Bulk RNA-seq and scRNA-seq analysis reveal an activation of immune response and compromise of secretory function in major salivary glands of obese mice

Huang, Heping; Gao, Ce; Wang, Shuai; Wu, Fen; Wei, Jinsong; Peng, Jinrong

doi:10.1016/j.csbj.2022.11.054

Cited by 6 publications

(3 citation statements)

References 53 publications

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“…Over the past decade, there has been a remarkable rise in various scRNA-seq technologies [ 6 , 7 , 8 , 9 , 10 , 11 , 12 ]. This technology has been widely used in medical science to identify rare cell types or intermediate cells at the single-cell level, to analyze the biological development process of complex cell lineages or embryos, and to explore the pathogenesis of cancer (Reviewed by [ 13 ]).…”

Section: Introductionmentioning

confidence: 99%

Insect Insights at the Single-Cell Level: Technologies and Applications

Sun,

Shao,

Iqbal

2023

Cells

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Single-cell techniques are a promising way to unravel the complexity and heterogeneity of transcripts at the cellular level and to reveal the composition of different cell types and functions in a tissue or organ. In recent years, advances in single-cell RNA sequencing (scRNA-seq) have further changed our view of biological systems. The application of scRNA-seq in insects enables the comprehensive characterization of both common and rare cell types and cell states, the discovery of new cell types, and revealing how cell types relate to each other. The recent application of scRNA-seq techniques to insect tissues has led to a number of exciting discoveries. Here we provide an overview of scRNA-seq and its application in insect research, focusing on biological applications, current challenges, and future opportunities to make new discoveries with scRNA-seq in insects.

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Section: Introductionmentioning

confidence: 99%

Insect Insights at the Single-Cell Level: Technologies and Applications

Sun,

Shao,

Iqbal

2023

Cells

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“…Oligonucleotide microarrays are an established method of obtaining transcriptome data and still complement RNA sequencing 16,17 . Both methods are commonly used to investigate obesity in humans 13,18 and mice [19][20][21][22] . Sex-speci c microarray analysis of rodents' adipose tissues highlighted distinctly different gene expression and male propensity to increased in ammatory responses related to high fat diets 19 .…”

Section: Introductionmentioning

confidence: 99%

“…Considering that other sex-speci c expression differences are also in uenced by obesityrelated diseases 23 it seems standing to reason to investigate male-speci c expression differences across several adipose tissues. As ample transcriptomic comparison data is now available to meaningfully interpret results [19][20][21][22] we aim to do so here.…”

Section: Introductionmentioning

confidence: 99%

On obesogenic diet exposure of parents and sex-specific programming of overweight in mouse offspring

Czechowski,

Hoffmann,

Dommel

et al. 2023

Preprint

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Background/Objectives: Whether parental obesity alters the probability that a child will become an obese adult is not fully understood. Thus, we were interested in exploring how parents’ obesity status influence their offspring’s weight. We further asked whether exposure to milder forms of maternal or paternal obesity during early developmental stages induces programming of long-term overweight in offspring, and if this effect may be sex specific. Subjects/Methods: We randomly assigned three-week-old mice to two groups, one group receiving a high-fat diet (HFD), the other group receiving a control diet (CD). Adult females of both groups were mated to males fed with HFD or CD. Each group consisted of four females and two males (one female deceased). Interventions/methods: After analysing weight gain trajectories of parental (P) and filial (F1) individuals, we investigated F1 transcriptome assay data from four tissues (liver, epigonal visceral, inguinal subcutaneous, and interscapular brown adipose tissue) of 15 male offspring in relation to their parents’ obesity status. Results DEGs were only considered significant when false discovery rate adjusted p values were p < 0.05, and the log2-fold change was at least 1. In brown adipose tissue of male offspring with both parents being obese, when compared to males with only a father obese, expression patterns suggested a higher degree of insulin resistance, impaired muscle growth and regeneration, altered impulse control, and possibly, protection against diet–induced obesity. In liver, the contrast between offspring to obese parents and those to not-obese parents was characterized by expression differences related to fatty acid metabolism, water homeostasis, inflammation and others. Conclusions We provide transcriptomic support for the hypothesis that having two obese parents, rather than having only one obese parent, further amplifies risks for obesity and metabolic disease among males.

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A UTP3-dependent nucleolar translocation pathway facilitates pre-rRNA 5′ETS processing

Bao,

Su,

Chen

et al. 2024

Nucleic Acids Research

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The ribosome small subunit (SSU) is assembled by the SSU processome which contains approximately 70 non-ribosomal protein factors. Whilst the biochemical mechanisms of the SSU processome in 18S rRNA processing and maturation have been extensively studied, how SSU processome components enter the nucleolus has yet to be systematically investigated. Here, in examining the nucleolar localization of 50 human SSU processome components, we found that UTP3, together with another 24 proteins, enter the nucleolus autonomously. For the remaining 25 proteins we found that UTP3/SAS10 assists the nucleolar localization of five proteins (MPP10, UTP25, EMG1 and the two UTP-B components UTP12 and UTP13), likely through its interaction with nuclear importin α. This ‘ferrying’ function of UTP3 was then confirmed as conserved in the zebrafish. We also found that knockdown of human UTP3 impairs cleavage at the A0-site while loss-of-function of either utp3/sas10 or utp13/tbl3 in zebrafish causes the accumulation of aberrantly processed 5′ETS products, which highlights the crucial role of UTP3 in mediating 5′ETS processing. Mechanistically, we found that UTP3 facilitates the degradation of processed 5′ETS by recruiting the RNA exosome component EXOSC10 to the nucleolus. These findings lay the groundwork for studying the mechanism of cytoplasm-to-nucleolus trafficking of SSU processome components.

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Bulk RNA-seq and scRNA-seq analysis reveal an activation of immune response and compromise of secretory function in major salivary glands of obese mice

Cited by 6 publications

References 53 publications

Insect Insights at the Single-Cell Level: Technologies and Applications

Insect Insights at the Single-Cell Level: Technologies and Applications

On obesogenic diet exposure of parents and sex-specific programming of overweight in mouse offspring

A UTP3-dependent nucleolar translocation pathway facilitates pre-rRNA 5′ETS processing

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