Bull subfertility is associated with low levels of a sperm membrane antigen

Parent, Sophie; Lefièvre, Linda; Brindle, Yves; Sullivan, Robert

doi:10.1002/(sici)1098-2795(199901)52:1<57::aid-mrd8>3.3.co;2-l

Cited by 14 publications

(18 citation statements)

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“…Many studies have correlated sperm parameters and bull fertility with some sperm proteins. For example, osteopontin (OPN), BSP 30 kDa, phospholipase A2, heparin-binding proteins (HBP), and P25b are positively correlated with fertility [32] [33]. On the other hand, spermadhesin z13 and clusterin showed a negative correlation [33] [34].…”

Section: Discussionmentioning

confidence: 99%

Characterization of Angiotensin-Converting Enzyme before and after Cryopreservation of Gir Semen

Furtado¹,

Faria²,

Santos³

et al. 2019

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The aim of this study was to characterize the angiotensin-converting enzyme (ACE) in Gir semen before and after cryopreservation. The ejaculate of five sexually mature bulls was used. After collection, one 1-mL aliquot of fresh semen was analyzed immediately, and the rest of the semen was cryopreserved in liquid nitrogen for subsequent analysis. Freshly collected semen and thawed cryopreserved semen were centrifuged twice with Tyrode's albumin lactate pyruvate medium (TALP) to remove plasma and extender, respectively. Samples were then subjected to western blotting, immunocytochemistry, and enzymatic activity techniques. At least one 100 kDa band was observed in every bull analyzed using western blotting with an anti-ACE monoclonal antibody, and band intensity decreased by 70% (p < 0.05) after cryopreservation. Immunocytochemistry showed periacrosomal ACE localization, and the area stained by the fluorescent antibody significantly decreased (p < 0.05) after cryopreservation. Enzyme activity was evaluated using FAPGG substrate hydrolysis, which was significantly lower (p < 0.05) in cryopreserved semen than in fresh semen. Therefore, the process of cryopreservation decreases ACE band intensity and enzyme activity in Gir bull semen, and reduces the stained area in immunocytochemistry.

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Section: Discussionmentioning

confidence: 99%

Characterization of Angiotensin-Converting Enzyme before and after Cryopreservation of Gir Semen

Furtado¹,

Faria²,

Santos³

et al. 2019

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“…P34H has been shown to be antigenically and functionally similar to the hamster P26h protein (13). When a P26h antiserum was used against bovine sperm, two proteins were identified; P25b and P21b (70). Similar to P34H,…”

Section: Sperm Proteins In Other Speciesmentioning

confidence: 99%

“…P25b is localized to the acrosomal cap (70). P21b, on the other hand, is associated with the flagellum (70).…”

Section: Sperm Proteins In Other Speciesmentioning

confidence: 99%

Effect of season on sperm membrane protein 22 and selected mRNAs in fresh and cryopreserved stallion sperm

Wrench

Pinto

Klinefelter

et al. 2008

Animal Reproduction Science

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“…Seminal fructose and glucose are essential source of energy production and motility of spermatozoa (Jobim et al, 2004;Assumpcao et al, 2005;Garner et al, 2001). The potential influence of seminal proteins on male reproduction came to attention because of the studies showing that their expression is associated with breeding scores of dairy bulls (Killian et al 1993;Cancel et al 1997), beef bulls (Bellin et al, 1994(Bellin et al, , 1996Parent et al, 1999) and horses (Brandon et al, 1999) The protein composition of mammalian seminal plasma varies among species and has important effects on sperm function (Villemure et al, 2003;Mortarino et al, 1998;Cross, 1993;Miller et al, 1990;Strzezek et al, 2002). The bulk of the proteins found in seminal plasma derive from the seminal vesicles, although albumin is mainly of prostatic origin (Hirsch et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

Biochemical analysis of bovine (BosIndicus) seminal plasma

Khan

Yasinzai

Kakar

2015

Int. J. Adv. Biol. Biomed. Res.

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This article was published in an CASRP journal. The attached copy is furnished to the author for non-commercial research and education use, including for instruction at the authors institution, sharing with colleagues and providing to institution administration. Other uses, including reproduction and distribution, or selling or licensing copied, or posting to personal, institutional or third party websites are prohibited. In most cases authors are permitted to post their version of the article (e.g. in Word or Tex form) to their personal website or institutional repository. Authors requiring further information regarding CASRP΄s archiving and manuscript policies encouraged to visit: http://www.casrp.co.uk/journals

show abstract

Bull subfertility is associated with low levels of a sperm membrane antigen

Cited by 14 publications

References 0 publications

Characterization of Angiotensin-Converting Enzyme before and after Cryopreservation of Gir Semen

Characterization of Angiotensin-Converting Enzyme before and after Cryopreservation of Gir Semen

Effect of season on sperm membrane protein 22 and selected mRNAs in fresh and cryopreserved stallion sperm

Biochemical analysis of bovine (BosIndicus) seminal plasma

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