Bullera megalospora, a new species of yeast forming large ballistospores isolated from dead leaves of Oryza sativa, Miscanthus sinensis and Sasa sp. in Japan.

Nakase, Takashi; Suzuki, Motofumi

doi:10.2323/jgam.32.225

Cited by 135 publications

(110 citation statements)

References 8 publications

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“…nitrogen compounds was examined on solid media using a starved inoculum (Nakase & Suzuki, 1986). Vitamin requirements were investigated according to the method of Komagata & Nakase (1967).…”

Section: Methodsmentioning

confidence: 99%

Rhodotorula benthica sp. nov. and Rhodotorula calyptogenae sp. nov., novel yeast species from animals collected from the deep-sea floor, and Rhodotorula lysiniphila sp. nov., which is related phylogenetically

Nagahama

Hamamoto

Nakase

et al. 2003

International Journal of Systematic and Evolutionary Microbiology

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Section: Methodsmentioning

confidence: 99%

Rhodotorula benthica sp. nov. and Rhodotorula calyptogenae sp. nov., novel yeast species from animals collected from the deep-sea floor, and Rhodotorula lysiniphila sp. nov., which is related phylogenetically

Nagahama

Hamamoto

Nakase

et al. 2003

International Journal of Systematic and Evolutionary Microbiology

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“…Morphological, physiological and biochemical characteristics were examined according to standard methods commonly used in yeast taxonomy (Yarrow, 1998). Assimilation of nitrogen compounds was investigated on solid media with starved inocula (Nakase & Suzuki, 1986).Nuclear DNA was extracted by the method of Makimura et al (1994). Sequencing of the internal transcribed spacer (ITS) region (including 5.8S rDNA) and the large-subunit rDNA D1/D2 domain, and molecular phylogenetic analysis were performed as described previously (Wu et al, 2006).…”

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confidence: 99%

Candida cellulosicola sp. nov., a xylose-utilizing anamorphic yeast from rotten wood

Guo

Zhu

Bai

2012

International Journal of Systematic and Evolutionary Microbiology

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Two xylose-utilizing yeast strains isolated from rotten wood collected in the rainforest in different mountains of Hainan province, southern China, were studied. Sequence analysis of the large subunit rDNA D1/D2 domain and internal transcribed spacer region revealed that the strains represent a novel anamorphic yeast species, for which the name Candida cellulosicola sp. nov. is proposed; the type strain is HNX16-2 T (5CGMCC 2.3503 T 5CBS 11952 T ). Phylogenetically, the novel species was closely related to a xylose-utilizing teleomorphic ascomycetous yeast species Spencermartinsiella europaea in the family Trichomonascaceae, but differed from the latter by 3.0 % mismatches in the D1/D2 domain.Forest habitats such as rotten wood and plant litter containing plenty of assimilable carbon compounds are expected to harbour diverse yeasts, including pentoseutilizing or -fermenting species. These yeasts are of potential value in bio-ethanol production from lignocellulose materials. During an ecological survey on microfungi colonizing rotten wood in the rainforest of southern Chile, Dill et al. (1984) and Ramírez & González (1984a, b, c) described a total of 18 novel yeast species (Middelhoven, 2006). Of the six novel methanol-assimilating ascomycetous yeast species from wood material described by Péter et al. (2003), four were isolated from rotten wood. Middelhoven & Kurtzman (2007) identified two novel ascomycetous yeast species from strains isolated from rotten wood. In recent years, new xylose-or cellobiose-fermenting yeast species have been isolated from rotting wood in Brazil and the Atlantic rainforest (Barbosa et al., 2009;Cadete et al., 2009;Santos et al., 2011). In an effort to investigate the biodiversity of yeasts inhabiting rotten wood in the rainforest of Hainan, a tropical island in southern China, a variety of undescribed yeast species were isolated ; two of them have been described previously . A further novel anamorphic ascomycetous yeast species represented by two of the Hainan strains from rotten wood is described in this study.Yeasts were isolated using the enrichment method selective for xylose-utilizing strains. Rotten wood samples were put in enrichment medium containing: 1 % (w/v) yeast nitrogen base (Difco), 2 % (w/v) xylose and 200 mg chloromycetin ml 21 . After incubation at 25 u C for 7-14 days, aliquots (200 ml) of 10 21 to 10 23 diluted enrichment medium were spread on agar plates that were prepared using enrichment medium plus 2 % agar. After incubation of the plates for 2-5 days at 25 u C, yeast colonies with different morphological characters were selected for further study.Strains HNX16-2 T and HNX35-3 were isolated from two different rotten wood samples of broad-leaved trees collected in Wuzhi and Diaoluo Mountains, respectively. The two mountains, which are approximately 30 km apart, are located in Hainan province, southern China. Morphological, physiological and biochemical characteristics were examined according to standard methods commonly used in yeast taxonomy (Yarrow, 1998). ...

