.01.022

Cited by 107 publications

(96 citation statements)

References 103 publications

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“…In particular, Xray microfocus computed tomography (micro-CT) has been frequently applied as a 3D quantitative imaging technique to assess the scaffold structure, [6][7][8] as well as bone ingrowth after in vivo implantation. 6,[9][10][11][12][13][14] Furthermore, it has been employed for time-lapsed follow-up of mineralization inside scaffolds during in vitro static [15][16][17] or bioreactor cultures. 13,18,19 In most of these studies, polymeric, ceramic, collagen scaffolds, or composites were used, in which, the mineralized extracellular matrix (ECM) could be separated from the scaffold for the purpose of volume calculations and no significant material-dependent artifacts were present.…”

Section: Introductionmentioning

confidence: 99%

Three-Dimensional Characterization of Tissue-Engineered Constructs by Contrast-Enhanced Nanofocus Computed Tomography

Papantoniou

Sonnaert

Geris

et al. 2014

Tissue Engineering Part C: Methods

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To successfully implement tissue-engineered (TE) constructs as part of a clinical therapy, it is necessary to develop quality control tools that will ensure accurate and consistent TE construct release specifications. Hence, advanced methods to monitor TE construct properties need to be further developed. In this study, we showed proof of concept for contrast-enhanced nanofocus computed tomography (CE-nano-CT) as a whole-construct imaging technique with a noninvasive potential that enables three-dimensional (3D) visualization and quantification of in vitro engineered extracellular matrix (ECM) in TE constructs. In particular, we performed a 3D qualitative and quantitative structural and spatial assessment of the in vitro engineered ECM, formed during static and perfusion bioreactor cell culture in 3D TE scaffolds, using two contrast agents, namely, Hexabrix Ò and phosphotungstic acid (PTA). To evaluate the potential of CE-nano-CT, a comparison was made to standardly used techniques such as Live/Dead viability/cytotoxicity, Picrosirius Red staining, and to net dry weight measurements of the TE constructs. When using Hexabrix as the contrast agent, the ECM volume fitted linearly with the net dry ECM weight independent from the flow rate used, thus suggesting that it stains most of the ECM. When using PTA as the contrast agent, comparing to net weight measurements showed that PTA only stains a part of the ECM. This was attributed to the binding specificity of this contrast agent. In addition, the PTA-stained CE-nano-CT data showed pronounced distinction between flow conditions when compared to Hexabrix, indicating culture-specific structural ECM differences. This novel type of information can contribute to optimize bioreactor culture conditions and potentially critical quality characteristics of TE constructs such as ECM quantity and homogeneity, facilitating the gradual transformation of TE constructs in well-characterized TE products.

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Section: Introductionmentioning

confidence: 99%

Three-Dimensional Characterization of Tissue-Engineered Constructs by Contrast-Enhanced Nanofocus Computed Tomography

Papantoniou

Sonnaert

Geris

et al. 2014

Tissue Engineering Part C: Methods

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“…In contrast to these methods, by micro-CT we can non-destructively obtain 3-D images that penetrate deep into the scaffold interior and produce inherently quantitative results (Tjia & Moghe, 1998). Micro-CT currently appears to be the most suitable approach for this task (Ho & Hutmacher, 2006;Mather et al, 2007;Mather et al, 2008;Cancedda et al, 2007). Clearly, in a structure as complex as bone, micro-CT provides a distinct advantage over conventional microscopy.…”

Section: Imaging and Biological Applications Of Bone Scaffoldsmentioning

confidence: 99%

“…They showed that the quality of the images (and hence the accuracy of any parameters derived from them) may be improved by a combination of pixel binning and by taking multiple images at each angle of rotation. However, micro-CT is considered to be a standard technique in tissue-engineered bones (Cancedda et al, 2007).…”

Section: Imaging and Biological Applications Of Bone Scaffoldsmentioning

confidence: 99%

Correlating Micro-CT Imaging with Quantitative Histology

Gregor¹,

Kochová²,

Eberlová³

et al. 2012

Injury and Skeletal Biomechanics

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“…ceramic or polymeric bone substitution materials. 25,27,31,[57][58][59][60][61][62][63] This field has become very extensive in the last few years, and we want to present just one striking example from developmental biology.…”

Section: Bonementioning

confidence: 99%

X-ray Microcomputer Tomography for the Study of Biomineralized Endo- and Exoskeletons of Animals

Neues

Epple

2008

Chem. Rev.

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Bulk and interface investigations of scaffolds and tissue-engineered bones by X-ray microtomography and X-ray microdiffraction

Cited by 107 publications

References 103 publications

Three-Dimensional Characterization of Tissue-Engineered Constructs by Contrast-Enhanced Nanofocus Computed Tomography

Three-Dimensional Characterization of Tissue-Engineered Constructs by Contrast-Enhanced Nanofocus Computed Tomography

Correlating Micro-CT Imaging with Quantitative Histology

X-ray Microcomputer Tomography for the Study of Biomineralized Endo- and Exoskeletons of Animals

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