Bupivacaine Injection Leads to Muscle Force Reduction and Histologic Changes in a Murine Model

Ingham, Sheila Jean McNeill; Pochini, Alberto de Castro; Oliveira, Daniel Antonio de; Lisboa, Bianca Cristina Garcia; Beutel, Abram; Valero-Lapchik, Valderez Bastos; Ferreira, Ana Maria Reis; Abdalla, René Jorge; Cohen, Moisés; Han, Sang Won

doi:10.1016/j.pmrj.2011.05.027

Cited by 7 publications

(5 citation statements)

References 17 publications

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“…6) caused by the surgically-induced injury. Our results corroborate previous data reported in a toxin-induced muscle injury rat model, where a 37 % decrease in Tetanic force was described in rat gastrocnemius muscle 2 weeks after injury induced by intramuscular bupivacaine injection [24]. In summary, this study presents a new model of surgicallyinduced skeletal muscle injury in rats, which mimics the complete process of necrosis and regeneration observed in muscle injuries and reproduces very closely the most frequent skeletal muscle lesions observed in sports human clinics.…”

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confidence: 91%

A New Surgical Model of Skeletal Muscle Injuries in Rats Reproduces Human Sports Lesions

et al. 2015

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Skeletal muscle injuries are the most common sports-related injuries in sports medicine. In this work, we have generated a new surgically-induced skeletal muscle injury in rats, by using a biopsy needle, which could be easily reproduced and highly mimics skeletal muscle lesions detected in human athletes. By means of histology, immunofluorescence and MRI imaging, we corroborated that our model reproduced the necrosis, inflammation and regeneration processes observed in dystrophic mdx-mice, a model of spontaneous muscle injury, and realistically mimicked the muscle lesions observed in professional athletes. Surgically-injured rat skeletal muscles demonstrated the longitudinal process of muscle regeneration and fibrogenesis as stated by Myosin Heavy Chain developmental (MHCd) and collagen-I protein expression. MRI imaging analysis demonstrated that our muscle injury model reproduces the grade I-II type lesions detected in professional soccer players, including edema around the central tendon and the typically high signal feather shape along muscle fibers. A significant reduction of 30% in maximum tetanus force was also registered after 2 weeks of muscle injury. This new model represents an excellent approach to the study of the mechanisms of muscle injury and repair, and could open new avenues for developing innovative therapeutic approaches to skeletal muscle regeneration in sports medicine.

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confidence: 91%

A New Surgical Model of Skeletal Muscle Injuries in Rats Reproduces Human Sports Lesions

et al. 2015

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“…To make an animal model which will explain the correlation between the paraspinal muscle injury and kyphosis, we have reviewed the muscle injury models introduced to date. The bupivacaine is the most widely used material, the injection of bupivacaine weakens the strength of skeletal muscles and this induces the necrosis of muscle fibers in histology 17) . Besides, it was reported that venom phospholipases A2 induces the necrosis of skeletal muscle 9) .…”

Section: Discussionmentioning

confidence: 99%

Chronic Paraspinal Muscle Injury Model in Rat

Cho

Park

Kim

2016

J Korean Neurosurg Soc

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ObjectiveThe objective of this study is to establish an animal model of chronic paraspinal muscle injury in rat.MethodsFifty four Sprague-Dawley male rats were divided into experimental group (n=30), sham (n=15), and normal group (n=9). Incision was done from T7 to L2 and paraspinal muscles were detached from spine and tied at each level. The paraspinal muscles were exposed and untied at 2 weeks after surgery. Sham operation was done by paraspinal muscles dissection at the same levels and wound closure was done without tying. Kyphotic index and thoracolumbar Cobb's angle were measured at preoperative, 2, 4, 8, and 12 weeks after the first surgery for all groups. The rats were sacrificed at 4, 8, and 12 weeks after the first surgery, and performed histological examinations.Results At 4 weeks after surgery, the kyphotic index decreased, but, Cobb's angle increased significantly in the experimental group (p<0.05), and then that were maintained until the end of the experiment. However, there were no significant differences of the kyphotic index and Cobb's angle between sham and normal groups. In histological examinations, necrosis and fibrosis were observed definitely and persisted until 12 weeks after surgery. There were also presences of regenerated muscle cells which nucleus is at the center of cytoplasm, centronucleated myofibers.ConclusionOur chronic injury model of paraspinal muscles in rats shows necrosis and fibrosis in the muscles for 12 weeks after surgery, which might be useful to study the pathophysiology of the degenerative thoracolumbar kyphosis or degeneration of paraspinal muscles.

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“…In rabbit studies, 8 to 10% lidocaine has been demonstrated to be neurotoxic [22, 23]. The other local anesthetic, bupivacaine, has been shown to be degenerative [24] and to decrease muscle function [25] in rats. These studies also showed that, over time, bupivacaine was not completely destructive but had some regenerative and healing effects.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity of local anesthetics and nonionic contrast agents on bovine intervertebral disc cells cultured in a three-dimensional culture system

et al. 2014

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Bupivacaine Injection Leads to Muscle Force Reduction and Histologic Changes in a Murine Model

Abstract: Although our results do not allow a direct clinical application, we believe that caution should be warranted when intramuscular bupivacaine is used.

Cited by 7 publications

References 17 publications

A New Surgical Model of Skeletal Muscle Injuries in Rats Reproduces Human Sports Lesions

A New Surgical Model of Skeletal Muscle Injuries in Rats Reproduces Human Sports Lesions

Chronic Paraspinal Muscle Injury Model in Rat

Cytotoxicity of local anesthetics and nonionic contrast agents on bovine intervertebral disc cells cultured in a three-dimensional culture system

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