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“…Growth at various temperatures was determined by cultivation on YM broth (3 g yeast extract l 21 , 3 g malt extract l 21 , 5 g peptone l 21 , 10 g glucose l 21 ) and YM agar (YM broth containing 20 g agar l 21 ), using a water bath and an incubator, respectively. Ubiquinones were extracted from intact packed cells and purified according to the method described by Nakase & Suzuki (1986). The isoprenologues were identified by an HPLC system (Agilent 1100) using a Cosmosil (Waters 5C18) 4?66250 mm column and methanol/isopropanol (2 : 1) at 1 ml min 21 as the elution system, with spectrophotometric detection (wavelength 275 nm).…”

mentioning

confidence: 99%

“…Assimilation of nitrogen compounds was examined on solid media with starved inocula, according to the method of Nakase & Suzuki (1986). Growth at various temperatures was determined by cultivation on YM broth (3 g yeast extract l 21 , 3 g malt extract l 21 , 5 g peptone l 21 , 10 g glucose l 21 ) and YM agar (YM broth containing 20 g agar l 21 ), using a water bath and an incubator, respectively.…”

mentioning

confidence: 99%

Pichia thermomethanolica sp. nov., a novel thermotolerant, methylotrophic yeast isolated in Thailand

Limtong

Srisuk

Yongmanitchai

et al. 2005

International Journal of Systematic and Evolutionary Microbiology

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Three strains (N002, N069 and PT31 T ) of a novel thermotolerant methylotrophic yeast species belonging to the genus Pichia were isolated from soil collected in Thailand by three consecutive enrichments in methanol broth at room temperature. They were categorized as thermotolerant strains on the basis of their good growth below 20 6C and up to a high temperature (37 6C). The major characteristics of the three strains included the following and placed them in the genus Pichia: the formation of four helmet-/hat-shaped ascospores in a deliquescent ascus that might be unconjugated or produced by conjugation between a cell and its bud or between independent cells; multilateral budding; the presence of ubiquinone Q-7; negative for Diazonium blue B colour and urease reactions; and the absence of arthrospores and ballistospores. The three strains differed by one to three nucleotide substitutions in the sequences of the D1/D2 domain of the large-subunit rDNA sequence. Phylogenetic analysis revealed that their closest species was Pichia dorogensis, but with 11-13 nucleotide substitutions in 554 nt. The phenotypic characteristics of the three strains were the same. The strains could be distinguished from P. dorogensis by a number of phenotypic characteristics. On the basis of the above findings, these three strains were assigned to a single novel species of Pichia, for which the name Pichia thermomethanolica sp. nov. is proposed. The type strain is PT31 T (=BCC 16875 T =JCM 12984 T =CBS 10098 T ).Methanol metabolism is a characteristic shown by relatively few yeast species (the methylotrophic yeasts); most known methylotrophic yeasts belong to the genera Pichia and Candida (Kurtzman & Fell, 1998;Barnett et al., 2000). From the differences in the partial sequences of small-subunit (18S) and large-subunit (26S) rDNA sequence, Yamada et al. (1994) proposed the transfer of hat-shaped ascosporeforming, nitrate-assimilating methylotrophic yeast species formerly classified in the genus Pichia (Pichia angusta, Pichia minuta var. minuta, P. minuta var. nonfermentans, Pichia philodendra, Pichia glucozyma and Pichia henricii) to a newly described genus, Ogataea. Thereafter, Mikata & Yamada (1995) transferred Pichia kodamae to the genus Ogataea.Recently, Morais et al. (2004) described Ogataea falcaomoraisii as a novel sporogenous methylotrophic yeast, on the basis of the sequences of the D1/D2 domain of the 26S rDNA. However, the circumscription of Ogataea is not generally accepted. Kurtzman & Robnett (1998) suggested that a more robust dataset is required in order to substantiate the circumscription.Methylotrophic yeasts have attracted interest since they were first isolated for both physiological study and industrial applications (Levine & Cooney, 1973;Demain et al., 1998). Recently, some of them have become the principal biocatalyst for the production of useful compounds, and are an important host for the expression of genes (Sakai et al., 1996;Gellissen, 2000). Thermotolerant or thermophilic micro-organisms have certain advantage...

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Bullera megalospora, a new species of yeast forming large ballistospores isolated from dead leaves of Oryza sativa, Miscanthus sinensis and Sasa sp. in Japan.

Cited by 135 publications

References 8 publications

Rhodotorula benthica sp. nov. and Rhodotorula calyptogenae sp. nov., novel yeast species from animals collected from the deep-sea floor, and Rhodotorula lysiniphila sp. nov., which is related phylogenetically

Rhodotorula benthica sp. nov. and Rhodotorula calyptogenae sp. nov., novel yeast species from animals collected from the deep-sea floor, and Rhodotorula lysiniphila sp. nov., which is related phylogenetically

Candida cellulosicola sp. nov., a xylose-utilizing anamorphic yeast from rotten wood

Pichia thermomethanolica sp. nov., a novel thermotolerant, methylotrophic yeast isolated in Thailand

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