2015
DOI: 10.1016/j.aca.2014.11.032
|View full text |Cite
|
Sign up to set email alerts
|

Bulk derivatization and cation exchange restricted access media-based trap-and-elute liquid chromatography–mass spectrometry method for determination of trace estrogens in serum

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

2
30
0

Year Published

2015
2015
2020
2020

Publication Types

Select...
5
2

Relationship

3
4

Authors

Journals

citations
Cited by 27 publications
(32 citation statements)
references
References 44 publications
2
30
0
Order By: Relevance
“…After loading samples on an Oasis HLB (hydrophilic-lipophilic balance) cartridge (Waters, Milford, MA), unconjugated estrogens were first eluted with ethyl acetate, and conjugates were then eluted with methanol containing 0.1% ammonium hydroxide. A more recent study reported an automated on-line trap-and-elute sample treatment process by using a weak cation exchange (WCX) restricted access material (RAM) trap column (Waters) and on-line dilution [52]. This approach has potential application to older men, children, and postmenopausal women samples since the streamlined procedure requires only 100 μL of serum and an LLOQ of 3 pg/mL with excellent accuracy and precision.…”
Section: Lc-tandem Ms (Ms/ms)-based Assays For Estrogen Analysismentioning
confidence: 99%
“…After loading samples on an Oasis HLB (hydrophilic-lipophilic balance) cartridge (Waters, Milford, MA), unconjugated estrogens were first eluted with ethyl acetate, and conjugates were then eluted with methanol containing 0.1% ammonium hydroxide. A more recent study reported an automated on-line trap-and-elute sample treatment process by using a weak cation exchange (WCX) restricted access material (RAM) trap column (Waters) and on-line dilution [52]. This approach has potential application to older men, children, and postmenopausal women samples since the streamlined procedure requires only 100 μL of serum and an LLOQ of 3 pg/mL with excellent accuracy and precision.…”
Section: Lc-tandem Ms (Ms/ms)-based Assays For Estrogen Analysismentioning
confidence: 99%
“…2‐Fluoro‐1‐methylpyridium p ‐toluenesulfonate (2‐FMP) is another reagent that has been used for ISD of estrogens in human and bovine serum . Dns‐Cl was excluded for consideration in this application due to its pH‐dependent charging; 2‐FMP is another reagent that will impart a permanent positive charge onto the derivatized analyte.…”
Section: Alcohols Phenols and Other Hydroxyl‐bearing Compoundsmentioning
confidence: 99%
“…It is therefore crucial to clean the samples prior to LC-MS. This is often accomplished with relatively laborious pretreatments, such as liquid-liquid extraction (LLE) and solid phase extraction (SPE) [41, 42]. These off-line techniques can also suffer from poor recovery and accurancy.…”
Section: Introductionmentioning
confidence: 99%
“…As an example, a reversed phase-based RAM LC-MS/MS system has been used for quantitative determination of dansylated bisphenol in human saliva [65]. A similar approach with RAM trap-and-elute system has been used for trace quantification of estrogens in cerebrospinal fluid and human serum samples [41, 46, 47]. Rao et al .…”
Section: Introductionmentioning
confidence: 99%